Date published: 2025-9-6

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PDIR 抗体 (B-9): sc-390862

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说明书
  • PDIR 抗体 B-9 是小鼠单克隆 IgG2a κ,PDIR抗体, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的PDIR的内部区域内的氨基酸145-164之间
  • PDIR 抗体 (B-9) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 PDIR
  • 抗PDIR抗体(B-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PDIR (B-9): sc-390862免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是PDIR Antibody (B-9) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与PDIR Antibody (B-9) 打包提供(请参阅下面的订购信息)。

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関連項目

PDIR抗体(B-9)是一种IgG2a κ小鼠单克隆PDIR抗体(也称为PDIA5抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的PDIR蛋白。PDIR抗体(B-9)既可作为非偶联的抗PDIR抗体形式提供,也可作为多种偶联形式的抗PDIR抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。氧化还原酶-蛋白质二硫键异构酶(PDI)是一种催化巯基-二硫键交换的同源二聚体,介导新合成蛋白质的折叠并发挥分子伴侣的功能。PDIR(蛋白质二硫键异构酶相关蛋白),也称为PDIA5(蛋白质二硫键异构酶A5),是一种由519个氨基酸组成的蛋白质,催化各种蛋白质中的硫-硫键重排。PDIR定位于内质网(ER)腔中,具有针对α1-抗胰蛋白酶(AAT)的特异性氧化重折叠活性,并有助于在内质网腔中形成二硫键。PDIR在其C端含有一个内质网滞留信号,以及三个硫氧还蛋白(CXXC)基序,这些基序介导PDIR的底物特异性异构酶、伴侣蛋白和氧化还原活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PDIR 抗体 (B-9) 参考文献:

  1. 人类蛋白质-二硫化物异构酶相关蛋白的三个 CXXC 基序对异构酶活性和氧化重折叠的不同贡献。  |  Horibe, T., et al. 2004. J Biol Chem. 279: 4604-11. PMID: 14627699
  2. 用人类蛋白二硫异构酶的结构域 b' 替换人类蛋白二硫异构酶相关蛋白的结构域 b,可显著提高其伴侣活性。  |  Horibe, T., et al. 2004. FEBS Lett. 566: 311-5. PMID: 15147915
  3. 哺乳动物内质网中的氧化蛋白质折叠。  |  Jessop, CE., et al. 2004. Biochem Soc Trans. 32: 655-8. PMID: 15493980
  4. 参与曲格列酮诱导人肝癌细胞系毒性的伴侣蛋白。  |  Maniratanachote, R., et al. 2005. Toxicol Sci. 83: 293-302. PMID: 15525695
  5. 人类蛋白质二硫异构酶家族六种成员的肽硫醇-二硫氧化酶活性的 pH 依赖性。  |  Alanen, HI., et al. 2006. Antioxid Redox Signal. 8: 283-91. PMID: 16677074
  6. 编码新型蛋白质二硫异构酶相关蛋白(PDIR)的 cDNA 分子克隆。  |  Hayano, T. and Kikuchi, M. 1995. FEBS Lett. 372: 210-4. PMID: 7556671

订购信息

产品名称产品编号规格价格数量收藏夹

PDIR 抗体 (B-9)

sc-390862
200 µg/ml
RMB2377.00

PDIR (B-9): m-IgG Fc BP-HRP 套装

sc-530291
200 µg Ab; 10 µg BP
RMB2662.00

PDIR (B-9): m-IgGκ BP-HRP 套装

sc-523714
200 µg Ab, 40 µg BP
RMB2662.00

PDIR 抗体 (B-9) AC

sc-390862 AC
500 µg/ml, 25% agarose
RMB3129.00

PDIR 抗体 (B-9) HRP

sc-390862 HRP
200 µg/ml
RMB2377.00

PDIR 抗体 (B-9) FITC

sc-390862 FITC
200 µg/ml
RMB2482.00

PDIR 抗体 (B-9) PE

sc-390862 PE
200 µg/ml
RMB2580.00

PDIR 抗体 (B-9) Alexa Fluor® 488

sc-390862 AF488
200 µg/ml
RMB2685.00

PDIR 抗体 (B-9) Alexa Fluor® 546

sc-390862 AF546
200 µg/ml
RMB2685.00

PDIR 抗体 (B-9) Alexa Fluor® 594

sc-390862 AF594
200 µg/ml
RMB2685.00

PDIR 抗体 (B-9) Alexa Fluor® 647

sc-390862 AF647
200 µg/ml
RMB2685.00

PDIR 抗体 (B-9) Alexa Fluor® 680

sc-390862 AF680
200 µg/ml
RMB2685.00

PDIR 抗体 (B-9) Alexa Fluor® 790

sc-390862 AF790
200 µg/ml
RMB2685.00

PDIR (B-9) 中和勝肽

sc-390862 P
100 µg/0.5 ml
RMB511.00

what is the sequence that it recognizes, how do I know it is specific to PDIA5/PDIR and does not recognize PDIA1, A2, A3, A4, or A6.

Asked by: Anonymous
Thank you for your question. The epitope for this is 145-164, but the actual sequence is considered proprietary information. Please contact our Technical Service Department and we are happy to address any specific question you may have about the reactivity of this antibody.
Answered by: Technical Service 3
Date published: 2020-11-20

What application is the blocking peptide sc-390862 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Satisfactory Western Blot data of PDIR expressionSatisfactory Western Blot data of PDIR expression in HeLa, Hep G2, HT-1080, MIA PaCa-2 and JEG-3 whole cell lysates. -SCBT QC
Date published: 2015-03-23
Rated 5 out of 5 by from Satisfactory Western Blot data of PDIR expressionSatisfactory Western Blot data of PDIR expression in JAR whole cell lysate. -SCBT QC
Date published: 2014-12-10
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PDIR 抗体 (B-9) is rated 5.0 out of 5 by 2.
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