Date published: 2025-9-6
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PCNA Antibody (PC10) - Western Blotting - Image 399258
PCNA 抗体 (PC10): sc-56. 荧光西部印迹分析 PCNA 在 HCT-116 (A), Raji (B), HeLa (C), MOLT-4 (D), NIH/3T3 (E) 和 KNRK (F) 全细胞裂解液中的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgG2a BP-CFL 647: sc-542738.
PCNA 抗体 (PC10): sc-56. 荧光西部印迹分析 PCNA 在 HCT-116 (A), Raji (B), HeLa (C), MOLT-4 (D), NIH/3T3 (E) 和 KNRK (F) 全细胞裂解液中的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgG2a BP-CFL 647: sc-542738.

PCNA 抗体 (PC10): sc-56

4.9(114)
寫評論提問

说明书
  • PCNA (Clone PC10) is a mouse monoclonal IgG2a κ antibody, currently cited in 2,526 publications, and provided at 200 µg/ml
  • The Calculated Molecular Weight of PCNA is ~29 kDa, however observed weight of PCNA is ~36kDa.
  • Detects PCNA protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM
  • PCNA (proliferating cell nuclear antigen) is an auxiliary protein of DNA polymerase delta that assists in DNA replication and DNA repair.
  • Antibodies for PCNA are antinuclear antibodies (ANAs) typically used to study cell proliferation, DNA replication, and cancer.
  • 抗PCNA抗体（PC10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PCNA (PC10): sc-56的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

PCNA抗体（PC10）是一种IgG2a κ小鼠单克隆PCNA抗体（也称为ATLD2抗体），可通过WB、IP、IF、IHC（P）和FCM检测m、r、h、昆虫、S. pombe来源的PCNA蛋白。PCNA抗体（PC10）可以作为非偶联的抗PCNA抗体形式，也可以作为抗PCNA抗体的多种偶联形式，包括琼脂、HRP、PE、FITC和多种Alexa Fluor®偶联物。PCNA（增殖细胞核抗原）蛋白在细胞周期的早期G1和S期合成，参与细胞周期进程、DNA复制和DNA修复。在早期S期，PCNA蛋白表现为颗粒分布，在核仁中不存在；但是，在晚期S期，它重新定位到核仁中。因此，在染色应用中，PCNA抗体表现为核染色。PCNA存在两种基本形式：一种参与持续DNA复制的形式，其特异性定位于细胞核，另一种不参与持续合成的可溶性形式。有趣的是，后者在有机溶剂的存在下降解，使其在使用有机固定剂的组织中不可检测。因此，只有合成形式可以通过PCNA抗体使用此方法检测到。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PCNA 抗体 (PC10) 参考文献:

  1. E2F 调节的转录对果蝇 PCNA 基因功能的贡献  |  Thacker, SA., et al. 2003. Curr Biol. 13: 53-8. PMID: 12526745
  2. 果蝇错配修复基因和 PCNA 基因同源突变体和杂合突变体微卫星位点上的种系突变。  |  Baida, A., et al. 2003. DNA Repair (Amst). 2: 827-33. PMID: 12826283
  3. 人类增殖细胞核抗原可调节转录辅激活子 p300 的活性并促进转录抑制。  |  Hong, R. and Chakravarti, D. 2003. J Biol Chem. 278: 44505-13. PMID: 12937166
  4. 犰狳/穿山甲调节 PCNA 和 DREF 启动子的活性。  |  Kwon, E., et al. 2004. Biochim Biophys Acta. 1679: 256-62. PMID: 15358517
  5. 将 GFP-PCNA 作为胚胎第一个和随后细胞周期中的 S 期标记。  |  Kisielewska, J., et al. 2005. Biol Cell. 97: 221-9. PMID: 15584900
  6. 果蝇 PCNA 突变体的种系基因组不稳定性：DNA 指纹和微卫星分析  |  López, A., et al. 2005. Mutat Res. 570: 253-65. PMID: 15708584
  7. 评估原发性胃肠道淋巴瘤中的增殖细胞核抗原（PCNA）免疫染色及其与组织学分级, S+G2+M期比例（流式细胞分析）和预后的关系。  |  Woods, AL., et al. 1991. Histopathology. 19: 21-7. PMID: 1680784
  8. 增殖细胞核抗原（PCNA）的单克隆抗体分析。结构保持和核极形式的检测。  |  Waseem, NH. and Lane, DP. 1990. J Cell Sci. 96 (Pt 1): 121-9. PMID: 1695635
  9. 古细菌PCNA蛋白质。  |  Pan, M., et al. 2011. Biochem Soc Trans. 39: 20-4. PMID: 21265741
  10. 细胞周期中存在两种细胞周期蛋白/增殖细胞核抗原：与 DNA 复制位点有关。  |  Bravo, R. and Macdonald-Bravo, H. 1987. J Cell Biol. 105: 1549-54. PMID: 2889739
  11. 鉴别人类DNA聚合酶δ中与PCNA相互作用的蛋白质基序。  |  Khandagale, P., et al. 2020. Biosci Rep. 40: PMID: 32314787
  12. PCNA作为蛋白质纳米标尺用于亚10纳米荧光成像。  |  Helmerich, DA., et al. 2024. Adv Mater. 36: e2310104. PMID: 38009560

