Date published: 2025-9-9

021-6093-6350

SCBT Portrait Logo
Seach Input

Pit-1 抗体 (D-7): sc-393943

5.0(6)
寫評論提問

说明书
  • Pit-1抗体(D-7)是小鼠单克隆IgG2a κ, 在17篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Pit-1的N-terminus的氨基酸5-32之间
  • 抗-Pit-1 抗体 (D-7) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Pit-1
  • 抗Pit-1抗体(D-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-393943 X, 200 µg/0.1 ml)
  • 关于如何获取Pit-1 (D-7): sc-393943免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP是Pit-1 Antibody (D-7) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Pit-1 Antibody (D-7) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

Pit-1抗体(D-7)是一种IgG2a κ小鼠单克隆Pit-1抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的Pit-1。Pit-1抗体(D-7)可作为非偶联抗Pit-1抗体形式提供,也可作为多种偶联形式的抗Pit-1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。转录调控因子通过介导组织和细胞特异性转录在发育过程中发挥关键作用。POU结构域因子是转录调控因子,其特征是具有一个由两个高度保守的区域组成的二分DNA结合域,这两个区域由14-26个氨基酸的可变连接体连接。Pit-1,也称为生长激素因子-1(GHF-1),是POU同源结构域家族的一员,对于垂体前叶的正常发育至关重要,它是形成生长素细胞、催乳素细胞和促甲状腺激素细胞所必需的。在生长素细胞和催乳素细胞中,Pit-1分别激活生长激素和催乳素的产生。此外,Pit-1作为基因表达的抑制因子,允许特定细胞类型的分化。Pit-1表达为两种选择性剪接产物,分别命名为Pit-1a和Pit-1b,它们在反式激活能力上有所不同。Pit-1基因的突变被认为会导致生长激素、催乳素和促甲状腺激素的联合垂体激素缺乏症(CPHD)。编码Pit-1的基因定位于人类3号染色体3p11上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Pit-1 抗体 (D-7) 参考文献:

  1. GHRH 对体细胞的增殖作用具有细胞类型特异性,并依赖于 Pit-1/GHF-1 的表达。  |  Solloso, A., et al. 2008. J Cell Physiol. 215: 140-50. PMID: 17941086
  2. PIT-1(POU1F1)在体细胞泌乳细胞增殖和凋亡中的剂量依赖性双重作用  |  Jullien, N., et al. 2015. PLoS One. 10: e0120010. PMID: 25822178
  3. 转录因子 Pit-1 影响双启动子人类催乳素基因的转录时序  |  McNamara, AV., et al. 2021. Endocrinology. 162: PMID: 33388754
  4. PD-L1在PIT-1阳性垂体神经内分泌肿瘤中优先表达  |  Turchini, J., et al. 2021. Endocr Pathol. 32: 408-414. PMID: 33694064
  5. 一种侵袭性分化不良的多激素 Pit-1 阳性腺瘤。  |  Postma, MR., et al. 2021. Endocrinol Diabetes Metab Case Rep. 2021: PMID: 34673544
  6. 膦甲酸通过 Pit-1 减少高磷血症诱发的血管钙化  |  Zang, H., et al. 2024. J Int Med Res. 52: 3000605231222156. PMID: 38180904
  7. 单层培养的大鼠垂体前叶细胞中生长激素分泌诱导剂对垂体特异性转录因子 GHF-1/Pit-1 信使核糖核酸水平的调节。  |  Soto, JL., et al. 1995. Endocrinology. 136: 3863-70. PMID: 7649093
  8. 在 HeLa 非垂体细胞中重建蛋白激酶 A 对大鼠催乳素启动子的调控:识别依赖 GHF-1/Pit-1 和不依赖 GHF-1/Pit-1 的机制。  |  Rajnarayan, S., et al. 1995. Mol Endocrinol. 9: 502-12. PMID: 7659093
  9. 垂体转录因子 GHF-1/PIT-1 在成年猪腺垂体细胞类型中的表达。  |  Malagon, MM., et al. 1996. J Histochem Cytochem. 44: 621-7. PMID: 8666747
  10. 转录因子 GHF-1(而非剪接变体 GHF-2)与甲状腺激素和视黄酸受体合作刺激大鼠生长激素基因的表达。  |  Sánchez-Pacheco, A., et al. 1998. FEBS Lett. 422: 103-7. PMID: 9475179

