Date published: 2025-9-8
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PI 3-kinase p110 Antibody (D-4) - Western Blotting - Image 2279
PI 3-kinase p110 抗体 (D-4): sc-8010. C32 全细胞裂解液中 PI 3- 激酶 p110 表达的 Western 印迹分析.
PI 3-kinase p110 Antibody (D-4) - Western Blotting - Image 2279
PI 3-kinase p110 抗体 (D-4): sc-8010. C32 全细胞裂解液中 PI 3- 激酶 p110 表达的 Western 印迹分析.
PI 3-kinase p110 Antibody (D-4) - Immunofluorescence - Image 370702
PI 3-kinase p110 抗体 (D-4): sc-8010. 福尔马林固定的 HeLa 细胞免疫荧光染色, 显示细胞质和细胞核定位.
PI 3-kinase p110 Antibody (D-4) - Western Blotting - Image 396167
用 PI 3-kinase p110 抗体 (D-4) Alexa Fluor® 680: sc-8010 AF680 检测 MG-63 全细胞裂解液中 PI 3-kinase p110 的表达, 用β-Actin (C4) Alexa Fluor® 790: sc-47778 AF790 检测β-Actin的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214.
PI 3-kinase p110 抗体 (D-4): sc-8010. C32 全细胞裂解液中 PI 3- 激酶 p110 表达的 Western 印迹分析.

PI 3-kinase p110 抗体 (D-4): sc-8010

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说明书
  • PI 3-kinase p110 抗体 D-4 是小鼠单克隆 IgG2a κ，PI 3-kinase p110抗体, 在43篇文献中引用，规格为200 µg/ml
  • 免疫human来源PI 3-kinase p110位于C-terminus 的800-1039氨基酸
  • PI 3-kinase p110 抗体 (D-4) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 PI 3-kinase p110
  • 抗PI 3-kinase p110抗体（D-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PI 3-kinase p110 (D-4): sc-8010的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是PI 3-kinase p110 Antibody (D-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PI 3-kinase p110 Antibody (D-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外PI 3-kinase抗体，包括PI 3-kinase C2 α, PI 3-kinase C2 β, PI 3-kinase C2 γ, PI 3-kinase p100, PI 3-kinase p101, PI 3-kinase p110, PI 3-kinase p110 α, PI 3-kinase p110 β, PI 3-kinase p110 δ, PI 3-kinase p110 γ, PI 3-kinase p55 γ, PI 3-kinase p85 α和PI 3-kinase p85 β

PI 3-激酶 p110 抗体（D-4）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测人类 PI 3-激酶 p110。PI 3-激酶 p110 (D-4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。磷脂酰肌醇3-激酶（PI 3-kinase）通路在细胞生长、增殖和存活等细胞功能中发挥着关键作用，因此研究PI 3-kinase的组成成分对于了解包括癌症在内的各种疾病至关重要。PI 3-激酶p110亚基负责酶的催化活性，将磷脂酰肌醇-4,5-二磷酸转化为磷脂酰肌醇-3,4,5-三磷酸，后者是激活下游通路的关键信号分子。该途径的调节至关重要，因为失调会导致细胞无序生长和肿瘤形成。此外，p110同工型具有不同的组织分布和调节机制，其中p110α和p110β广泛表达，而p110δ主要存在于白细胞中，这凸显了了解每个同工型在各种生物学环境中的特定作用的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PI 3-kinase p110 抗体 (D-4) 参考文献:

  1. 磷脂酰肌醇 3-激酶：110 kd 催化亚基的结构和表达。  |  Hiles, ID., et al. 1992. Cell. 70: 419-29. PMID: 1322797
  2. ANG II 可通过 PI3 激酶 p110 delta 通路增强全身高胰岛素血症糖尿病大鼠主动脉的收缩反应。  |  Kobayashi, T., et al. 2006. Am J Physiol Heart Circ Physiol. 291: H846-53. PMID: 16517941
  3. 与受体酪氨酸激酶, middle-T/pp60c-src 复合物和 PI3 激酶缔合作用的两种 85 kd 蛋白的特征。  |  Otsu, M., et al. 1991. Cell. 65: 91-104. PMID: 1707345
  4. 利用表达/克隆受体酪氨酸激酶靶蛋白的新方法克隆 PI3 激酶缔合作用 p85。  |  Skolnik, EY., et al. 1991. Cell. 65: 83-90. PMID: 1849461
  5. PI3-K 的 P110 亚基是阿卡西汀在皮肤癌中的治疗靶点。  |  Jung, SK., et al. 2014. Carcinogenesis. 35: 123-30. PMID: 23913940
  6. 克隆 G 蛋白激活的人类磷酸肌醇-3 激酶并确定其特性。  |  Stoyanov, B., et al. 1995. Science. 269: 690-3. PMID: 7624799
  7. 克隆一种新的, 普遍表达的人类磷脂酰肌醇 3- 激酶，并确定其在 p85 上的结合位点。  |  Hu, P., et al. 1993. Mol Cell Biol. 13: 7677-88. PMID: 8246984
  8. 截短的磷脂酰肌醇-3 激酶 p110 亚基的显性负效应。  |  Takayanagi, J., et al. 1996. Biochem Mol Biol Int. 39: 721-8. PMID: 8843340
  9. 白细胞中的新型磷酸肌醇 3- 激酶 P110delta  |  Vanhaesebroeck, B., et al. 1997. Proc Natl Acad Sci U S A. 94: 4330-5. PMID: 9113989
  10. Gbetagamma 通过与催化 p110 亚基的两个结构域直接相互作用，刺激磷酸肌醇 3-激酶-gamma。  |  Leopoldt, D., et al. 1998. J Biol Chem. 273: 7024-9. PMID: 9507010
  11. 在体外成纤维细胞衰老过程中，信号分子 Nck、c-Crk、Grb2/Ash、PI 3-kinase p110 alpha 和 WRN 的表达下调。  |  Matuoka, K. and Takenawa, T. 1998. Biochim Biophys Acta. 1401: 211-5. PMID: 9531977
  12. CD4 配体的结合会诱导磷脂酰肌醇-3 激酶 p110 亚基的酪氨酸磷酸化。  |  Mazerolles, F. and Fischer, A. 1998. Int Immunol. 10: 1897-905. PMID: 9885911

