Date published: 2025-9-7
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PGDH Antibody (H-3) - Western Blotting - Image 358674
PGDH 抗体 (H-3): sc-271418. 人胎盘 (A) 和 Caco-2 (B) 全细胞裂解液中 PGDH 表达的 Western 印迹分析.
PGDH 抗体 (H-3): sc-271418. 人胎盘 (A) 和 Caco-2 (B) 全细胞裂解液中 PGDH 表达的 Western 印迹分析.

PGDH 抗体 (H-3): sc-271418

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说明书
  • PGDH 抗体 H-3 是小鼠单克隆 IgG1 κ，PGDH抗体, 在3篇文献中引用，规格为200 µg/ml
  • 免疫human物种的PGDH的氨基酸1-263
  • PGDH 抗体 (H-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 PGDH
  • 抗PGDH抗体（H-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PGDH (H-3): sc-271418的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是PGDH Antibody (H-3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PGDH Antibody (H-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

PGDH抗体（H-3）是一种IgG1 κ小鼠单克隆PGDH抗体（也称为PGDH抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的PGDH蛋白。PGDH抗体（H-3）既有非偶联形式的抗PGDH抗体，也有多种偶联形式的抗PGDH抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。前列腺素涉及许多生理和细胞过程，如炎症。NAD+-依赖的15-羟基前列腺素脱氢酶（PGDH）是前列腺素降解的基本酶。PGDH是一种普遍存在的酶，通过催化前列腺素15-羟基氧化为酮基，强烈降低这些分子的生物活性。皮质醇可降低人胎盘细胞中的PGDH活性。11-β-羟基类固醇脱氢酶II型（HSD11B2）将皮质醇转化为皮质酮。子痫前期是一种以怀孕期间和产后高血压和蛋白尿为特征的疾病，HSD11B2 mRNA表达减少，导致HSD11B2活性降低。因此，胎盘皮质醇转化减少可能通过自分泌或旁分泌机制导致PGDH mRNA表达减少。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PGDH 抗体 (H-3) 参考文献:

  1. 膀胱过渡性和鳞状细胞癌中细胞角蛋白 20, AN43, PGDH 和 COX-2 的表达。  |  Gee, JR., et al. 2003. Urol Oncol. 21: 266-70. PMID: 12954496
  2. 与 NAD+ 链接的 15-hydroxyprostaglandin dehydrogenase (15-PGDH) 在肺癌中具有肿瘤抑制作用。  |  Ding, Y., et al. 2005. Carcinogenesis. 26: 65-72. PMID: 15358636
  3. 妊娠晚期和分娩期人类绒毛膜中 15-hydroxyprostaglandin dehydrogenase (PGDH) 基因活性, 信使核糖核酸处理和蛋白质丰度的调节。  |  Johnson, RF., et al. 2004. J Clin Endocrinol Metab. 89: 5639-48. PMID: 15531523
  4. 15-羟基前列腺素脱氢酶在结直肠癌中下调。  |  Backlund, MG., et al. 2005. J Biol Chem. 280: 3217-23. PMID: 15542609
  5. 15-羟基前列腺素脱氢酶是一种 COX-2 致癌基因拮抗剂，是一种由 TGF-beta 诱导的人类胃肠道癌症抑制剂。  |  Yan, M., et al. 2004. Proc Natl Acad Sci U S A. 101: 17468-73. PMID: 15574495
  6. 人类 15-羟基前列腺素脱氢酶中关键的 NAD+ 结合残基。  |  Cho, H., et al. 2005. Arch Biochem Biophys. 433: 447-53. PMID: 15581601
  7. 地塞米松和其他糖皮质激素可在治疗水平上诱导 A549 人肺腺癌细胞中的 15-羟基前列腺素脱氢酶。  |  Tong, M. and Tai, HH. 2005. Arch Biochem Biophys. 435: 50-5. PMID: 15680906
  8. 通过同源建模, 对接和分子动力学模拟了解人类 15-hydroxyprostaglandin dehydrogenase 与 NAD+ 和 PGE2 的结合。  |  Hamza, A., et al. 2005. Bioorg Med Chem. 13: 4544-51. PMID: 15908215
  9. 抑制前列腺素降解酶 15-PGDH 可使老年肌肉质量和力量恢复青春活力。  |  Palla, AR., et al. 2021. Science. 371: PMID: 33303683
  10. 通过脂肪酸诱导的 T 细胞衰竭，下调 15-PGDH 可促进 MASH-HCC 的发展。  |  Hu, X., et al. 2023. JHEP Rep. 5: 100892. PMID: 37942226
  11. 马胎盘中 15-羟基前列腺素脱氢酶 (PGDH) 和类固醇生成酶的定位。  |  Han, X., et al. 1995. Equine Vet J. 27: 334-9. PMID: 8654347
  12. 感染时，早产患者胎膜中的免疫活性 15-羟基前列腺素脱氢酶（PGDH）会降低。  |  Van Meir, CA., et al. 1996. Placenta. 17: 291-7. PMID: 8829211

