Date published: 2025-10-25
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Phox2b Antibody (B-11) - Western Blotting - Image 283861
Phox2b 抗体 (B-11): sc-376997. SK-N-SH (A), IMR-32 (B) 和 Neuro-2a (C) 全细胞裂解液中 Phox2b 表达的 Western 印迹分析.
Phox2b 抗体 (B-11): sc-376997. SK-N-SH (A), IMR-32 (B) 和 Neuro-2a (C) 全细胞裂解液中 Phox2b 表达的 Western 印迹分析.

Phox2b 抗体 (B-11): sc-376997

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说明书
  • Phox2b 抗体 B-11 是小鼠单克隆 IgG1 κ，Phox2b抗体, 在41篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列11-70位于human物种的Phox2b的N-terminus附近
  • Phox2b 抗体 (B-11) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Phox2b; 也和以下物种反应，包括： 和 equine, canine and bovine
  • 抗Phox2b抗体（B-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-376997 X, 200 µg/0.1 ml)
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Phox2b Antibody (B-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Phox2b Antibody (B-11) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外Phox抗体，包括Phox2b

Phox2b抗体（B-11）是一种IgG1 κ小鼠单克隆Phox2b抗体（也称为PHX2B抗体、成对样同源框2B抗体、NBPhox抗体、成对中胚层同源框2b抗体、PHOX2B同源域蛋白抗体、神经母细胞瘤Phox抗体、成对样同源框2B抗体、成对样同源框2B抗体或NBLST2抗体），它通过WB、IP、IF和ELISA检测来自小鼠、大鼠和人的Phox2b蛋白。Phox2b抗体（B-11）既有非偶联形式的抗Phox2b抗体，也有多种偶联形式的抗Phox2b抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Phox2a（也称为Arix1）和Phox2b是密切相关的成对同源结构域转录因子，对于发育中的交感神经节、副交感神经节和肠神经节的神经元分化都是必需的。所有肠神经系统细胞都是从神经嵴演化而来的，所有最初未分化的细胞都表达Phox2b。开始沿神经元谱系分化的细胞继续表达Phox2b，并开始表达Phox2a。Phox2b是大脑中所有中枢和非外周去甲肾上腺素能中心分化所必需的。相比之下，Phox2a仅控制大脑中去甲肾上腺素能中心的主要部分，即蓝斑的分化。Phox2a和Phox2b对于调节内源性酪氨酸羟化酶和多巴胺-β羟化酶都是至关重要的，这些酶在神经嵴细胞中瞬时表达。此外，Phox2蛋白足以促进交感神经元的生成。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Phox2b 抗体 (B-11) 参考文献:

  1. 神经嵴干细胞中的 Phox2 蛋白对神经递质特性的指定  |  Lo, L., et al. 1999. Neuron. 22: 693-705. PMID: 10230790
  2. 同源基因 Phox2b 对自主神经嵴衍生物的发育至关重要。  |  Pattyn, A., et al. 1999. Nature. 399: 366-70. PMID: 10360575
  3. Phox2 同源域蛋白足以促进交感神经元的发育。  |  Stanke, M., et al. 1999. Development. 126: 4087-94. PMID: 10457017
  4. 胚胎小鼠肠道中未分化的神经嵴衍生细胞和不同类别的肠道神经元表达 Ret, p75(NTR), Phox2a, Phox2b 和酪氨酸羟化酶免疫反应。  |  Young, HM., et al. 1999. Dev Dyn. 216: 137-52. PMID: 10536054
  5. 同源基因 Phox2b 对中枢去甲肾上腺素能表型的规定  |  Pattyn, A., et al. 2000. Mol Cell Neurosci. 15: 235-43. PMID: 10736201
  6. 神经母细胞瘤中配对样同源框2B（PHOX2B）基因的种系突变。  |  Bourdeaut, F., et al. 2005. Cancer Lett. 228: 51-8. PMID: 15949893
  7. 表达化学敏感性 Phox2b 的神经元对脊髓束核的高碳酸血症通气反应至关重要。  |  Fu, C., et al. 2017. J Physiol. 595: 4973-4989. PMID: 28488367
  8. Phox2b免疫组织化学染色在检测先天性巨结肠病中的肠神经嵴细胞中的应用。  |  Alturkustani, M., et al. 2021. Pediatr Dev Pathol. 24: 19-26. PMID: 32975470
  9. PHOX2B稳定性降低会导致TARDBP突变型运动神经元的轴突生长受损。  |  Mitsuzawa, S., et al. 2021. Stem Cell Reports. 16: 1527-1541. PMID: 34048688
  10. 在皮特-霍普金斯综合征模型中，呼吸紊乱涉及表达Phox2b的副面神经元和异常的Nav1.8表达。  |  Cleary, CM., et al. 2021. Nat Commun. 12: 5962. PMID: 34645823
  11. 大鼠喙小细胞网状结构中表达Phox2b的神经元的内在特性和突触连接。  |  Kajiwara, R., et al. 2022. Neurosci Res. 178: 41-51. PMID: 34973291
  12. 将 ARIX 同源域基因绘制到小鼠 7 号染色体和人类 11q13 号染色体上。  |  Johnson, KR., et al. 1996. Genomics. 33: 527-31. PMID: 8661014