订购信息

产品名称产品编号规格价格数量收藏夹

PCNA 抗体 (PC10)

sc-56
200 µg/ml
RMB2377.00

PCNA (PC10): m-IgG Fc BP-HRP 套装

sc-528093
200 µg Ab; 10 µg BP
RMB2662.00

PCNA (PC10): m-IgGκ BP-HRP 套装

sc-520391
200 µg Ab, 40 µg BP
RMB2662.00

PCNA (PC10): m-IgG2a BP-HRP 套装

sc-547021
200 µg Ab; 10 µg BP
RMB2662.00

PCNA 抗体 (PC10) AC

sc-56 AC
500 µg/ml, 25% agarose
RMB3129.00

PCNA 抗体 (PC10) HRP

sc-56 HRP
200 µg/ml
RMB2377.00

PCNA 抗体 (PC10) FITC

sc-56 FITC
200 µg/ml
RMB2482.00

PCNA 抗体 (PC10) PE

sc-56 PE
200 µg/ml
RMB2580.00

PCNA 抗体 (PC10) Alexa Fluor® 488

sc-56 AF488
200 µg/ml
RMB2685.00

PCNA 抗体 (PC10) Alexa Fluor® 546

sc-56 AF546
200 µg/ml
RMB2685.00

PCNA 抗体 (PC10) Alexa Fluor® 594

sc-56 AF594
200 µg/ml
RMB2685.00

PCNA 抗体 (PC10) Alexa Fluor® 647

sc-56 AF647
200 µg/ml
RMB2685.00

PCNA 抗体 (PC10) Alexa Fluor® 680

sc-56 AF680
200 µg/ml
RMB2685.00

PCNA 抗体 (PC10) Alexa Fluor® 790

sc-56 AF790
200 µg/ml
RMB2685.00

PCNA 抗体 (PC10) B

sc-56 B
200 µg/ml
RMB2407.00

PCNA 抗体 (PC10) TRITC

sc-56 TRITC
200 µg/ml
RMB2407.00

PCNA 抗体 (PC10) Alexa Fluor® 405

sc-56 AF405
200 µg/ml
RMB2685.00

Is it necessary to perform an antigen unmasking when using the anti-PCNA (PC10) mAb antibody? If yes, what is the protocol? Thanks  

Asked by: JPS44
Thank you for your question. You can find our recommended protocol with instructions on how to perform antigen unmasking under this link https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Support Europe
Date published: 2024-11-22

What blocking procedure to you recommend for using this mouse monoclonal antibody on mouse tissue?

