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PEA3 Antibody (16) - Western Blotting - Image 887
PEA3 抗体 (16): sc-113. K-562 (A) 和 KNRK (B) 核提取物中 PEA3 表达的 Western 印迹分析.
PEA3 Antibody (16) - Western Blotting - Image 887
PEA3 抗体 (16): sc-113. K-562 (A) 和 KNRK (B) 核提取物中 PEA3 表达的 Western 印迹分析.
PEA3 抗体 (16): sc-113. K-562 (A) 和 KNRK (B) 核提取物中 PEA3 表达的 Western 印迹分析.

PEA3 抗体 (16): sc-113

4.8(14)
寫評論提問

说明书
  • PEA3抗体(16)是小鼠单克隆IgG1 κ, 在76篇文献中引用，规格为200 µg/ml
  • 针对重组PEA3融合蛋白
  • 抗-PEA3 抗体 (16) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 PEA3 p60
  • 抗PEA3抗体（16）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-113 X, 200 µg/0.1 ml)
  • 关于如何获取PEA3 (16): sc-113的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是PEA3 Antibody (16) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PEA3 Antibody (16) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外PEA抗体，包括PEA3

PEA3 抗体（16）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测小鼠、大鼠和人源的 PEA3 p60 蛋白。PEA3 (16) 单克隆抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。PEA3蛋白是Ets基因家族的一员，通过与特定DNA序列结合来调节基因表达，在包括发育和分化在内的各种细胞过程中发挥着至关重要的作用。PEA3能够识别5′-AGGAAG-3′的PEA-3序列，而PU.1等其他Ets家族成员则与类似但不同的序列结合。这种DNA结合的特异性使得PEA3能够调节上皮和成纤维细胞功能的关键靶基因，而PEA3在造血细胞中明显缺乏表达，这使其有别于其他Ets蛋白，如Ets-1和Ets-2。PEA3的独特表达模式凸显了其在组织特异性基因调控中的重要性，并凸显了其在发育生物学和癌症研究中作为研究目标的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PEA3 抗体 (16) 参考文献:

  1. PEA3的分子克隆和特征描述，PEA3是Ets癌基因家族的一个新成员，在小鼠胚胎细胞中有不同表达。  |  Xin, JH., et al. 1992. Genes Dev. 6: 481-96. PMID: 1547944
  2. Ets-1转录因子是前T细胞受体完整功能和T细胞受体β位点等位基因排斥所必需的。  |  Eyquem, S., et al. 2004. Proc Natl Acad Sci U S A. 101: 15712-7. PMID: 15496469
  3. 果蝇 74EF 早期浮肿包含 E74，这是一个复杂的蜕皮激素诱导基因，编码两种与蜕皮激素相关的蛋白质。  |  Burtis, KC., et al. 1990. Cell. 61: 85-99. PMID: 2107982
  4. 麋鹿，X 和 14 号染色体上与组织特异性等位基因相关的基因，靠近易位断点。  |  Rao, VN., et al. 1989. Science. 244: 66-70. PMID: 2539641
  5. c-ets 癌基因编码的 54,000 细胞质蛋白在胸腺和法氏囊淋巴细胞中的鉴定和优先表达。  |  Ghysdael, J., et al. 1986. Proc Natl Acad Sci U S A. 83: 1714-8. PMID: 3006066
  6. 21 号染色体上的人类 ets 相关基因 erg：替代剪接, 多腺苷酸化和翻译。  |  Rao, VN., et al. 1987. Science. 237: 635-9. PMID: 3299708
  7. ETS 转录因子 PEA3 亚家族在癌症中的功能和调控。  |  Qi, T., et al. 2020. Am J Cancer Res. 10: 3083-3105. PMID: 33163259
  8. 牙源性囊肿和肿瘤中 PEA3 的差异表达。  |  Tun, KM., et al. 2023. J Oral Pathol Med. 52: 777-785. PMID: 37549030
  9. Pea3转录因子亚家族在神经系统中的作用  |  Kandemir, B. and Kurnaz, IA. 2025. Mol Neurobiol. 62: 3293-3304. PMID: 39269548
  10. Ets1 转录因子在小鼠胚胎发育过程中广泛表达，与参与器官形成等形态发生过程的中胚层细胞有关。  |  Kola, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 7588-92. PMID: 7689222
  11. PU.1 磷酸化对与 NF-EM5 的相互作用和转录激活的影响。  |  Pongubala, JM., et al. 1993. Science. 259: 1622-5. PMID: 8456286
  12. 11号染色体长臂部分缺失[del(11)(q24.2qter)]和慢性血小板减少性紫癜患者体内的微小核细胞。  |  Gangarossa, S., et al. 1996. Am J Med Genet. 62: 120-3. PMID: 8882392

