Date published: 2025-9-8

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Pax-6 抗体 (AD2.35): sc-53108

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说明书
  • Pax-6抗体(AD2.35)是小鼠单克隆IgG2a κ, 在24篇文献中引用,规格为200 µg/ml
  • 免疫human来源Pax-6位于N-terminus 的1-206氨基酸
  • 抗-Pax-6 抗体 (AD2.35) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat, human 和avian 来源的 Pax-6
  • 抗Pax-6抗体(AD2.35)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Pax-6 (AD2.35): sc-53108免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 Pax-6 抗体 (AD2.35) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Pax-6 抗体 (AD2.35) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Pax-6抗体(AD2.35)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学等方法检测小鼠、大鼠、人类和鸟类中的Pax-6。Anti-Pax-6抗体(AD2.35)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Pax-6在眼睛发育中起着至关重要的作用,它是一种转录调节因子,通过与特定DNA序列结合来控制眼部结构形成所必需的基因的表达。 Pax-6的突变与多种先天性眼部疾病有关,包括无虹膜症,其特征是缺乏虹膜,可导致严重的视力障碍。Pax-6的配对结构域由两个不同的DNA结合子结构域组成,能够与目标基因精确相互作用,从而影响正常眼部发育的途径。了解Pax-6的功能和调节对于阐明眼部疾病的遗传基础和开发潜在的治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Pax-6 抗体 (AD2.35) 参考文献:

  1. 功能性PAX-6基因相关多态区域:与偏执型精神分裂症的可能关联。  |  Stöber, G., et al. 1999. Biol Psychiatry. 45: 1585-91. PMID: 10376119
  2. 胰腺β细胞特异性转录因子Pax-4通过Pax-6抑制胰高血糖素基因的表达。  |  Ritz-Laser, B., et al. 2002. Diabetologia. 45: 97-107. PMID: 11845228
  3. 体内和体外刺胞动物Pax-C和Pax-B蛋白的DNA结合特性:与Pax-6和Pax-2/5/8类没有简单关系。  |  Plaza, S., et al. 2003. J Exp Zool B Mol Dev Evol. 299: 26-35. PMID: 14508814
  4. Pax-6和Pdx-1对胰岛特异性葡萄糖-6-磷酸酶催化亚基相关蛋白基因转录的差异调节。  |  Martin, CC., et al. 2004. J Biol Chem. 279: 34277-89. PMID: 15180990
  5. 在失明后恢复期间,Pax-6 在孵化后小鸡视网膜中的表达。  |  Bhat, SP., et al. 2004. Dev Neurosci. 26: 328-35. PMID: 15855761
  6. 蛋白磷酸酶-1能调节控制大脑和眼睛发育的转录因子Pax-6的功能。  |  Yan, Q., et al. 2007. J Biol Chem. 282: 13954-65. PMID: 17374606
  7. PAX-6基因启动子相关多态性重复与酒精依赖性的关联分析。  |  Samochowiec, J., et al. 1999. Addict Biol. 4: 323-8. PMID: 20575799
  8. 苏莫伊化激活了Pax-6的转录活性,而Pax-6是眼睛和大脑发育的重要转录因子。  |  Yan, Q., et al. 2010. Proc Natl Acad Sci U S A. 107: 21034-9. PMID: 21084637
  9. 髓母细胞瘤中PAX-6和EN蛋白的差异表达。  |  Vincent, S., et al. 1996. Int J Oncol. 8: 901-10. PMID: 21544444
  10. Pax-6对于晶状体中特异性表达的ζ-晶状体蛋白至关重要。  |  Richardson, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 4676-80. PMID: 7753863

订购信息

产品名称产品编号规格价格数量收藏夹

Pax-6 抗体 (AD2.35)

sc-53108
200 µg/ml
RMB2377.00

Pax-6 (AD2.35): m-IgG Fc BP-HRP 套装

sc-525577
200 µg Ab; 10 µg BP
RMB2662.00

Pax-6 (AD2.35): m-IgG2a BP-HRP 套装

sc-546323
200 µg Ab; 10 µg BP
RMB2662.00

Pax-6 抗体 (AD2.35) AC

sc-53108 AC
500 µg/ml, 25% agarose
RMB3129.00

Pax-6 抗体 (AD2.35) HRP

sc-53108 HRP
200 µg/ml
RMB2377.00

Pax-6 抗体 (AD2.35) FITC

sc-53108 FITC
200 µg/ml
RMB2482.00

Pax-6 抗体 (AD2.35) PE

sc-53108 PE
200 µg/ml
RMB2580.00

Pax-6 抗体 (AD2.35) Alexa Fluor® 488

sc-53108 AF488
200 µg/ml
RMB2685.00

Pax-6 抗体 (AD2.35) Alexa Fluor® 546

sc-53108 AF546
200 µg/ml
RMB2685.00

Pax-6 抗体 (AD2.35) Alexa Fluor® 594

sc-53108 AF594
200 µg/ml
RMB2685.00

Pax-6 抗体 (AD2.35) Alexa Fluor® 647

sc-53108 AF647
200 µg/ml
RMB2685.00

Pax-6 抗体 (AD2.35) Alexa Fluor® 680

sc-53108 AF680
200 µg/ml
RMB2685.00

Pax-6 抗体 (AD2.35) Alexa Fluor® 790

sc-53108 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-02-07
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Rated 4 out of 5 by from good for IF on cryosectionsThis antibody worked good for immunofluorescence at 2.0 μg/ml on cryosections of PLP-fixed mouse embryonic telencephalons. No heat-induced epitope retrieval was required.
Date published: 2023-02-21
Rated 4 out of 5 by from good signal in IFgood staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 5 out of 5 by from very good workingThis monoclonal antibody works for the detection of mouse Pax-6 by WB in extracts from mouse kidney. Dilution 1:800,shows strong bands.
Date published: 2017-08-26
Rated 5 out of 5 by from Western blotPax-6 antibody have powerful band detected in a large number of cell lines, good specificity, the analysis results of Western blot were very strong and clear.
Date published: 2017-05-24
Rated 5 out of 5 by from Popular choice for WB and IHC!Pax-6 Antibody (AD2.35) is a popular choice for WB and IHC applications. It has been featured in 8 journal articles, most of them in the last two years!
Date published: 2017-01-21
Rated 4 out of 5 by from Various publishable data in WB using mouseVarious publishable data in WB using mouse cerebellum, calvaria and long bone tissue at embryonic. -SCBT Publication Review
Date published: 2015-04-23
Rated 4 out of 5 by from Publishable data in IHC(P) using rat brainPublishable data in IHC(P) using rat brain. -SCBT Publication Review
Date published: 2015-04-07
Rated 4 out of 5 by from Publishable data in IF using mouse cerebellumPublishable data in IF using mouse cerebellum. -SCBT Publication Review
Date published: 2015-03-25
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Pax-6 抗体 (AD2.35) is rated 4.4 out of 5 by 11.
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