Date published: 2025-9-8

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PICK1 抗体 (F-10): sc-166654

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说明书
  • PICK1抗体(F-10)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 针对氨基酸1-300的抗原,在PICK1的N-terminus处映射,human来源
  • 推荐用于 mouse, rat 和 human 来源的PICK1 WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是PICK1 Antibody (F-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PICK1 Antibody (F-10) 打包提供(请参阅下面的订购信息)。

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与C激酶1(PICK1)相互作用的蛋白质是一种含有PDZ结构域的蛋白质,位于核周区域,并会在PKCα激活时发生磷酸化。PKCα对于调节多种细胞类型的增殖和分化至关重要,其催化区域内含有一个独特的PDZ结合域,该结构域在其他PKC亚型中是不存在的。PICK1的PDZ结构域发生突变会抑制PICK1与PKCα的结合。此外,PICK1还与α-氨基-3-羟基-5-甲基-异噁唑-4-丙酸(AMPA)受体的羧基末端相互作用,这是一种位于兴奋性突触的神经递质受体,这表明PICK1通过靶向和锚定AMPA到特定突触,在突触传递的调制中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PICK1 抗体 (F-10) 参考文献:

  1. PICK1-ICA69异源BAR结构域复合物调节突触定位和AMPA受体的表面表达。  |  Cao, M., et al. 2007. J Neurosci. 27: 12945-56. PMID: 18032668
  2. PICK1在皮质突触强化过程中调节钙渗透性AMPA受体的结合。  |  Clem, RL., et al. 2010. J Neurosci. 30: 6360-6. PMID: 20445062
  3. PICK1功能丧失会阻碍突触平衡调节。  |  Anggono, V., et al. 2011. J Neurosci. 31: 2188-96. PMID: 21307255
  4. PICK1的PDZ结构域与膜的结合和调节。  |  Erlendsson, S. and Madsen, KL. 2015. Membranes (Basel). 5: 597-615. PMID: 26501328
  5. PICK1/钙调神经磷酸酶抑制大鼠肺动脉平滑肌细胞中ASIC1介导的Ca2+进入。  |  Herbert, LM., et al. 2016. Am J Physiol Cell Physiol. 310: C390-400. PMID: 26702130
  6. PICK1对于胰岛素分泌和维持血糖平衡至关重要。  |  Li, J., et al. 2018. Mol Biol Cell. 29: 587-596. PMID: 29298842
  7. PICK1缺陷小鼠对可卡因的反应受损,多巴胺平衡失调。  |  Jensen, KL., et al. 2018. eNeuro. 5: PMID: 29911172
  8. PICK1依赖性细胞表面mGlu3受体的表达调节海马网络振荡。  |  Tuduri, P., et al. 2022. J Neurosci. 42: 8897-8911. PMID: 36202617
  9. 亚砷酸钠通过促进PICK1 BAR结构域同源二聚体的形成诱导聚合体的形成。  |  Lai, JHC., et al. 2024. Mol Biol Cell. 35: ar128. PMID: 39083353
  10. PICK1在螺旋线圈驱动的超分子复合物中连接KIBRA和AMPA受体亚基GluA2。  |  Shao, X. and Volk, L. 2025. J Biol Chem. 108397. PMID: 40074086

订购信息

产品名称产品编号规格价格数量收藏夹

PICK1 抗体 (F-10)

sc-166654
200 µg/ml
RMB2377.00

PICK1 (F-10): m-IgG Fc BP-HRP 套装

sc-537516
200 µg Ab; 10 µg BP
RMB2662.00

PICK1 (F-10): m-IgGκ BP-HRP 套装

sc-534803
200 µg Ab; 40 µg BP
RMB2662.00

PICK1 (F-10): m-IgG1 BP-HRP 套装

sc-545210
200 µg Ab; 20 µg BP
RMB2662.00

What is the recommended fixation method for immunofluorescence staining with PICK1 (F-10): sc-166654 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Beautiful immunofluorescence cytoplasmicBeautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Great Western Blot data of PICK1 expression in nonGreat Western Blot data of PICK1 expression in non-transfected and mouse PICK1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-10-18
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PICK1 抗体 (F-10) is rated 5.0 out of 5 by 2.
  • y_2025, m_8, d_30, h_9
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