Date published: 2025-9-8

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Pin1 抗体 (G-8): sc-46660

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说明书
  • Pin1 抗体 G-8 是小鼠单克隆 IgG2a κ,Pin1抗体, 在72篇文献中引用,规格为200 µg/ml
  • 免疫human来源Pin1位于C-terminus 的41-163氨基酸
  • Pin1 抗体 (G-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Pin1
  • 抗Pin1抗体(G-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Pin1 (G-8): sc-46660免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 Pin1 抗体 (G-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Pin1 抗体 (G-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Pin1抗体(G-8)是一种小鼠单克隆IgG2a kappa轻链抗体,用于检测Pin1,也称为肽酰脯氨酸顺式/反式异构酶,NIMA相互作用1。抗Pin1抗体(G-8)识别人类Pin1的C端表位,包括氨基酸41-163,确保精确可靠的结合。Pin1是一种关键的酶,可催化磷酸化丝氨酸/苏氨酸-脯氨酸基序的顺式/反式异构化,这一过程对于蛋白质的正确折叠、功能和多种信号通路的调节至关重要。这种异构酶活性对于细胞周期进展至关重要,与包括癌症和神经退行性疾病在内的多种疾病有关,这凸显了其在正常细胞生理和病理条件下的重要性。Pin1单克隆抗体(G-8)对多种物种均具有广泛的反应性,可有效检测小鼠、大鼠和人类来源的Pin1,推荐用于蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)。这种多功能性使得 Pin1 (G-8) 抗体成为研究人员研究 Pin1 在细胞存活和疾病机制中的机制作用时不可或缺的工具。Pin1 (G-8) 单克隆抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和各种Alexa Fluor®结合物,可满足各种实验需求。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Pin1 抗体 (G-8) 参考文献:

  1. 哺乳动物双特异性蛋白激酶 Nek1 与 NIMA 细胞周期调节因子有关,在减数分裂生殖细胞中高度表达。  |  Letwin, K., et al. 1992. EMBO J. 11: 3521-31. PMID: 1382974
  2. 通过过量表达编码潜在蛋白激酶的 G2 特异性基因来诱导和维持有丝分裂。  |  Osmani, SA., et al. 1988. Cell. 53: 237-44. PMID: 3359487
  3. 与黑曲霉有丝分裂调节因子 NIMA 有关的新型人类蛋白激酶 Nek2 的细胞周期依赖性表达。  |  Schultz, SJ., et al. 1994. Cell Growth Differ. 5: 625-35. PMID: 7522034
  4. 一种对有丝分裂调节至关重要的人类肽基脯氨酰异构酶。  |  Lu, KP., et al. 1996. Nature. 380: 544-7. PMID: 8606777
  5. NIMA 相关激酶 2(Nek2)在减数分裂细胞周期的特定阶段表达,并与减数分裂染色体缔合作用。  |  Rhee, K. and Wolgemuth, DJ. 1997. Development. 124: 2167-77. PMID: 9187143
  6. 对有丝分裂旋转酶 Pin1 的结构和功能分析表明,底物识别依赖于磷酸化。  |  Ranganathan, R., et al. 1997. Cell. 89: 875-86. PMID: 9200606
  7. 哺乳动物 NIMA 相关激酶的特征。  |  Fry, AM. and Nigg, EA. 1997. Methods Enzymol. 283: 270-82. PMID: 9251026
  8. 序列特异性和磷酸化依赖性脯氨酸异构化:一种潜在的有丝分裂调控机制。  |  Yaffe, MB., et al. 1997. Science. 278: 1957-60. PMID: 9395400

订购信息

产品名称产品编号规格价格数量收藏夹

Pin1 抗体 (G-8)

sc-46660
200 µg/ml
RMB2377.00

Pin1 (G-8): m-IgG Fc BP-HRP 套装

sc-528492
200 µg Ab; 10 µg BP
RMB2662.00

Pin1 (G-8): m-IgGκ BP-HRP 套装

sc-520883
200 µg Ab, 40 µg BP
RMB2662.00

Pin1 (G-8): m-IgG2a BP-HRP 套装

sc-547107
200 µg Ab; 10 µg BP
RMB2662.00

Pin1 抗体 (G-8) AC

sc-46660 AC
500 µg/ml, 25% agarose
RMB3129.00

Pin1 抗体 (G-8) HRP

sc-46660 HRP
200 µg/ml
RMB2377.00

Pin1 抗体 (G-8) FITC

sc-46660 FITC
200 µg/ml
RMB2482.00

Pin1 抗体 (G-8) PE

sc-46660 PE
200 µg/ml
RMB2580.00

Pin1 抗体 (G-8) Alexa Fluor® 488

sc-46660 AF488
200 µg/ml
RMB2685.00

Pin1 抗体 (G-8) Alexa Fluor® 546

sc-46660 AF546
200 µg/ml
RMB2685.00

Pin1 抗体 (G-8) Alexa Fluor® 594

sc-46660 AF594
200 µg/ml
RMB2685.00

Pin1 抗体 (G-8) Alexa Fluor® 647

sc-46660 AF647
200 µg/ml
RMB2685.00

Pin1 抗体 (G-8) Alexa Fluor® 680

sc-46660 AF680
200 µg/ml
RMB2685.00

Pin1 抗体 (G-8) Alexa Fluor® 790

sc-46660 AF790
200 µg/ml
RMB2685.00

Can Pin1 Antibody (G-8): sc-46660 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Pin1 Antibody (G-8): sc-46660 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 5 out of 5 by from Works fineI have request a free sample of this antibody and it worked very well with my cell line (SH neuroblastoma) 1:500 works good.
Date published: 2017-10-09
Rated 5 out of 5 by from Used for WBTested at dilution 1:600 and HepG2 cell lysates (RIPA buffer) use.The bands was very clear with approriate MW.
Date published: 2017-08-19
Rated 5 out of 5 by from Great !!This antibody really working well. I have tested with mouse liver sample. It shows clear single band
Date published: 2017-07-26
Rated 5 out of 5 by from GOOD for WBI observed band with right size of Pin1 in WB, Dilution of primary antibody is 1: 100-500. Samples are whole cell lysates of HepG2 and PLC/PRF/5 Cells.
Date published: 2017-06-21
Rated 5 out of 5 by from wonderful antibody!I used this antibody for WB ,the protein was extracted form h9c2 cells.The result is satified.
Date published: 2017-04-10
Rated 5 out of 5 by from ExcellentThe antibody is very strong and specific when tested in Western blot
Date published: 2017-03-14
Rated 5 out of 5 by from Outstanding all applicationsVery strong clear western blot IHC and IF results !!
Date published: 2017-01-07
Rated 4 out of 5 by from Good anti-Pin1 antibody for IFSomeone in the lab next to ours used this antibody before. It looked like good for immunofluorescence.
Date published: 2016-12-29
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Pin1 抗体 (G-8) is rated 4.9 out of 5 by 17.
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