订购信息

产品名称产品编号规格价格数量收藏夹

PI 3-kinase p110 抗体 (D-4)

sc-8010
200 µg/ml
RMB2377.00

PI 3-kinase p110 (D-4): m-IgG Fc BP-HRP 套装

sc-528199
200 µg Ab; 10 µg BP
RMB2662.00

PI 3-kinase p110 (D-4): m-IgGκ BP-HRP 套装

sc-520521
200 µg Ab, 40 µg BP
RMB2662.00

PI 3-kinase p110 (D-4): m-IgG2a BP-HRP 套装

sc-547046
200 µg Ab; 10 µg BP
RMB2662.00

PI 3-kinase p110 抗体 (D-4) AC

sc-8010 AC
500 µg/ml, 25% agarose
RMB3129.00

PI 3-kinase p110 抗体 (D-4) HRP

sc-8010 HRP
200 µg/ml
RMB2377.00

PI 3-kinase p110 抗体 (D-4) FITC

sc-8010 FITC
200 µg/ml
RMB2482.00

PI 3-kinase p110 抗体 (D-4) PE

sc-8010 PE
200 µg/ml
RMB2580.00

PI 3-kinase p110 抗体 (D-4) Alexa Fluor® 488

sc-8010 AF488
200 µg/ml
RMB2685.00

PI 3-kinase p110 抗体 (D-4) Alexa Fluor® 546

sc-8010 AF546
200 µg/ml
RMB2685.00

PI 3-kinase p110 抗体 (D-4) Alexa Fluor® 594

sc-8010 AF594
200 µg/ml
RMB2685.00

PI 3-kinase p110 抗体 (D-4) Alexa Fluor® 647

sc-8010 AF647
200 µg/ml
RMB2685.00

PI 3-kinase p110 抗体 (D-4) Alexa Fluor® 680

sc-8010 AF680
200 µg/ml
RMB2685.00

PI 3-kinase p110 抗体 (D-4) Alexa Fluor® 790

sc-8010 AF790
200 µg/ml
RMB2685.00

这个p110的亚基是哪个呀

Asked by: nnFairy1212
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Does this recognise all alpha, beta and delta isoforms?

Asked by: Anatomical Science
Thank you for your question. This mouse monoclonal antibody was raised against PIK3CB, beta isoform; amino acids 800-1039 mapping at the C-terminus of PI 3-kinase p110 of human origin, protein accession number P42338. We have not evaluated the reactivity of this antibody with all isoforms.
Answered by: Technical Support
Date published: 2019-06-21

Tienen el fosforilado de proteína PI 3-kinase p110 ?

Asked by: FernandoGarzón
Gracias por tu pregunta. No ofrecemos un anticuerpo en contra de fosforilada PI 3-quinasa p110 en este momento. Thank you for your question. We do not offer a monoclonal antibody against phosphorylated PI 3-kinase p110 at this time.
Answered by: Technical Support
Date published: 2018-12-31

I probed for P110 gamma but nothing was detected. Dilution used was 1:1000 for 1 hr at room temperature.

Asked by: Srav
Thank you for your question. We recommend using this primary antibody at a starting dilution of 1:200 for Western blotting. Below is a link to our complete recommended protocol for Western blot. Please contact our Technical Service Department if you would like support in troubleshooting this antibody. Please call us toll-free at (800) 457-3801 option 2, or e-mail <[email protected]>. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-05-18

What is the recommended secondary antibody for Western blot when working with human samples?

Asked by: TinTin
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 14
Date published: 2017-02-04
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Rated 5 out of 5 by from Great productwe use this antibody for western blot. it works for us.
Date published: 2017-09-27
Rated 5 out of 5 by from The PI3K antibody is good for NCI-H1975The PI3K antibody is good for NCI-H1975 NSCLC cell line. The antibody is cost -efficient. I found it from many papers.
Date published: 2017-09-01
Rated 4 out of 5 by from good featureI used this antibody for WB, and it worek very well.
Date published: 2017-07-18
Rated 5 out of 5 by from greatI received this antibody a month ago.I used it for western blot.The results were great.
Date published: 2017-07-18
Rated 5 out of 5 by from Popular choice!This is a popular choice for detection of PI 3-kinase p110 in Western blotting and immunoprecipitation applications as indicated by the over 15 product citations.
Date published: 2017-01-18
Rated 5 out of 5 by from great antibodyThis antibody has great specificity .Clean band can be observed in Western Blot analysis of PI 3-kinase expression in HeLa cell protein.
Date published: 2017-01-18
Rated 5 out of 5 by from Correct MW band in human MGCorrect MW band in human MG-63 cells. -SCBT Publication Review
Date published: 2015-04-06
Rated 5 out of 5 by from Worked well in IP and WB in human PCWorked well in IP and WB in human PC-3, LNCaP and DU145 cell lines. -SCBT Publication Review
Date published: 2015-03-18
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PI 3-kinase p110 抗体 (D-4) is rated 4.9 out of 5 by 12.
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