Date published: 2025-9-7
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Parafibromin Antibody (2H1) - Western Blotting - Image 48171
Parafibromin 抗体 (2H1): sc-33638. 未转染 293T: sc-117752 (A), 小鼠 Parafibromin 转染 293T: sc-122375 (B) 和 C32 (C) 全细胞裂解液中 Parafibromin 表达的 Western 印迹分析.
Parafibromin 抗体 (2H1): sc-33638. 未转染 293T: sc-117752 (A), 小鼠 Parafibromin 转染 293T: sc-122375 (B) 和 C32 (C) 全细胞裂解液中 Parafibromin 表达的 Western 印迹分析.

Parafibromin 抗体 (2H1): sc-33638

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说明书
  • Parafibromin 抗体 2H1 是小鼠单克隆 IgG1 κ，Parafibromin抗体, 在61篇文献中引用，规格为200 µg/ml
  • 针对与氨基酸87-100对应的肽，其映射到Parafibromin的，mouse的起源
  • Parafibromin 抗体 (2H1) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Parafibromin
  • 抗Parafibromin抗体（2H1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Parafibromin (2H1): sc-33638的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Parafibromin 抗体 (2H1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Parafibromin 抗体 (2H1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Parafibromin 抗体 (2H1) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和免疫组织化学检测小鼠、大鼠和人类来源的 Parafibromin 蛋白。抗Parafibromin抗体（2H1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。由CDC73基因编码的Parafibromin在调节基因表达方面起着关键作用，是RNA聚合酶II/Paf1复合物的重要组成部分，而RNA聚合酶II/Paf1复合物对于组蛋白修饰和转录调控至关重要。这种复合物对于RNA聚合酶II的正常运作至关重要，因为Parafibromin可促进组蛋白修饰，影响染色质结构和基因可及性。帕拉纤维蛋白是一种诊断甲状旁腺癌的潜在生物标记物，这种癌症的特征是异质性肿瘤，可能使诊断复杂化。人类CDC73基因位于1q25染色体上，是酵母Cdc73的同源基因，与甲状旁腺功能亢进伴颌骨肿瘤综合征（HPT-JT）有关，这凸显了帕拉纤维蛋白在抑制内分泌肿瘤方面的临床意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Parafibromin 抗体 (2H1) 参考文献:

  1. 家族性孤立甲状旁腺功能亢进症很少是由甲状旁腺功能亢进-颌骨肿瘤综合征基因HRPT2的种系突变引起的。  |  Simonds, WF., et al. 2004. J Clin Endocrinol Metab. 89: 96-102. PMID: 14715834
  2. 葡萄牙罗姆人家族中的甲状旁腺功能亢进-颌骨肿瘤综合征是由HRPT2基因的一个创始突变引起的。  |  Cavaco, BM., et al. 2004. J Clin Endocrinol Metab. 89: 1747-52. PMID: 15070940
  3. 副纤维蛋白免疫活性的丧失是甲状旁腺癌的一个显著特征。  |  Tan, MH., et al. 2004. Clin Cancer Res. 10: 6629-37. PMID: 15475453
  4. 甲状旁腺肿瘤的基因表达：分子亚分类和潜在恶性表型的鉴定。  |  Haven, CJ., et al. 2004. Cancer Res. 64: 7405-11. PMID: 15492263
  5. 原发性甲状旁腺功能亢进症中HRPT2基因的遗传分析：家族性和散发性甲状旁腺肿瘤中的种系突变和体细胞突变。  |  Cetani, F., et al. 2004. J Clin Endocrinol Metab. 89: 5583-91. PMID: 15531515
  6. parafibromin肿瘤抑制蛋白是人类Paf1复合体的一部分。  |  Rozenblatt-Rosen, O., et al. 2005. Mol Cell Biol. 25: 612-20. PMID: 15632063
  7. 副纤维蛋白的核极定位由三个核极定位信号介导。  |  Hahn, MA. and Marsh, DJ. 2007. FEBS Lett. 581: 5070-4. PMID: 17923126
  8. 甲状旁腺癌患者副纤维蛋白染色和 CDC73 基因突变的预后作用：基于患者个体数据的系统回顾和荟萃分析。  |  Zhu, R., et al. 2020. Clin Endocrinol (Oxf). 92: 295-302. PMID: 31945198
  9. 副纤维蛋白在肝细胞癌中高度表达，其表达与不良预后相关。  |  Kim, MK. 2022. J Clin Med. 11: PMID: 35407381
  10. 纤维瘤素在甲状旁腺癌中的表达诊断意义。  |  Gao, Y., et al. 2022. Hum Pathol. 127: 28-38. PMID: 35654240
  11. 肿瘤抑制因子纤维瘤素在癌症中的作用。  |  Zheng, HC., et al. 2022. Front Cell Dev Biol. 10: 1006400. PMID: 36211470
  12. 原发性甲状旁腺功能亢进患者中，副纤维蛋白表达与棕色肿瘤和颌骨肿瘤之间的关系：文献回顾系列病例。  |  Popow, M., et al. 2022. Am J Case Rep. 23: e936135. PMID: 36271606

