Date published: 2025-9-7
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PEPCK/PCK1/PCK2 Antibody (F-3) - Western Blotting - Image 377810
PEPCK 抗体 (F-3): sc-271029. A-431 (A), ZR-75-1 (B), Caki-1 (C), NIH/3T3 (D) 和 c4 (E) 全细胞裂解液及大鼠肝组织提取物 (F) 中 PEPCK 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 790: sc-516181.
PEPCK 抗体 (F-3): sc-271029. A-431 (A), ZR-75-1 (B), Caki-1 (C), NIH/3T3 (D) 和 c4 (E) 全细胞裂解液及大鼠肝组织提取物 (F) 中 PEPCK 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 790: sc-516181.

PEPCK/PCK1/PCK2 抗体 (F-3): sc-271029

4.8(9)
寫評論提問

说明书
  • PEPCK/PCK1/PCK2 抗体 F-3 是小鼠单克隆 IgG2b κ，PEPCK/PCK1/PCK2抗体, 在32篇文献中引用，规格为200 µg/ml
  • 针对氨基酸341-640的抗原，在PEPCK-M的C-terminus处映射，human来源
  • PEPCK/PCK1/PCK2 抗体 (F-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PEPCK-M and PEPCK-C
  • 抗PEPCK/PCK1/PCK2抗体（F-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PEPCK/PCK1/PCK2 (F-3): sc-271029的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 PEPCK/PCK1/PCK2 抗体 (F-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PEPCK/PCK1/PCK2 抗体 (F-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加PEPCK抗体，包括PEPCK-C和PEPCK-M

PEPCK抗体（F-3）是一种IgG2b κ鼠单克隆PEPCK抗体（也称为PCK1/2抗体或PEPCK-C/M抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源性的PEPCK蛋白。PEPCK抗体（F-3）既可以是非偶联的抗PEPCK抗体形式，也可以是多种偶联形式的抗PEPCK抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。血糖水平的正常调节依赖于胰岛素信号传导以及参与糖异生调节的酶。该过程的病理变化是2型糖尿病表型的核心。磷酸烯醇丙酮酸羧激酶（PEPCK）通过刺激肝脏葡萄糖产生在这一过程中发挥重要作用。PEPCK的表达会增加以响应胰高血糖素和糖皮质激素，而胰岛素则会抑制其表达。调节控制PEPCK水平的信号为治疗胰岛素抵抗以及随后的肥胖症提供了一种潜在的治疗方法。PEPCK的细胞质形式，称为PEPCK-C，以及其线粒体形式，称为PEPCK-M，在小鼠、人类和鸡中由两个不同的核基因编码。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PEPCK/PCK1/PCK2 抗体 (F-3) 参考文献:

  1. [类固醇糖尿病机制的新方面以及胰岛素和类固醇对肝糖生成的调节]。  |  Barthel, A., et al. 2003. Med Klin (Munich). 98: 283-6. PMID: 12721674
  2. 在日本 2 型糖尿病患者中发现磷酸烯醇丙酮酸羧激酶基因启动子的新型变异。  |  Horikawa, Y., et al. 2003. Horm Metab Res. 35: 308-12. PMID: 12916001
  3. 胰岛素调节肝糖生成的新概念。  |  Barthel, A. and Schmoll, D. 2003. Am J Physiol Endocrinol Metab. 285: E685-92. PMID: 12959935
  4. 转基因脂肪连通素的持续外周表达可抵消饮食诱发大鼠肥胖症的发展。  |  Shklyaev, S., et al. 2003. Proc Natl Acad Sci U S A. 100: 14217-22. PMID: 14617771
  5. AMP激活的蛋白激酶通过直接磷酸化新型锌指转录因子来调节PEPCK基因的表达。  |  Inoue, E. and Yamauchi, J. 2006. Biochem Biophys Res Commun. 351: 793-9. PMID: 17097062
  6. 胰岛素和其他调节因子可调节无血清培养基中原代兔肾近曲小管细胞的生长和磷酸烯醇丙酮酸羧激酶（PEPCK）的活性。  |  Wang, Y. and Taub, M. 1991. J Cell Physiol. 147: 374-82. PMID: 1710231
  7. 大鼠细胞质 PEPCK 的结构：深入了解草酰乙酸磷酸化和脱羧反应的机制。  |  Sullivan, SM. and Holyoak, T. 2007. Biochemistry. 46: 10078-88. PMID: 17685635
  8. 鉴定 PEPCK 基因中介导胰岛素对转录产生负面影响的序列。  |  O'Brien, RM., et al. 1990. Science. 249: 533-7. PMID: 2166335
  9. 胰岛素通过一种不涉及 cAMP 的机制降低 H4IIE 细胞 PEPCK mRNA。  |  Beale, EG., et al. 1986. Diabetes. 35: 546-9. PMID: 3007246

