Date published: 2025-9-8

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PARP1 抗体 (42): sc-136208

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说明书
  • PARP1 抗体 42 是小鼠单克隆 IgG1, 在22篇文献中引用,提供 50 µg/0.5 ml
  • 免疫human物种的PARP-1的氨基酸22-219
  • 推荐用于 mouse, rat 和 human 来源的PARP-1 WB, IP 和 IF检测; 也和以下物种反应,包括: canine
  • 看 PARP1 (F-2): sc-8007,可查看有标记的 PARP1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成PARP1 抗体 (42)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

多聚(ADP-核糖)聚合酶-1(PARP-1),也称为PARP,是一种核DNA结合锌指蛋白,可影响DNA修复、DNA复制、染色质结构调制和细胞凋亡。作为对遗传毒性应激的响应,PARP-1催化ADP-核糖单位从NAD(+)转移到包括染色质在内的许多受体分子上。PARP-1识别DNA链中断,并能与RNA形成复合物并负调控转录。放线菌素D和依托泊苷依赖性诱导的半胱天冬酶介导PARP-1裂解成p89片段,该片段进入细胞质。凋亡诱导因子(AIF)从线粒体到细胞核的转位依赖于PARP-1,并且是PARP-1依赖性细胞死亡所必需的。PARP-1的缺乏导致由于染色体融合和异倍体频率的增加而引起的染色体不稳定性,这表明多聚(ADP-核糖基)化有助于有效维持基因组完整性。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

PARP1 抗体 (42)

sc-136208
50 µg/0.5 ml
RMB2377.00

有PARP1流式抗体吗?这个抗体(sc-136208)有荧光标记吗?

Asked by: yongshu
感谢您的提问。PARP-1目前没有推荐用于流式实验的抗体。sc-136208本身没有荧光标记。如果 您需要定制荧光标记的sc-136208抗体,请通过电话(+86) 021-60936351、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. This antibody has not been tested for Flow Cytometry. sc-136208 itself is not fluorescein conjugated. If you wish to special order a fluorescein conjugated form or want to have more information, please contact our Asian Technical Service team at (+86) 021-60936351 or [email protected] or by live chat on our website.
Answered by: Tech Service
Date published: 2017-03-27

What is the recommended fixation method for immunofluorescence staining with PARP-1 (42): sc-136208 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from 功能很出色!效果很不错!功能很出色!出了想要的结果,太感谢了!赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞!
Date published: 2018-06-16
Rated 5 out of 5 by from goodvery well,very clean and strong bangs in WB in human cell
Date published: 2017-09-11
Rated 5 out of 5 by from AwesomeWe used to check purity of cytosolic/ nuclear fraction. Prose: easy to detect, strong, single band. cons: none.
Date published: 2017-06-28
Rated 5 out of 5 by from the parp antibody is very goodthe product is wonderful,I use the antidody with the 1:200 dilution,the WB band is clear.
Date published: 2017-03-29
Rated 5 out of 5 by from perfectI used it for the detection of human protein expression,It is a very good antibody.
Date published: 2016-11-17
Rated 4 out of 5 by from Robust immunofluorescence nuclear stainingRobust immunofluorescence nuclear staining in BC3H1 cells. -SCBT QC
Date published: 2015-03-11
Rated 4 out of 5 by from Worked well for IF with methanolWorked well for IF with methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-03
Rated 4 out of 5 by from WB: Great data obtained using Jurkat wholeWB: Great data obtained using Jurkat whole cell lysates. -SCBT QC
Date published: 2015-01-19
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PARP1 抗体 (42) is rated 4.6 out of 5 by 9.
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