Date published: 2025-9-7
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PAR-2 Antibody (SAM11) - Western Blotting - Image 398264
PAR-2 抗体 (SAM11): sc-13504. Hep G2 (A), WiDR (B), NIH/3T3 (C), F9 (D), c4 (E) 和 3611-RF (F) 全细胞裂解液中 PAR-2 表达的 Western 印迹分析. 所用检测试剂: m-IgG Fc BP-HRP: sc-525409.
PAR-2 抗体 (SAM11): sc-13504. Hep G2 (A), WiDR (B), NIH/3T3 (C), F9 (D), c4 (E) 和 3611-RF (F) 全细胞裂解液中 PAR-2 表达的 Western 印迹分析. 所用检测试剂: m-IgG Fc BP-HRP: sc-525409.

PAR-2 抗体 (SAM11): sc-13504

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说明书
  • PAR-2 抗体 SAM11 是小鼠单克隆 IgG2a κ，PAR-2抗体, 在263篇文献中引用，规格为200 µg/ml
  • 表位定位到PAR-2的37-50氨基酸，该氨基酸来自human
  • PAR-2 抗体 (SAM11) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 PAR-2
  • 抗PAR-2抗体（SAM11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还提供无叠氮化物的中和研究，sc-13504 L，200 µg/0.1 ml
  • 关于如何获取PAR-2 (SAM11): sc-13504的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 PAR-2 抗体 (SAM11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PAR-2 抗体 (SAM11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PAR 抗体 包括 PAR-2, PAR-3, PAR-4和Thrombin R

PAR-2抗体（SAM11）是一种小鼠单克隆IgG2a kappa轻链抗体，用于检测小鼠、大鼠和人类中的蛋白酶激活受体2（PAR-2），也称为F2RL1或PAR2，可用于蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）等应用。PAR-2 单克隆抗体（SAM11）靶向人类 PAR-2 蛋白 37-50 氨基酸内的表位，可在各种实验条件下精确识别和分析 PAR-2。PAR-2 是一种 G 蛋白偶联受体，通过与细胞外蛋白酶（如胰蛋白酶）相互作用，发挥调节炎症反应和疼痛感知的关键作用，胰蛋白酶可裂解并激活受体。PAR-2位于细胞表面，可促进关键的信号传导通路，从而调节细胞对环境刺激的反应，因此其在生理和病理过程中的作用离不开细胞定位。从结构上看，PAR-2具有七个跨膜结构域，这一特征对于其与G蛋白的相互作用以及随后的细胞内信号级联至关重要。了解PAR-2的结构对于阐明其作用机制和开发靶向疗法至关重要。PAR-2单克隆抗体（SAM11）有非结合型和多种结合型，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®变体，为各种研究应用提供了多种选择。PAR-2 单克隆抗体（SAM11）是研究人员研究 PAR-2 介导的细胞功能的分子机制及其在炎症等疾病中的意义的宝贵工具，是推进生物医学研究的关键组成部分。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PAR-2 抗体 (SAM11) 参考文献:

  1. 蛋白酶活化受体 1 和 4 介导凝血酶对人体血小板的激活。  |  Kahn, ML., et al. 1999. J Clin Invest. 103: 879-87. PMID: 10079109
  2. PAR-1和PAR-2在发炎牙细胞中的表达增强。  |  Alvarez, MMP., et al. 2017. J Dent Res. 96: 1518-1525. PMID: 28759300
  3. 表达荧光PAR2的小鼠揭示了内吞作用介导结肠炎症和疼痛。  |  Latorre, R., et al. 2022. Proc Natl Acad Sci U S A. 119: PMID: 35110404
  4. Matriptase通过PAR-2/PI3K/Akt/MMP9信号轴驱动卵巢癌球体的扩散。  |  Pawar, NR., et al. 2023. J Cell Biol. 222: PMID: 37737895
  5. PAR-2参与激活蛋白C诱导皮肤伤口愈合中细胞特异性基质金属蛋白酶-2的诱导。  |  Julovi, SM., et al. 2023. Int J Mol Sci. 25: PMID: 38203540
  6. 人类角质形成细胞中存在不同于凝血酶受体的蛋白酶活化受体的证据。  |  Santulli, RJ., et al. 1995. Proc Natl Acad Sci U S A. 92: 9151-5. PMID: 7568091
  7. 蛋白酶激活受体 2 和凝血酶受体的激动剂识别。细胞外环相互作用对受体功能的重要性。  |  Lerner, DJ., et al. 1996. J Biol Chem. 271: 13943-7. PMID: 8662993
  8. 人内皮细胞中的蛋白酶活化受体 2 受炎症介质诱导。与凝血酶受体的比较。  |  Nystedt, S., et al. 1996. J Biol Chem. 271: 14910-5. PMID: 8663011
  9. 人类蛋白酶活化受体-3（PAR-3）基因。在 Par 基因簇中进行鉴定，并在血管内皮细胞和血小板中进行表征。  |  Schmidt, VA., et al. 1998. J Biol Chem. 273: 15061-8. PMID: 9614115
  10. 人类蛋白酶激活受体 4 的克隆和特征描述。  |  Xu, WF., et al. 1998. Proc Natl Acad Sci U S A. 95: 6642-6. PMID: 9618465
  11. 凝血酶  |  Goldsack, NR., et al. 1998. Int J Biochem Cell Biol. 30: 641-6. PMID: 9695019
  12. 分析通过凝血酶受体途径介导超极化的 Ca2+ 激活的 K+ 通道。  |  Sullivan, R., et al. 1998. Am J Physiol. 275: C1342-8. PMID: 9814983

