Date published: 2025-9-6

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PARD6A 抗体 (C-3): sc-365323

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说明书
  • PARD6A 抗体 C-3 是小鼠单克隆 IgG1 κ,PARD6A抗体, 在7篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的PARD6A的N-terminus的氨基酸2-27之间
  • PARD6A 抗体 (C-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 PARD6A; 也和以下物种反应,包括: 和 equine, bovine and porcine
  • 抗PARD6A抗体(C-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PARD6A (C-3): sc-365323免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 PARD6A 抗体 (C-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PARD6A 抗体 (C-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

PARD6A 抗体(C-3)是一种 IgG1 κ 小鼠单克隆 PARD6A 抗体(也称为 PAR6 抗体或 PAR6A 抗体),可通过 WB、IP、IF、IHC(P) 和 ELISA 检测小鼠、大鼠和人类来源的 PARD6A 蛋白。PARD6A 抗体(C-3)既可以是非偶联的抗 PARD6A 抗体形式,也可以是多种偶联形式的抗 PARD6A 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。细胞不对称性对于多细胞生物的发展至关重要。PARD(分区缺陷)蛋白在不对称细胞分裂和极性生长中起重要作用,而 Cdc42 和 Rac 则介导细胞生长和极性的建立,并有助于 Ras 的致癌转化。人类 PARD6 是一种由 345 个氨基酸组成的多肽,具有 PDZ 结构域和类似 CRIB(Cdc42/Rac 相互作用结合)的基序。PARD6 通过该基序与 GTP 结合的 Rac 和 Cdc42 相互作用,并通过 N 端头对头关联与非典型 PKC 异构体 PKCι/λ 和 PKCΩ 相互作用。这些相互作用允许在体内外形成三元复合物,该复合物与上皮细胞-细胞接触处正常紧密连接的形成有关,也参与秀丽隐杆线虫不对称细胞分裂前母细胞的极化。PARD6 通过定位或维持 PARD3 蛋白在细胞外周的作用而通过 PARD3 发挥作用。PARD6A,也称为 PAR-6α、PAR6C、TAX40 和 TIP-40,在胰腺、骨骼肌、脑和心脏中表达,在肾脏和胎盘中弱表达。PAR6B 在胰腺以及成人和胎儿肾脏中表达,在胎盘和肺中弱表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PARD6A 抗体 (C-3) 参考文献:

  1. 线虫极性决定子 Par-6 的人类同源物将 Rac 和 Cdc42 与 PKCzeta 信号转导和细胞转化联系起来。  |  Qiu, RG., et al. 2000. Curr Biol. 10: 697-707. PMID: 10873802
  2. 细胞极性蛋白 Par6 将 Par3 和非典型蛋白激酶 C 与 Cdc42 连接起来。  |  Joberty, G., et al. 2000. Nat Cell Biol. 2: 531-9. PMID: 10934474
  3. 哺乳动物 PAR-3-PAR-6 复合物与 Cdc42/Rac1 和 aPKC 信号以及细胞极性有关。  |  Lin, D., et al. 2000. Nat Cell Biol. 2: 540-7. PMID: 10934475
  4. 细胞极性:Cdc42 和 Rac 的新 PARtner。  |  Kim, SK. 2000. Nat Cell Biol. 2: E143-5. PMID: 10934484
  5. 哺乳动物草履虫极性蛋白 PAR-6 的同源物是 Rho GTPase Cdc42 和 Rac1 的结合伙伴。  |  Johansson, A., et al. 2000. J Cell Sci. 113 (Pt 18): 3267-75. PMID: 10954424
  6. 细胞极性:卓越的支架蛋白  |  Brazil, DP. and Hemmings, BA. 2000. Curr Biol. 10: R592-4. PMID: 10985375
  7. 草履虫细胞极性蛋白 PAR6 的人类同源物是连接小 GTP 酶 Rac 和 Cdc42 与非典型蛋白激酶 C 的适配体。  |  Noda, Y., et al. 2001. Genes Cells. 6: 107-19. PMID: 11260256
  8. 人类 Inscuteable 基因的鉴定和特征描述。  |  Katoh, M. and Katoh, M. 2003. Int J Mol Med. 11: 111-6. PMID: 12469229
  9. CREB占位的全基因组筛选确定了RhoA抑制剂Par6C和Rnd3作为BDNF诱导的突触形成的调节因子。  |  Lesiak, A., et al. 2013. PLoS One. 8: e64658. PMID: 23762244
  10. 基于缺氧基因的加权相关网络分析,开发低级别胶质瘤的放射敏感性预测模型。  |  Du, Z., et al. 2022. Front Oncol. 12: 757686. PMID: 35280808
  11. PARD6A通过Serpina3促进肺腺癌细胞的增殖和侵袭。  |  Hu, L., et al. 2024. Cancer Gene Ther. 31: 1696-1707. PMID: 39300216
  12. par-6是一个参与建立早期秀丽隐杆线虫胚胎不对称的基因,它介导了PAR-3的不对称定位。  |  Watts, JL., et al. 1996. Development. 122: 3133-40. PMID: 8898226