订购信息

产品名称产品编号规格价格数量收藏夹

Parafibromin 抗体 (2H1)

sc-33638
200 µg/ml
RMB2377.00

Parafibromin (2H1): m-IgG Fc BP-HRP 套装

sc-526693
200 µg Ab; 10 µg BP
RMB2662.00

Parafibromin (2H1): m-IgG1 BP-HRP 套装

sc-532066
200 µg Ab; 20 µg BP
RMB2662.00

Parafibromin 抗体 (2H1) AC

sc-33638 AC
500 µg/ml, 25% agarose
RMB3129.00

Parafibromin 抗体 (2H1) HRP

sc-33638 HRP
200 µg/ml
RMB2377.00

Parafibromin 抗体 (2H1) FITC

sc-33638 FITC
200 µg/ml
RMB2482.00

Parafibromin 抗体 (2H1) PE

sc-33638 PE
200 µg/ml
RMB2580.00

Parafibromin 抗体 (2H1) Alexa Fluor® 488

sc-33638 AF488
200 µg/ml
RMB2685.00

Parafibromin 抗体 (2H1) Alexa Fluor® 546

sc-33638 AF546
200 µg/ml
RMB2685.00

Parafibromin 抗体 (2H1) Alexa Fluor® 594

sc-33638 AF594
200 µg/ml
RMB2685.00

Parafibromin 抗体 (2H1) Alexa Fluor® 647

sc-33638 AF647
200 µg/ml
RMB2685.00

Parafibromin 抗体 (2H1) Alexa Fluor® 680

sc-33638 AF680
200 µg/ml
RMB2685.00

Parafibromin 抗体 (2H1) Alexa Fluor® 790

sc-33638 AF790
200 µg/ml
RMB2685.00

Hi , is there the peptide for Parafibromin (2H1); SC-33638 available?

Asked by: Kanyarat
Thank you for your question. Unfortunately we do not have a blocking peptide available for this Parafibromin antibody. If you have any further questions, please reach out to Technical support at [email protected], or via phone at 800-457-3801, ext 2 or via chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-12-04

What is the best secondary to use with this antibody in Western Blot?

Asked by: AbPolly
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 12
Date published: 2017-01-04
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Rated 1 out of 5 by from Does not workI tried 1:500, 1:150 and even 1:50 for my WB. No signal. What a waste of money.
Date published: 2025-05-16
Rated 2 out of 5 by from Weak for WBThis antibody gave me a weak but specific band at 1:200 dilution.
Date published: 2018-05-12
Rated 1 out of 5 by from no antibodyThe tube of parafibromin antibody is empty. Therefore I can't give an appropriate common.
Date published: 2018-02-26
Rated 5 out of 5 by from ParafibrominExcelent antibody for western blot. Detected parafibromin at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti Parafibromin in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse (sc-358914) HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
Date published: 2017-09-25
Rated 5 out of 5 by from OutstandingVery strong western blot and ihc results; best clone
Date published: 2016-12-31
Rated 5 out of 5 by from Works wellSpecific staining seen in IHC and clean bands in Western Blot at 1:50 and 1:100 respectively.
Date published: 2016-12-30
Rated 5 out of 5 by from Published WB data on HeLaPublished WB data on HeLa, HEK293TN and IMEC lines
Date published: 2016-06-24
Rated 5 out of 5 by from 2H1 used at 1:40 dilution2H1 used at 1:40 dilution - incubated for 15 min for immunohistochemical staining of human gastric carcinoma sections. -SCBT Publication Review
Date published: 2015-07-07
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Parafibromin 抗体 (2H1) is rated 4.1 out of 5 by 12.
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