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pan-Cytokeratin Antibody (C11) - Western Blotting - Image 354292
pan-Cytokeratin 抗体 (C11) HRP: sc-8018 HRP. 直接 Western 印迹分析 MCF7 (A), HeLa (B), SW480 (C) 和 A549 (D) 全细胞裂解液中 pan-Cytokeratin 的表达.
pan-Cytokeratin 抗体 (C11) HRP: sc-8018 HRP. 直接 Western 印迹分析 MCF7 (A), HeLa (B), SW480 (C) 和 A549 (D) 全细胞裂解液中 pan-Cytokeratin 的表达.

pan-Cytokeratin 抗体 (C11): sc-8018

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说明书
  • pan-Cytokeratin 抗体 C11 是小鼠单克隆 IgG1 κ，pan-Cytokeratin抗体, 在164篇文献中引用，规格为200 µg/ml
  • 针对富含角蛋白的human表皮样癌细胞系A-431
  • pan-Cytokeratin 抗体 (C11) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 Cytokeratin 4, 5, 6, 8, 10, 13 and 18
  • 抗pan-Cytokeratin抗体（C11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取pan-Cytokeratin (C11): sc-8018的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 pan-Cytokeratin 抗体 (C11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与pan-Cytokeratin 抗体 (C11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Cytokeratin抗体，包括Basic Cytokeratin, Cytokeratin 1, Cytokeratin 10, Cytokeratin 12, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17和Cytokeratin 18

泛细胞角蛋白抗体（C11）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和 酶联免疫吸附测定（ELISA）。泛细胞角蛋白（C11）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合型。细胞角蛋白是一组多种中间丝状蛋白，对于维持上皮细胞的完整结构至关重要，在角化上皮组织和非角化上皮组织中成对表达。细胞角蛋白的重要性还体现在它们在细胞分化和组织特化中的作用，因此这些蛋白是识别和鉴定恶性肿瘤的有价值的标记物。例如，特定的细胞角蛋白（如细胞角蛋白10和细胞角蛋白13）在某些鳞状细胞癌中高度表达，而细胞角蛋白18主要存在于腺癌和基底细胞癌中。泛细胞角蛋白抗体（C11）对这些蛋白质的识别增强了其在癌症研究和诊断中的研究效用，为肿瘤生物学提供了见解，并有助于评估组织分化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

pan-Cytokeratin 抗体 (C11) 参考文献:

  1. K15 的表达意味着分层上皮基底细胞内的横向分化。  |  Porter, RM., et al. 2000. Lab Invest. 80: 1701-10. PMID: 11092530
  2. 使用链特异性抗体研究皮肤上皮囊肿和一些不常见的囊性肿瘤中细胞角蛋白的表达。  |  Broekaert, D., et al. 1990. Arch Dermatol Res. 282: 383-91. PMID: 1701984
  3. 人类肺部肿瘤：抗原谱与组织学类型的相关性。  |  Gatter, KC., et al. 1985. Histopathology. 9: 805-23. PMID: 2414203
  4. 两种单克隆抗角蛋白抗体的特性及其在上皮疾病研究中的应用。  |  Pulford, KA., et al. 1985. Histopathology. 9: 825-40. PMID: 2414204
  5. 头面部莫氏基底细胞癌快速冷冻切片免疫细胞化学的泛细胞角蛋白标记：确定首选标记的比较和评估。  |  Orchard, GE., et al. 2015. Br J Biomed Sci. 72: 61-6. PMID: 26126321
  6. [评估免疫组织化学泛细胞角蛋白和维多利亚蓝双重染色在评估pT3和pT4a结直肠癌分期中的作用]。  |  Xie, LW., et al. 2023. Zhonghua Bing Li Xue Za Zhi. 52: 1034-1036. PMID: 37805397
  7. 评估健康人和前列腺癌患者细胞角蛋白 8 和 18 片段的新型肿瘤标记物。  |  Silén, A., et al. 1994. Prostate. 24: 326-32. PMID: 7516071
  8. 用于定量检测健康人和癌症患者血清中细胞角蛋白 8 和 18 片段的新型 IRMA 和 ELISA。  |  Silén, A., et al. 1995. Scand J Clin Lab Invest. 55: 153-61. PMID: 7545308
  9. 正常和（恶性前）头颈部上皮细胞角蛋白的表达：综述。  |  van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025
  10. 肺癌患者血清和组织中细胞角蛋白 19（cyfra 21-1）片段的分布。  |  Quillien, V., et al. 1995. Anticancer Res. 15: 2857-63. PMID: 8669879
  11. 通过反义 RNA 使 p53 功能失活可诱导肺癌细胞的侵袭能力并下调细胞角蛋白的合成。  |  Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
  12. 肝细胞中细胞角蛋白的表达, 纤维组织和微妙功能。  |  Marceau, N. and Loranger, A. 1995. Biochem Cell Biol. 73: 619-25. PMID: 8714681