订购信息

产品名称产品编号规格价格数量收藏夹

PARD6A 抗体 (C-3)

sc-365323
200 µg/ml
RMB2377.00

PARD6A (C-3): m-IgG Fc BP-HRP 套装

sc-527355
200 µg Ab; 10 µg BP
RMB2662.00

PARD6A (C-3): m-IgG1 BP-HRP 套装

sc-532728
200 µg Ab; 20 µg BP
RMB2662.00

PARD6A 抗体 (C-3) AC

sc-365323 AC
500 µg/ml, 25% agarose
RMB3129.00

PARD6A 抗体 (C-3) HRP

sc-365323 HRP
200 µg/ml
RMB2377.00

PARD6A 抗体 (C-3) FITC

sc-365323 FITC
200 µg/ml
RMB2482.00

PARD6A 抗体 (C-3) PE

sc-365323 PE
200 µg/ml
RMB2580.00

PARD6A 抗体 (C-3) Alexa Fluor® 488

sc-365323 AF488
200 µg/ml
RMB2685.00

PARD6A 抗体 (C-3) Alexa Fluor® 546

sc-365323 AF546
200 µg/ml
RMB2685.00

PARD6A 抗体 (C-3) Alexa Fluor® 594

sc-365323 AF594
200 µg/ml
RMB2685.00

PARD6A 抗体 (C-3) Alexa Fluor® 647

sc-365323 AF647
200 µg/ml
RMB2685.00

PARD6A 抗体 (C-3) Alexa Fluor® 680

sc-365323 AF680
200 µg/ml
RMB2685.00

PARD6A 抗体 (C-3) Alexa Fluor® 790

sc-365323 AF790
200 µg/ml
RMB2685.00

PARD6A (C-3) 中和勝肽

sc-365323 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-365323 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from works wellThe antibody works well for IHC for paraffin section of rat brain. It's shows good quality for detection. Positive staining.
Date published: 2017-08-26
Rated 5 out of 5 by from performs really wellThe antibody performs really well for immunofluorescence in cultured cells.
Date published: 2017-05-28
Rated 5 out of 5 by from clear signalIt was used to detect IL-1 beta in rat brain. Bands were clear and no nonspecific bands.
Date published: 2017-02-12
Rated 5 out of 5 by from The product is good.PARD6A antibody provides clear and specific results. We tried it detect this sample from dilution 1:500.
Date published: 2017-02-09
Rated 5 out of 5 by from The effect is obviousThis product has a strong band and clean background, the effect is very obvious
Date published: 2017-01-04
Rated 5 out of 5 by from strongUsed this antibody in IP and WB at 1:500 and worked well in mouse cells,easy to operate, like Santa antibodies
Date published: 2016-12-17
Rated 5 out of 5 by from Excellent immunofluorescence cytoplasmicExcellent immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from Good specificity in WB in human transfectedGood specificity in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-15
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PARD6A 抗体 (C-3) is rated 5.0 out of 5 by 8.
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