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patched/PTCH1 Antibody (188G11) - Western Blotting - Image 11289
patched 抗体 (188G11): sc-23929. 施耐德果蝇 2 号系全细胞裂解液的 Western 印迹分析.
patched/PTCH1 Antibody (188G11) - Western Blotting - Image 11289
patched 抗体 (188G11): sc-23929. 施耐德果蝇 2 号系全细胞裂解液的 Western 印迹分析.
patched 抗体 (188G11): sc-23929. 施耐德果蝇 2 号系全细胞裂解液的 Western 印迹分析.

patched/PTCH1 抗体 (188G11): sc-23929

3.3(3)
寫評論提問

说明书
  • patched/PTCH1抗体(188G11)是小鼠单克隆IgG1 κ, 在2篇文献中引用，规格为200 µg/ml
  • 免疫drosophila物种的patched protein的氨基酸1194-1286
  • patched/PTCH1 抗体 (188G11) 推荐用于WB, IP 和 IF，检测d 来源的 patched
  • 抗patched/PTCH1抗体（188G11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是patched/PTCH1 Antibody (188G11) for WB applications. 的首选辅助检测试剂，这些试剂现与patched/PTCH1 Antibody (188G11) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

此外 Patched 抗体包括 patched 2

补丁抗体（188G11）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测果蝇源性补丁蛋白。抗补丁抗体（188G11）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。补丁蛋白（又称PTCH1）在刺猬信号传导通路中起着至关重要的作用，该通路在发育过程中调节细胞生长、分化和组织模式。补丁（188G11）抗体主要识别细胞膜中的PTCH1，PTCH1在细胞膜中作为刺猬配体的受体，影响下游信号传导通路。PTCH1在细胞表面的正确定位对于调节细胞极性并确保细胞接收正确的发育信号至关重要。PTCH1功能的紊乱会导致发育异常，并可能与多种癌症有关，这凸显了PTCH1在发育生物学和癌症研究中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

patched/PTCH1 抗体 (188G11) 参考文献:

  1. 黑腹果蝇的基因组序列。  |  Adams, MD., et al. 2000. Science. 287: 2185-95. PMID: 10731132
  2. 果蝇的 patched 基因编码一种节段模式化所需的假定膜蛋白。  |  Hooper, JE. and Scott, MP. 1989. Cell. 59: 751-65. PMID: 2582494
  3. 果蝇节段极性基因 patched 编码的一种具有多个可能跨膜结构域的蛋白质。  |  Nakano, Y., et al. 1989. Nature. 341: 508-13. PMID: 2797178
  4. 影响果蝇体节数目和极性的突变  |  Nüsslein-Volhard, C. and Wieschaus, E. 1980. Nature. 287: 795-801. PMID: 6776413
  5. 基底细胞痣综合征候选基因 patched 的人类同源物。  |  Johnson, RL., et al. 1996. Science. 272: 1668-71. PMID: 8658145

订购信息

产品名称产品编号规格价格数量收藏夹

patched/PTCH1 抗体 (188G11)

sc-23929
200 µg/ml
RMB2377.00

patched/PTCH1 (188G11): m-IgG Fc BP-HRP 套装

sc-526637
200 µg Ab; 10 µg BP
RMB2662.00

patched/PTCH1 (188G11): m-IgG1 BP-HRP 套装

sc-532010
200 µg Ab; 20 µg BP
RMB2662.00

patched/PTCH1 抗体 (188G11) AC

sc-23929 AC
500 µg/ml, 25% agarose
RMB3129.00

patched/PTCH1 抗体 (188G11) HRP

sc-23929 HRP
200 µg/ml
RMB2377.00

patched/PTCH1 抗体 (188G11) FITC

sc-23929 FITC
200 µg/ml
RMB2482.00

patched/PTCH1 抗体 (188G11) PE

sc-23929 PE
200 µg/ml
RMB2580.00

patched/PTCH1 抗体 (188G11) Alexa Fluor® 488

sc-23929 AF488
200 µg/ml
RMB2685.00

patched/PTCH1 抗体 (188G11) Alexa Fluor® 546

sc-23929 AF546
200 µg/ml
RMB2685.00

patched/PTCH1 抗体 (188G11) Alexa Fluor® 594

sc-23929 AF594
200 µg/ml
RMB2685.00

patched/PTCH1 抗体 (188G11) Alexa Fluor® 647

sc-23929 AF647
200 µg/ml
RMB2685.00

patched/PTCH1 抗体 (188G11) Alexa Fluor® 680

sc-23929 AF680
200 µg/ml
RMB2685.00

patched/PTCH1 抗体 (188G11) Alexa Fluor® 790

sc-23929 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for patched (188G11): sc-23929 monoclonal antibody?

Asked by: jerojero
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-23929.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2020-07-27
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Rated 5 out of 5 by from Good for Western BlotAntibody works for Western blot with TPC-1 and BCPAP cell lysates in published research -SCBT QC
Date published: 2023-09-14
Rated 1 out of 5 by from western blothigh concentration antibody and high concentration protein can see weak band.
Date published: 2017-08-17
Rated 4 out of 5 by from RobustRobust, overblown band in western blot analysis of Schneider's Drosophila line 2 whole cell lysate.
Date published: 2013-08-10
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patched/PTCH1 抗体 (188G11) is rated 3.3 out of 5 by 3.
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