订购信息

产品名称产品编号规格价格数量收藏夹

Pit-1 抗体 (D-7)

sc-393943
200 µg/ml
RMB2377.00

Pit-1 (D-7): m-IgG2a BP-HRP 套装

sc-545968
200 µg Ab; 10 µg BP
RMB2662.00

Pit-1 抗体 (D-7) X

sc-393943 X
200 µg/0.1 ml
RMB2377.00

Pit-1 抗体 (D-7) AC

sc-393943 AC
500 µg/ml, 25% agarose
RMB3129.00

Pit-1 抗体 (D-7) HRP

sc-393943 HRP
200 µg/ml
RMB2377.00

Pit-1 抗体 (D-7) FITC

sc-393943 FITC
200 µg/ml
RMB2482.00

Pit-1 抗体 (D-7) PE

sc-393943 PE
200 µg/ml
RMB2580.00

Pit-1 抗体 (D-7) Alexa Fluor® 488

sc-393943 AF488
200 µg/ml
RMB2685.00

Pit-1 抗体 (D-7) Alexa Fluor® 546

sc-393943 AF546
200 µg/ml
RMB2685.00

Pit-1 抗体 (D-7) Alexa Fluor® 594

sc-393943 AF594
200 µg/ml
RMB2685.00

Pit-1 抗体 (D-7) Alexa Fluor® 647

sc-393943 AF647
200 µg/ml
RMB2685.00

Pit-1 抗体 (D-7) Alexa Fluor® 680

sc-393943 AF680
200 µg/ml
RMB2685.00

Pit-1 抗体 (D-7) Alexa Fluor® 790

sc-393943 AF790
200 µg/ml
RMB2685.00

Pit-1 (D-7) 中和勝肽

sc-393943 P
100 µg/0.5 ml
RMB511.00

hi, We have purchased D7 sc393943 por formalin fixed paraffin embedded tissues on Ventana benchmark ultra platform. Does it work? At what dilution? pretreatment etc? Thks

Asked by: Dimitris
Thank you for your question. D7 antibody (sc-393943) has not been specifically validated for use on the Ventana Benchmark Ultra platform with formalin-fixed, paraffin-embedded (FFPE) tissues. However, customers have reported successful results with this antibody in immunohistochemistry (IHC) applications on FFPE samples.
Answered by: Tech Support Europe
Date published: 2025-05-21

Hello, Could you please send me the SDS for Anti-Pit-1 Antibody (D-7): sc-393943? Thank you.

Asked by: Aleksandra1234
Thank you for your question. Our antibody products are non-hazardous and do not require SDS documentation. You can find the product datasheet right here on the product site. For further information, please reach out to our technical service team directly.
Answered by: Tech Support Europe
Date published: 2022-06-22

Dear Team Could I please request a quote for the IHC P concentrate?

Asked by: Sandie Iles
Thank you for your question. For accurate availability, shipping and pricing information regarding our products, or to receive a quote, please contact Customer Service or reach out to your local distributor.
Answered by: Tech Service
Date published: 2019-10-07

Can this antibody be used in immunohistochemistry for formalin fixed paraffin embedded samples?

Asked by: joannafin
Thank you for your question. We have not yet tested this monoclonal antibody, Pit-1 (D-7): sc-393943, for formalin-fixed paraffin-embedded tissue sections and so IHC(P) is not a recommended application at this time.
Answered by: Technical Support
Date published: 2017-09-20

Can this antibody be used to detect Pit-1 in chicken samples?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 5
Date published: 2017-01-19
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_393943, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 104ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from IF Works with mouse pituitaryImmuno-fluorescence staining worked with mouse pituitary sample (PFA fixed, Sucrose, cryosection)
Date published: 2018-10-10
Rated 5 out of 5 by from Works Great On FFPE TissueI bought this antibody 2 weeks ago and it looks great on FFPE tissue, strong intensity at a 1:80 dilution. Antigen retrieved in citrate buffer, using a polymer based detection.
Date published: 2017-10-30
Rated 5 out of 5 by from Strong WBVery nice western blot data; strong signal, low background
Date published: 2017-02-02
Rated 5 out of 5 by from Works Great!This antibody works great in western blot analysis of Pit-1 expression in rat pituitary tissue extract and GH3 whole cell lysate.
Date published: 2017-01-11
Rated 5 out of 5 by from IF in rat pituitaryIF in rat pituitary. Paraffin. TE recovery. 1: 300 dilution. 4 C overnight.
Date published: 2015-07-29
Rated 5 out of 5 by from Very good Western blot data of PitVery good Western blot data of Pit-1 expression in rat pituitary tissue extract and GH3 whole cell lysate . -SCBT QC
Date published: 2014-11-26
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_393943, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
Pit-1 抗体 (D-7) is rated 5.0 out of 5 by 6.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_393943, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 99ms
  • REVIEWS, PRODUCT