订购信息

产品名称产品编号规格价格数量收藏夹

PGDH 抗体 (H-3)

sc-271418
200 µg/ml
RMB2377.00

PGDH (H-3): m-IgG Fc BP-HRP 套装

sc-529181
200 µg Ab; 10 µg BP
RMB2662.00

PGDH (H-3): m-IgGκ BP-HRP 套装

sc-521939
200 µg Ab, 40 µg BP
RMB2662.00

PGDH (H-3): m-IgG1 BP-HRP 套装

sc-543374
200 µg Ab; 20 µg BP
RMB2662.00

PGDH 抗体 (H-3) AC

sc-271418 AC
500 µg/ml, 25% agarose
RMB3129.00

PGDH 抗体 (H-3) HRP

sc-271418 HRP
200 µg/ml
RMB2377.00

PGDH 抗体 (H-3) FITC

sc-271418 FITC
200 µg/ml
RMB2482.00

PGDH 抗体 (H-3) PE

sc-271418 PE
200 µg/ml
RMB2580.00

PGDH 抗体 (H-3) Alexa Fluor® 488

sc-271418 AF488
200 µg/ml
RMB2685.00

PGDH 抗体 (H-3) Alexa Fluor® 546

sc-271418 AF546
200 µg/ml
RMB2685.00

PGDH 抗体 (H-3) Alexa Fluor® 594

sc-271418 AF594
200 µg/ml
RMB2685.00

PGDH 抗体 (H-3) Alexa Fluor® 647

sc-271418 AF647
200 µg/ml
RMB2685.00

PGDH 抗体 (H-3) Alexa Fluor® 680

sc-271418 AF680
200 µg/ml
RMB2685.00

PGDH 抗体 (H-3) Alexa Fluor® 790

sc-271418 AF790
200 µg/ml
RMB2685.00

I have an experiment on 15-Hydroxy Prostaglandin Dehydrogenase in Chicken, which antibody is suitable for ELISA or western blot or IHC?

Asked by: Moustafa
Thank you for your question. This antibody has not been tested on chicken samples, so we can not say for sure if it will work or not. It is recommended for ELISA, WB and IHC, so all those applications should be fine on recommended samples. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-07

I am using PGDH (H-3): sc-271418 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from used for WBThe antibody is fist time used.But tried only one time got spcific band, I tried dilution 1:800.
Date published: 2017-06-11
Rated 5 out of 5 by from specific bandStrong specific band seen in WB with mouse PGDH transfected lysates.
Date published: 2017-05-28
Rated 5 out of 5 by from Robust signalRobust signal in WB in human transfected 293T and Jurkat whole cell lysates.
Date published: 2017-02-23
Rated 5 out of 5 by from Strong and reliable for IHC and WBExcellent choice if you are studying PGDH: clean and specific results in both IHC and WB.
Date published: 2017-01-25
Rated 5 out of 5 by from Worked great in WB with primary mouse macrophagesWorked great in WB with primary mouse macrophages (Dil 1:400)
Date published: 2016-07-28
Rated 5 out of 5 by from Produced positive Western Blot data of PGDHProduced positive Western Blot data of PGDH expression in non-transfected and mouse PSPC1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-04-15
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PGDH抗体 (H-3) is rated 5.0 out of 5 by 6.
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