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PARP1 Antibody (B-10) - Western Blotting - Image 17028
PARP-1 抗体 (B-10): sc-74470. Jurkat (A), WEHI-231 (B) 和 KNRK (C) 全细胞裂解液中 PARP-1 表达的 Western 印迹分析.
PARP-1 抗体 (B-10): sc-74470. Jurkat (A), WEHI-231 (B) 和 KNRK (C) 全细胞裂解液中 PARP-1 表达的 Western 印迹分析.

PARP1 抗体 (B-10): sc-74470

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寫評論提問

说明书
  • PARP1抗体(B-10)是小鼠单克隆IgG1 κ, 在72篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-300，在PARP-1的human起源的N-terminus处进行映射
  • 抗-PARP1 抗体 (B-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PARP-1
  • 抗PARP1抗体（B-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-74470 X, 200 µg/0.1 ml)
  • 关于如何获取PARP1 (B-10): sc-74470的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 PARP1 抗体 (B-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PARP1 抗体 (B-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加PARP抗体，包括pADPr, PARP-1, PARP-10, PARP-14, PARP-2, PARP-3和PARP-4

PARP-1抗体（B-10）是一种IgG1 κ小鼠单克隆PARP-1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC-P）和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类的PARP-1。PARP-1抗体（B-10）既可以非偶联形式的抗PARP-1抗体提供，也可以多种偶联形式的抗PARP-1抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。多聚（ADP-核糖）聚合酶-1（PARP-1），也称为PARP，是一种核DNA结合锌指蛋白，可影响DNA修复、DNA复制、染色质结构调制和细胞凋亡。作为对遗传毒性应激的响应，PARP-1催化ADP-核糖单位从NAD（+）转移到包括染色质在内的许多受体分子上。PARP-1识别DNA链中断，并能与RNA形成复合物并负调控转录。放线菌素D和依托泊苷依赖性诱导的半胱天冬酶介导PARP-1裂解成p89片段，该片段进入细胞质。凋亡诱导因子（AIF）从线粒体到细胞核的转位依赖于PARP-1，并且是PARP-1依赖性细胞死亡所必需的。PARP-1的缺乏导致由于染色体融合和异倍体频率的增加而引起的染色体不稳定性，这表明多聚（ADP-核糖基）化有助于有效维持基因组完整性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

PARP1 抗体 (B-10)

sc-74470
200 µg/ml
RMB2377.00

PARP1 (B-10): m-IgG Fc BP-HRP 套装

sc-527023
200 µg Ab; 10 µg BP
RMB2662.00

PARP1 (B-10): m-IgG1 BP-HRP 套装

sc-532396
200 µg Ab; 20 µg BP
RMB2662.00

PARP1 抗体 (B-10) X

sc-74470 X
200 µg/0.1 ml
RMB2377.00

PARP1 抗体 (B-10) AC

sc-74470 AC
500 µg/ml, 25% agarose
RMB3129.00

PARP1 抗体 (B-10) HRP

sc-74470 HRP
200 µg/ml
RMB2377.00

PARP1 抗体 (B-10) FITC

sc-74470 FITC
200 µg/ml
RMB2482.00

PARP1 抗体 (B-10) PE

sc-74470 PE
200 µg/ml
RMB2580.00

PARP1 抗体 (B-10) Alexa Fluor® 488

sc-74470 AF488
200 µg/ml
RMB2685.00

PARP1 抗体 (B-10) Alexa Fluor® 546

sc-74470 AF546
200 µg/ml
RMB2685.00

PARP1 抗体 (B-10) Alexa Fluor® 594

sc-74470 AF594
200 µg/ml
RMB2685.00

PARP1 抗体 (B-10) Alexa Fluor® 647

sc-74470 AF647
200 µg/ml
RMB2685.00

PARP1 抗体 (B-10) Alexa Fluor® 680

sc-74470 AF680
200 µg/ml
RMB2685.00

PARP1 抗体 (B-10) Alexa Fluor® 790

sc-74470 AF790
200 µg/ml
RMB2685.00

When should the TransCruz reagent sc-74470 X be used instead of the standard form sc-74470?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 3
Date published: 2017-01-17
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Rated 1 out of 5 by from got strong bands between 50 and 75 kDI got a sample of this antibody and tested it with mouse kidney extract for WB. I only got strong bands between 50 to 75 kD.
Date published: 2022-03-10
Rated 5 out of 5 by from 功能很出色我们长期使用PARP-1（B10）这只单克隆抗体，在小鼠巨噬细胞RAW264.7以及HEK293细胞中都很好用。可用于免疫荧光，wb，以及IP。
Date published: 2020-08-18
Rated 5 out of 5 by from SC-74470 good for western blottingSC-74470 is the Catalog number of the product PARP antibody used in this paper. Our level of satisfaction regarding the quality of the above mentioned product on a scale of 1 to 5, with 5 being the best, is 5. We used the product mainly for Western blotting. The comments I can give to other customers is that, the product is good for Western blotting.
Date published: 2020-08-13
Rated 5 out of 5 by from Very clean antibodyWe recently used this antibody to measure PARP-1 cleavage in treated or untreated MCF7 cell lysates. We loaded 10 µg of protein on a 12% polyacrylamide gel and got a strong, clean signal. We are very happy with this antibody and will definitely use it again.
Date published: 2018-02-08
Rated 5 out of 5 by from 功能很出色我一个月前买了这个产品，我为此感到很高兴。我一个月前买了这个产品，我为此感到很高兴。我一个月前买了这个产品，我为此感到很高兴。我一个月前买了这个产品，我为此感到很高兴。我一个月前买了这个产品，我为此感到很高兴。我一个月前买了这个产品，我为此感到很高兴。
Date published: 2017-12-22
Rated 2 out of 5 by from Lots of non-specific bands by western blotting.I tested this antibody by western blotting in wt and PARP1-ko mouse cells. Even though a band with the expected molecular mass of PARP-1 was only detected in wt cells, this antibody also picked up a lots of faster- and slower-migrating bands. Membranes were incubated overnight at 4C with 1:500 dilution of the antibody in 5% milk in TBS-0.1% Tween-20.
Date published: 2017-12-12
Rated 5 out of 5 by from Great antibody!Got a free sample and it worked great for our rat cell line. Got very strong signal. Detects both full lenght and N-terminal cleavage product well. Used in WB at 1:1000.
Date published: 2017-11-15
Rated 1 out of 5 by from The size of PARP1 band is much lower.I bought this antibody a coup of months ago. I want to use it to do IP, but the size was not located in 110 kDa or 89 kDa. it was around 58 kDa. I can't use it to start my IP work and email you the evidence.
Date published: 2017-06-05
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PARP1抗体 (B-10) is rated 4.2 out of 5 by 17.
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