Asked by: Bscience
Thank you for your question. Incubate specimens for 30 minutes with 10% normal blocking serum in PBS .https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2024-03-28

does antibody m-IgGκ BP-HRP:(sc-516102) compatible to using as secondary antibody against Anti-PCNA Antibody (PC10): sc-56 for western blot

Asked by: Anonymous
Thank you for your question. Anti-PCNA Antibody (PC10) is a mouse monoclonal IgG2a κ PCNA antibody, so it is compatible with m-IgGκ BP-HRP:(sc-516102), which recognizes kappa light chain mouse IgG, IgA and IgM antibodies.
Answered by: Tech Support Europe
Date published: 2023-04-15

Recently we observed that the PCNA sc-56 antibody in the tube is precipitated. It was stored at 4 degrees as recommended. Can the antibody be used for experimental purpose? If yes how do we use it? any recommendation from company's side?

Asked by: Anonymous
Thank you for your question. Please contact technical service directly so they can look into this matter.
Answered by: Tech Support Europe
Date published: 2021-01-18

Which antigen retrieval buffer should be used with PCNA sc-56, citrate or Tris? Can you please include this information in the product datasheet so that it becomes convenient for the user to begin with.

Asked by: Sobia
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this PCNA antibody. The detailed method can be found in our recommended protocol online.
Answered by: Technical Support
Date published: 2025-01-19

Can I use this mouse monoclonal antibody for immunohistochemical staining of swine(pig) tissue?

Asked by: xgtian
Thank you for your question. We have not tested this antibody on swine or pig tissue, however, we are aware of a few publications citing use of this antibody in pig samples. For example, PMID: 26389662.
Answered by: Technical Support
Date published: 2025-02-26

We are new to IHC. Thinking of using this antibody, PCNA Antibody: sc-56on our chicken samples. Wondering if there are any preferences on the conjugate for the secondary antibody, such as HRP, biotin etc. We don't need fluorescence

Asked by: SDas
Thank you for your question. This antibody has been previously cited for detection of PCNA in chicken samples. For IHC, you could use the unconjugated antibody, with an appropriate secondary antibody for detection, or the Biotin conjugated primary with an ABC Staining System, or the HRP conjugate for direct immunoperoxidase staining. Using the directly conjugated primary antibody can save you time and money on reagents. We do not have a preference for this particular antibody, and think you would be successful using either version of the antibody, depending on your preferences in your lab.
Answered by: Technical Support
Date published: 2023-04-15

PCNA Antibody (PC10) how much of 200ug/ml of this antibody I am going to get in one order?

Asked by: Anukana
Thank you for your question. This is provided as 200 ug in 1 mL.
Answered by: Technical Support
Date published: 2025-01-20
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Rated 5 out of 5 by from SC-56 works for rabbitsSC-56 works for rabbits. IHC(P): sections were redyhrated and incubated in sodium citrate buffer (pH6.0) at 85 degree for 30 min for antigen retrieval. Antibody was diluted in 2% goat serum and 1% BSA (1:500).
Date published: 2024-05-15
Rated 5 out of 5 by from Very good antibodyThe antibody works very well in western blot using 30ug of protein
Date published: 2024-04-05
Rated 5 out of 5 by from very good despite low dilution needed1:100 dilution Antigen retrieval: Citrate buffer (pH 6.0) in a microwave
Date published: 2024-02-12
Rated 5 out of 5 by from IHC效果很好strong and specifical signals in IHC-P experiments，staining mouse tissues.
Date published: 2022-11-20
Rated 5 out of 5 by from Works great for IF with super-resolution imagingI got the Alexa-488 conjugated antibody and have used to for IF on U-2 OS cells. There was nuclear specific PCNA labelling. I imaged the samples with dSTORM and SIM methods and the immunolabeling looks great.
Date published: 2021-07-29
Rated 5 out of 5 by from Works very well!We blotted several times with this antibody and it works perfectly!
Date published: 2021-06-16
Rated 5 out of 5 by from PCNA Ab for WB and IFExcellent antibody for WB and IF (fixed with paraformaldehyde) dilution 1:2000 Good housekeeping protein for human colon cancer cell lines
Date published: 2021-03-23
Rated 5 out of 5 by from Excellent antibodyExcellent bands with high dilution and no background
Date published: 2021-03-17
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PCNA 抗体 (PC10) is rated 4.9 out of 5 by 114.
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