Date published: 2026-10-24

1-800-457-3801

SCBT Portrait Logo
Seach Input

PCNA 抗体 (PC11): sc-53407

4.0(5)
寫評論提問
说明书
  • PCNA抗体(PC11)是小鼠单克隆IgG1 κ, 在26篇文献中引用,规格为200 µg/ml
  • 针对蛋白A-A-PCNA融合体,从pC2T细胞获得
  • 推荐用于 mouse, rat 和 human 来源的PCNA WB, IP, IF 和 IHC(P)检测
  • 看 PCNA (PC10): sc-56,可查看有标记的 PCNA 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgGκ BP-HRP是PCNA Antibody (PC11) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与PCNA Antibody (PC11) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。
Gene Editing Promo Banner

快捷链接

関連項目

增殖细胞核抗原(PCNA)是一种36 kDa的核蛋白,作为同源三聚体滑动钳,在细胞周期的S期通过增强DNA聚合酶的持续性,对DNA复制和修复至关重要。PCNA主要定位于细胞核,是细胞增殖的重要标志物,有助于科学家研究DNA合成、修复机制以及细胞对DNA损伤的反应。人类PCNA基因位于第20号染色体20p12.3位置。PCNA抗体(PC11)的免疫原为pC2T细胞中的A-A-PCNA融合蛋白,建议使用蛋白质印迹、免疫沉淀、免疫荧光和石蜡包埋切片免疫组织化学等技术检测小鼠、大鼠和人类来源的PCNA。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PCNA 抗体 (PC11) 参考文献:

  1. PCNA相互作用蛋白PARI抑制同源重组。  |  Moldovan, GL., et al. 2012. Mol Cell. 45: 75-86. PMID: 22153967
  2. PCNA 可促进 Exo1 对 DNA 末端的过程性切除。  |  Chen, X., et al. 2013. Nucleic Acids Res. 41: 9325-38. PMID: 23939618
  3. PCNA 泛素化需要 BAF180 的 BAH 结构域。  |  Niimi, A., et al. 2015. Mutat Res. 779: 16-23. PMID: 26117423
  4. ALKBH2 DNA修复酶与PCNA之间的相互作用是直接的,由PCNA的疏水口袋介导,在自然发生的ALKBH2变异中受到干扰。  |  Fu, D., et al. 2015. DNA Repair (Amst). 35: 13-8. PMID: 26408825
  5. 在跨链合成过程中形成PCNA工具带和聚合酶桥。  |  Boehm, EM., et al. 2016. Nucleic Acids Res. 44: 8250-60. PMID: 27325737
  6. PCNA SUMO化可防止PCNA多泛素化介导的、Rad59依赖的、自发性的、染色体内基因转换。  |  Halas, A., et al. 2016. Mutat Res. 791-792: 10-18. PMID: 27505077
  7. PCNA依赖的细胞活性可容忍PCNA客户交互中的剧烈扰动。  |  Wilson, RH., et al. 2017. DNA Repair (Amst). 50: 22-35. PMID: 28073635
  8. PCNA K165修饰在秀丽隐杆线虫配子发生和胚胎发生过程中的重要作用。  |  Shao, Z., et al. 2019. DNA Repair (Amst). 82: 102688. PMID: 31450086
  9. 细胞骨架的完整性会影响 DNA 损伤时 XRCC1 和 PCNA 的动态。  |  Hurst, V., et al. 2021. Mol Biol Cell. 32: br6. PMID: 34379448
  10. PCNA稳定性对突变形成的影响。  |  Arbel-Groissman, M., et al. 2024. Int J Mol Sci. 25: PMID: 39201337

订购信息

产品名称产品编号规格价格数量收藏夹

PCNA 抗体 (PC11)

sc-53407
200 µg/ml
CNY2422.00

PCNA (PC11): m-IgGκ BP-HRP 套装

sc-520977
200 µg Ab, 40 µg BP
CNY2715.00

can i get a free sample of this antibody ? i intend to use pcna for my research

Asked by: atis
Thank you for contacting Santa Cruz Biotechnology. To receive information on our antibody sample program please email [email protected].
Answered by: BlakeJ
Date published: 2025-03-07

I am using PCNA (PC11): sc-53407 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2026, m_10, d_23, h_6CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_53407, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 100ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by MS from Strong WB band in human 293T whole cell lysatesStrong WB band in human 293T whole cell lysates. -SCBT QC
Date published: 2015-05-02
Rated 4 out of 5 by MC from Good specificity in WB and IHC in human MuscularisGood specificity in WB and IHC in human Muscularis mucosa samples. -SCBT Publication Review
Date published: 2015-03-27
Rated 4 out of 5 by NZ from Worked well in WB in AWorked well in WB in A-431, MOLT-4 whole cell lysates and Hel G2, HeLa, CCRF-CEM nuclear extracts. -SCBT QC
Date published: 2015-03-07
Rated 4 out of 5 by TO from Good staining in IHC in rat mammary tumorsGood staining in IHC in rat mammary tumors, ovary, kidney, uterus, lung and liver tissues. -SCBT Publication Review
Date published: 2015-01-09
Rated 4 out of 5 by LM from Strong nuclear localization in IF in methanolStrong nuclear localization in IF in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-07
  • y_2026, m_10, d_23, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_zh_CN, sid_53407, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
PCNA 抗体 (PC11) is rated 4.0 out of 5 by 5.
  • y_2026, m_10, d_23, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_zh_CN, sid_53407, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 150ms
  • REVIEWS, PRODUCT