订购信息

产品名称产品编号规格价格数量收藏夹

Phox2b 抗体 (B-11)

sc-376997
200 µg/ml
RMB2377.00

Phox2b (B-11): m-IgGκ BP-HRP 套装

sc-523145
200 µg Ab, 40 µg BP
RMB2662.00

Phox2b (B-11): m-IgG1 BP-HRP 套装

sc-533557
200 µg Ab; 20 µg BP
RMB2662.00

Phox2b 抗体 (B-11) X

sc-376997 X
200 µg/0.1 ml
RMB2377.00

Phox2b 抗体 (B-11) AC

sc-376997 AC
500 µg/ml, 25% agarose
RMB3129.00

Phox2b 抗体 (B-11) HRP

sc-376997 HRP
200 µg/ml
RMB2377.00

Phox2b 抗体 (B-11) FITC

sc-376997 FITC
200 µg/ml
RMB2482.00

Phox2b 抗体 (B-11) PE

sc-376997 PE
200 µg/ml
RMB2580.00

Phox2b 抗体 (B-11) Alexa Fluor® 488

sc-376997 AF488
200 µg/ml
RMB2685.00

Phox2b 抗体 (B-11) Alexa Fluor® 546

sc-376997 AF546
200 µg/ml
RMB2685.00

Phox2b 抗体 (B-11) Alexa Fluor® 594

sc-376997 AF594
200 µg/ml
RMB2685.00

Phox2b 抗体 (B-11) Alexa Fluor® 647

sc-376997 AF647
200 µg/ml
RMB2685.00

Phox2b 抗体 (B-11) Alexa Fluor® 680

sc-376997 AF680
200 µg/ml
RMB2685.00

Phox2b 抗体 (B-11) Alexa Fluor® 790

sc-376997 AF790
200 µg/ml
RMB2685.00

do you have a mouse anti-Phox2b primary antibody which is good for IHC (P) work? Thank you very much!

Asked by: Anonymous
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2022-08-17

I'd like to do IHC (P) experiment using the mouse anti-Phox2b. Should I use sc-376997 or sc-376997 X? What is the difference between the to abs and which is better for IHC(P)? Can I store the aliquots of mouse anti-phox2b antibody in -20 freezer?

Asked by: Anonymous
Thank you for your question. The regular antibody is supplied as 200 µg in 1 ml PBS with gelatin and sodium azide. The X-formulation contains no gelatin and comes as 200 µg in 0.1 ml. Neither would be recommended for IHC(P) applications. The antibody should always be kept at +4 °C for storage.
Answered by: Tech Support Europe
Date published: 2022-08-15

When should the TransCruz reagent sc-376997 X be used instead of the standard form sc-376997?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Santa Cruz TS
Date published: 2017-01-27
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Rated 2 out of 5 by from Ok labelling but weak intensityIt labels the Phox2b+ cells in immunofluorescence but there are better Phox2b antibodies - clearly an inferior product
Date published: 2023-06-20
Rated 5 out of 5 by from Clear band was detectedProtein lysates from Neuroblastoma cells SH-SY5Y (PHOX2B negative) and SH-EP (PHOX2B positive) were loaded, and PHOX2B antibody (B-11) was tested in different antibody diluents by western blotting.
Date published: 2019-01-26
Rated 5 out of 5 by from Very specific antibody for immunoI bought this antibody to test in whole mount immunohistochemistry. I works very well on my zebrafish embryos.
Date published: 2018-07-03
Rated 5 out of 5 by from B-11 works well in zebrafish embryoI requested a free sample of Phox2b antibody B-11 from Santa Cruz Biotechnology Inc. one month ago, and performed whole mount immunohistochemistry in zebrafish embryos recently. We got high qualify images with this antibody. The service of Santa Cruz Biotechnology Inc. is excellent, too! Thank you so much!
Date published: 2017-08-24
Rated 5 out of 5 by from Expression detected in rat brainThis product gives reasonable expression following standard IHC protocol for brain slices.
Date published: 2017-05-11
Rated 5 out of 5 by from Correct choice for Phox2b detectionThis antibody gives you the expected band in WB, clean blots.
Date published: 2016-12-21
Rated 5 out of 5 by from il marche trop bien !facile à utiliser, en immunohistochimie, chez le poisson zèbre
Date published: 2016-12-19
Rated 4 out of 5 by from Strong bands seen in WB using SKStrong bands seen in WB using SK-N-SH, IMR-32 and Neuro-2a whole cell lysates. -SCBT QC
Date published: 2015-02-01
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Phox2b 抗体 (B-11) is rated 4.6 out of 5 by 9.
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