订购信息

产品名称产品编号规格价格数量收藏夹

PAR-2 抗体 (SAM11)

sc-13504
200 µg/ml
RMB2377.00

PAR-2 (SAM11): m-IgG Fc BP-HRP 套装

sc-525471
200 µg Ab; 10 µg BP
RMB2662.00

PAR-2 (SAM11): m-IgG2a BP-HRP 套装

sc-546273
200 µg Ab; 10 µg BP
RMB2662.00

PAR-2 抗体 (SAM11) L

sc-13504 L
200 µg/0.1 ml
RMB2377.00

PAR-2 抗体 (SAM11) AC

sc-13504 AC
500 µg/ml, 25% agarose
RMB3129.00

PAR-2 抗体 (SAM11) HRP

sc-13504 HRP
200 µg/ml
RMB2377.00

PAR-2 抗体 (SAM11) FITC

sc-13504 FITC
200 µg/ml
RMB2482.00

PAR-2 抗体 (SAM11) PE

sc-13504 PE
200 µg/ml
RMB2580.00

PAR-2 抗体 (SAM11) Alexa Fluor® 488

sc-13504 AF488
200 µg/ml
RMB2685.00

PAR-2 抗体 (SAM11) Alexa Fluor® 546

sc-13504 AF546
200 µg/ml
RMB2685.00

PAR-2 抗体 (SAM11) Alexa Fluor® 594

sc-13504 AF594
200 µg/ml
RMB2685.00

PAR-2 抗体 (SAM11) Alexa Fluor® 647

sc-13504 AF647
200 µg/ml
RMB2685.00

PAR-2 抗体 (SAM11) Alexa Fluor® 680

sc-13504 AF680
200 µg/ml
RMB2685.00

PAR-2 抗体 (SAM11) Alexa Fluor® 790

sc-13504 AF790
200 µg/ml
RMB2685.00

In which concentration is azide-free antibody used for neutralization studies (sc-13504 L)

Asked by: bkasp
Thank you for your question. This antibody is recommended for detection of PAR-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM and has not been tested in neutralization assays.
Answered by: Tech Support Europe
Date published: 2023-06-28

Was the antibody sc-13504 (SAM11) validated for rabbit tissue?

Asked by: Johanna_USZ
Thank you for your question. Anti-PAR-2 Antibody (SAM11) is recommended for detection of PAR-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. It has not been tested in rabbit cells or tissues so we do not have data on the efficacy of this antibody in such samples.
Answered by: Tech Support Europe
Date published: 2020-08-20

I would like to use the PAR-2 Antibody (SAM11): sc-13504 to double stain my tissue samples with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Yes, this is definitely possible. In this case, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, the PAR-2 Antibody (SAM11): sc-13504 is available conjugated to HRP (sc-13504 HRP), phycoerythrin (sc-13504 PE), fluoroscein (sc-13504 FITC), Alexa Fluor® 488 (sc-13504 AF488), Alexa Fluor® 647 (sc-13504 AF647), or Alexa Fluor® 405 (sc-13504 AF405, 100 µg/2 ml).
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 5 out of 5 by from robust bandThis antibody raised against the PAR-2,I tried diffenct conditions, 1:600 recommend for WB,band is very roubust.
Date published: 2017-06-24
Rated 5 out of 5 by from A good antibodyUsed this antibody in WB at 1:200 dilution, clear and strong.
Date published: 2017-04-05
Rated 5 out of 5 by from strong staining.Excellent results by IF, on mouse skeletal muscle 1:200,strong staining.
Date published: 2017-02-26
Rated 5 out of 5 by from good resultThis has worked well in IHC, 1:1000 dilution used for human stomach tissue.
Date published: 2017-02-12
Rated 5 out of 5 by from Excellent!This is an overall great antibody. Lots of publications, and works well in multiple applications. Solid choice.
Date published: 2016-12-27
Rated 5 out of 5 by from The AF488 conjugate of the antibody worksThe AF488 conjugate of the antibody works great in flow cytometry.
Date published: 2015-10-28
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human heart muscle tissue. -SCBT QC
Date published: 2015-05-20
Rated 4 out of 5 by from This antibody has worked well to detect PARThis antibody has worked well to detect PAR-2 in human samples.
Date published: 2015-04-03
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PAR-2 抗体 (SAM11) is rated 4.9 out of 5 by 10.
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