订购信息

产品名称产品编号规格价格数量收藏夹

PEA3 抗体 (16)

sc-113
200 µg/ml
RMB2377.00

PEA3 (16): m-IgG Fc BP-HRP 套装

sc-528098
200 µg Ab; 10 µg BP
RMB2662.00

PEA3 (16): m-IgGκ BP-HRP 套装

sc-520396
200 µg Ab, 40 µg BP
RMB2662.00

PEA3 (16): m-IgG1 BP-HRP 套装

sc-542757
200 µg Ab; 20 µg BP
RMB2662.00

PEA3 抗体 (16) X

sc-113 X
200 µg/0.1 ml
RMB2377.00

PEA3 抗体 (16) AC

sc-113 AC
500 µg/ml, 25% agarose
RMB3129.00

PEA3 抗体 (16) HRP

sc-113 HRP
200 µg/ml
RMB2377.00

PEA3 抗体 (16) FITC

sc-113 FITC
200 µg/ml
RMB2482.00

PEA3 抗体 (16) PE

sc-113 PE
200 µg/ml
RMB2580.00

PEA3 抗体 (16) Alexa Fluor® 488

sc-113 AF488
200 µg/ml
RMB2685.00

PEA3 抗体 (16) Alexa Fluor® 546

sc-113 AF546
200 µg/ml
RMB2685.00

PEA3 抗体 (16) Alexa Fluor® 594

sc-113 AF594
200 µg/ml
RMB2685.00

PEA3 抗体 (16) Alexa Fluor® 647

sc-113 AF647
200 µg/ml
RMB2685.00

PEA3 抗体 (16) Alexa Fluor® 680

sc-113 AF680
200 µg/ml
RMB2685.00

PEA3 抗体 (16) Alexa Fluor® 790

sc-113 AF790
200 µg/ml
RMB2685.00

请问这一款与sc-166629有什么区别

Asked by: Jiner
Thanks for your questions. Anti-PEA3 Antibody (G-10) is recommended for detection of PEA3 of mouse, rat and human origin by WB, IP, IF and ELISA. Anti-PEA3 Antibody (16) is recommended for detection of PEA3 p60 of mouse, rat and human origin by WB, IP and IF. 感谢您的提问。Anti-PEA3 Antibody (G-10) 和 Anti-PEA3 Antibody (16)推荐的检测实验方法范围不同。
Answered by: Yanru Tao
Date published: 2022-09-05

说明书为什么下载不了，sc-113

Asked by: 11111
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-09-07

Our WB (PEA3 Antibody: sc-113) analysis of the PEA3 expression in H2122 cell showed that the band is located at a molecular weight below 50 KD, which, however, is inconsistent with the one indicated in the datasheet. Is this the right band? Thanks.

Asked by: Haitang
Thank you for your question. The expected molecular weight of PEA3 as detected by PEA3 Antibody (16): sc-113 is 62 kDa. If you are detecting a signal on a different height please contact our technical service for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

When should the TransCruz reagent sc-113 X be used instead of the standard form sc-113?

Asked by: jenni
We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 5 out of 5 by from reliable antibodyI load 30 μg of mouse lung tissue lysates per lane ,the primary antibody diluted 1:500, secondary antibody also from Santa cruz use the dilution 1:5000,I used BSA as blocking agent. I got the target band sucessfully.
Date published: 2017-07-01
Rated 5 out of 5 by from Great PEA3 antibody.This antibody is going to give you great results regardless of if you are using mouse, rat, or human. It has nearly 50 citations to support it.
Date published: 2017-01-06
Rated 5 out of 5 by from Strong western dataVery strong western blot results on both mouse and human tissues
Date published: 2017-01-05
Rated 4 out of 5 by from WB: Worked well in mouse NIH3T3 cellsWB: Worked well in mouse NIH3T3 cells. -SCBT Publication Review
Date published: 2015-06-11
Rated 4 out of 5 by from This antibody works well and can be usedThis antibody works well and can be used for IP with human 184B5/HER breast cancer cells. -SCBT Publication Review
Date published: 2015-06-09
Rated 5 out of 5 by from Published IHC data for use in human breast cancersPublished IHC data for use in human breast cancers. -SCBT Publication Review
Date published: 2015-06-02
Rated 5 out of 5 by from Publishable data in WB in human OE33 andPublishable data in WB in human OE33 and OE21 cell line. -SCBT Publication Review
Date published: 2015-05-29
Rated 5 out of 5 by from The X form worked very wellThe X form worked very well, happy with purchase.
Date published: 2015-05-20
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PEA3 抗体 (16) is rated 4.8 out of 5 by 14.
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