Date published: 2025-9-7

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PARD6B 抗体 (B-10): sc-166405

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说明书
  • PARD6B 抗体 B-10 是小鼠单克隆 IgG2a κ,PARD6B抗体, 在23篇文献中引用,规格为200 µg/ml
  • 针对氨基酸308-371的抗原,在PARD6B的C-terminus处映射,mouse来源
  • PARD6B 抗体 (B-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 PARD6B
  • 抗PARD6B抗体(B-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PARD6B (B-10): sc-166405免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 PARD6B 抗体 (B-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PARD6B 抗体 (B-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

PARD6B抗体(B-10)是一种IgG2a κ小鼠单克隆PARD6B抗体(也称为PARD6抗体),通过WB, IP, IF, IHC(P) 和 ELISA检测小鼠、大鼠和人源的PARD6B蛋白。PARD6B抗体(B-10)既以非偶联的抗PARD6B抗体形式提供,也以多种偶联形式的抗PARD6B抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞不对称性对于多细胞生物的发育至关重要。PARD(分区缺陷)蛋白在不对称细胞分裂和极性生长中发挥重要作用,而Cdc42和Rac则介导细胞生长和极性的建立,并有助于Ras的致癌转化。人类PARD6是一种由345个氨基酸组成的多肽,具有PDZ结构域和类似CRIB(Cdc42/Rac相互作用结合)的基序。PARD6通过此基序与GTP结合的Rac和Cdc42相互作用,并通过N端头对头关联与非典型PKC异构体PKCι/λ和PKCΩ相互作用。这些相互作用允许在体内外形成三元复合物,该复合物与上皮细胞-细胞接触处正常紧密连接的形成有关,也参与秀丽隐杆线虫不对称细胞分裂前母细胞的极化。PARD6通过定位或维持PARD3蛋白在细胞外周的作用来通过PARD3发挥作用。PARD6A,也被称作PAR-6α、PAR6C、TAX40和TIP-40,在胰腺、骨骼肌、脑和心脏中表达,在肾脏和胎盘中弱表达。PAR6B在胰腺以及成人和胎儿肾脏中表达,在胎盘和肺中弱表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PARD6B 抗体 (B-10) 参考文献:

  1. 线虫极性决定子 Par-6 的人类同源物将 Rac 和 Cdc42 与 PKCzeta 信号转导和细胞转化联系起来。  |  Qiu, RG., et al. 2000. Curr Biol. 10: 697-707. PMID: 10873802
  2. 细胞极性蛋白 Par6 将 Par3 和非典型蛋白激酶 C 与 Cdc42 连接起来。  |  Joberty, G., et al. 2000. Nat Cell Biol. 2: 531-9. PMID: 10934474
  3. 哺乳动物 PAR-3-PAR-6 复合物与 Cdc42/Rac1 和 aPKC 信号以及细胞极性有关。  |  Lin, D., et al. 2000. Nat Cell Biol. 2: 540-7. PMID: 10934475
  4. 细胞极性:Cdc42 和 Rac 的新 PARtner。  |  Kim, SK. 2000. Nat Cell Biol. 2: E143-5. PMID: 10934484
  5. 哺乳动物草履虫极性蛋白 PAR-6 的同源物是 Rho GTPase Cdc42 和 Rac1 的结合伙伴。  |  Johansson, A., et al. 2000. J Cell Sci. 113 (Pt 18): 3267-75. PMID: 10954424
  6. 细胞极性:卓越的支架蛋白  |  Brazil, DP. and Hemmings, BA. 2000. Curr Biol. 10: R592-4. PMID: 10985375
  7. 草履虫细胞极性蛋白 PAR6 的人类同源物是连接小 GTP 酶 Rac 和 Cdc42 与非典型蛋白激酶 C 的适配体。  |  Noda, Y., et al. 2001. Genes Cells. 6: 107-19. PMID: 11260256
  8. par-6是一个参与建立早期秀丽隐杆线虫胚胎不对称的基因,它介导了PAR-3的不对称定位。  |  Watts, JL., et al. 1996. Development. 122: 3133-40. PMID: 8898226

订购信息

产品名称产品编号规格价格数量收藏夹

PARD6B 抗体 (B-10)

sc-166405
200 µg/ml
RMB2377.00

PARD6B (B-10): m-IgG Fc BP-HRP 套装

sc-528956
200 µg Ab; 10 µg BP
RMB2662.00

PARD6B (B-10): m-IgGκ BP-HRP 套装

sc-521593
200 µg Ab, 40 µg BP
RMB2662.00

PARD6B (B-10): m-IgG2a BP-HRP 套装

sc-547216
200 µg Ab; 10 µg BP
RMB2662.00

PARD6B 抗体 (B-10) AC

sc-166405 AC
500 µg/ml, 25% agarose
RMB3129.00

PARD6B 抗体 (B-10) HRP

sc-166405 HRP
200 µg/ml
RMB2377.00

PARD6B 抗体 (B-10) FITC

sc-166405 FITC
200 µg/ml
RMB2482.00

PARD6B 抗体 (B-10) PE

sc-166405 PE
200 µg/ml
RMB2580.00

PARD6B 抗体 (B-10) Alexa Fluor® 488

sc-166405 AF488
200 µg/ml
RMB2685.00

PARD6B 抗体 (B-10) Alexa Fluor® 546

sc-166405 AF546
200 µg/ml
RMB2685.00

PARD6B 抗体 (B-10) Alexa Fluor® 594

sc-166405 AF594
200 µg/ml
RMB2685.00

PARD6B 抗体 (B-10) Alexa Fluor® 647

sc-166405 AF647
200 µg/ml
RMB2685.00

PARD6B 抗体 (B-10) Alexa Fluor® 680

sc-166405 AF680
200 µg/ml
RMB2685.00

PARD6B 抗体 (B-10) Alexa Fluor® 790

sc-166405 AF790
200 µg/ml
RMB2685.00

Hi, I dont want to buy a whole aliquot of this antibody before I know it works so do you have a sample size available for this? PARD6B Antibody (B-10): sc-166405

Asked by: CHITKALE
Thank you for your question. Please contact our Technical Service Department to arrange a free 10 µg sample of PARD6B (B-10): sc-166405.
Answered by: Tech Support Europe
Date published: 2021-01-25

I am using PARD6B (B-10): sc-166405 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good WB resultGood WB result for WB analysis of rat brain tissue, the result is very satisfactory.
Date published: 2017-02-12
Rated 5 out of 5 by from Recommended use.Satisfactory Western blot data of RANK expression in non-transfected and mouse RANK transfected 293T whole cell lysates,recommended use.
Date published: 2017-02-08
Rated 5 out of 5 by from Recommended Use.Used PARD6B antibody for WB detection and it worked well. The analysis results of Western blot were very strong and clear.Recommended use
Date published: 2017-02-06
Rated 5 out of 5 by from Very good antibodyThe PARD6B Antibody (B-10): sc-166405 provides excellent data for IHC with formalin fixed, paraffin-embedded tissue sections showing cytoplasmic localized staining.
Date published: 2017-01-17
Rated 5 out of 5 by from Recommend a good partnerAntibodies particularly well in the process of the experiment, we particularly like the antibodies. Will recommend to everyone.
Date published: 2017-01-16
Rated 5 out of 5 by from Strong band in WB in HeLaStrong band in WB in HeLa, PC-3, Caki-1, Jurkat and RAW 264.7 whole cell lysates. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from Good specificity in WB in HeLa and NIH/3T3Good specificity in WB in HeLa and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-01-13
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PARD6B 抗体 (B-10) is rated 5.0 out of 5 by 8.
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