订购信息

产品名称产品编号规格价格数量收藏夹

PEPCK/PCK1/PCK2 抗体 (F-3)

sc-271029
200 µg/ml
RMB2377.00

PEPCK/PCK1/PCK2 (F-3): m-IgGκ BP-HRP 套装

sc-521820
200 µg Ab, 40 µg BP
RMB2662.00

PEPCK/PCK1/PCK2 (F-3): m-IgG2b BP-HRP 套装

sc-550935
200 µg Ab; 10 µg BP
RMB2662.00

PEPCK/PCK1/PCK2 抗体 (F-3) AC

sc-271029 AC
500 µg/ml, 25% agarose
RMB3129.00

PEPCK/PCK1/PCK2 抗体 (F-3) HRP

sc-271029 HRP
200 µg/ml
RMB2377.00

PEPCK/PCK1/PCK2 抗体 (F-3) FITC

sc-271029 FITC
200 µg/ml
RMB2482.00

PEPCK/PCK1/PCK2 抗体 (F-3) PE

sc-271029 PE
200 µg/ml
RMB2580.00

PEPCK/PCK1/PCK2 抗体 (F-3) Alexa Fluor® 488

sc-271029 AF488
200 µg/ml
RMB2685.00

PEPCK/PCK1/PCK2 抗体 (F-3) Alexa Fluor® 546

sc-271029 AF546
200 µg/ml
RMB2685.00

PEPCK/PCK1/PCK2 抗体 (F-3) Alexa Fluor® 594

sc-271029 AF594
200 µg/ml
RMB2685.00

PEPCK/PCK1/PCK2 抗体 (F-3) Alexa Fluor® 647

sc-271029 AF647
200 µg/ml
RMB2685.00

PEPCK/PCK1/PCK2 抗体 (F-3) Alexa Fluor® 680

sc-271029 AF680
200 µg/ml
RMB2685.00

PEPCK/PCK1/PCK2 抗体 (F-3) Alexa Fluor® 790

sc-271029 AF790
200 µg/ml
RMB2685.00

Can PEPCK (F-3): sc-271029 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PEPCK (F-3): sc-271029 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from IHC in human tissuesGood staining for IHC in breast cancer and normal breast tissues
Date published: 2023-01-10
Rated 5 out of 5 by from excellnt antibodyI used this antibody for WB in mouse liver extracts. Very strong signals were detected.
Date published: 2022-03-11
Rated 5 out of 5 by from Work well in western blotTested this antibody with AML12 cell line total protein. At 1:1000 dilution, band was right on the estimated size with clean background when exposed with ECL, and visualized using chemidoc instrument.
Date published: 2017-12-13
Rated 5 out of 5 by from Very good antibodyVery strong signal detected, I would recommend to use.
Date published: 2017-08-24
Rated 5 out of 5 by from Great choice of antibody!This is a very strong antibody, especially for Western blot. Easy to work with, nice strong signal.
Date published: 2017-01-30
Rated 5 out of 5 by from WBRight size WB band exists in human macrophage sapmles, and the expression pattern in different kinds of macrophages is as expected.
Date published: 2016-12-24
Rated 5 out of 5 by from Detection of PEPCK of mouse and ratDetection of PEPCK of mouse and rat.
Date published: 2015-10-02
Rated 5 out of 5 by from Superb immunofluorescence mitochondrial stainingSuperb immunofluorescence mitochondrial staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-22
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PEPCK/PCK1/PCK2 抗体 (F-3) is rated 4.8 out of 5 by 9.
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