订购信息

产品名称产品编号规格价格数量收藏夹

pan-Cytokeratin 抗体 (C11)

sc-8018
200 µg/ml
RMB2377.00

pan-Cytokeratin (C11): m-IgG Fc BP-HRP 套装

sc-528202
200 µg Ab; 10 µg BP
RMB2662.00

pan-Cytokeratin (C11): m-IgGκ BP-HRP 套装

sc-520524
200 µg Ab, 40 µg BP
RMB2662.00

pan-Cytokeratin (C11): m-IgG1 BP-HRP 套装

sc-542813
200 µg Ab; 20 µg BP
RMB2662.00

pan-Cytokeratin 抗体 (C11) AC

sc-8018 AC
500 µg/ml, 25% agarose
RMB3129.00

pan-Cytokeratin 抗体 (C11) HRP

sc-8018 HRP
200 µg/ml
RMB2377.00

pan-Cytokeratin 抗体 (C11) FITC

sc-8018 FITC
200 µg/ml
RMB2482.00

pan-Cytokeratin 抗体 (C11) PE

sc-8018 PE
200 µg/ml
RMB2580.00

pan-Cytokeratin 抗体 (C11) Alexa Fluor® 488

sc-8018 AF488
200 µg/ml
RMB2685.00

pan-Cytokeratin 抗体 (C11) Alexa Fluor® 546

sc-8018 AF546
200 µg/ml
RMB2685.00

pan-Cytokeratin 抗体 (C11) Alexa Fluor® 594

sc-8018 AF594
200 µg/ml
RMB2685.00

pan-Cytokeratin 抗体 (C11) Alexa Fluor® 647

sc-8018 AF647
200 µg/ml
RMB2685.00

pan-Cytokeratin 抗体 (C11) Alexa Fluor® 680

sc-8018 AF680
200 µg/ml
RMB2685.00

pan-Cytokeratin 抗体 (C11) Alexa Fluor® 790

sc-8018 AF790
200 µg/ml
RMB2685.00

I use SC-8018-AF488 for FACS analysis of 4T1 breast cancer primary cell. Do yo think a isotype control is needed? Do you have a isotype control? Thanks.

Asked by: Yuan2018
Thank you for your question. We always recommend using an isotype control for FCM and FACS analysis. This pan-Cytokeratin Antibody (C11) is a mouse monoclonal IgG1, so the recommended isotype control for your purpose is normal mouse IgG1 Alexa Fluor® 488: sc-3890.
Answered by: Technical Support
Date published: 2019-04-26

Can pan-Cytokeratin Antibody (C11): sc-8018 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, pan-Cytokeratin Antibody (C11): sc-8018 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 5 out of 5 by from Pan-cytokeratin Ab for IFVery good antibody, dilution 1:1000 Paraffin samples Stains the differentiated cells of mouse/human tissue
Date published: 2021-03-23
Rated 5 out of 5 by from C11 AntibodyUtilized for staining cytokeratins in rabbit corneal epithelial cells.
Date published: 2017-07-17
Rated 5 out of 5 by from Good WBFor WB it's really good, I got a very good band!!!
Date published: 2017-05-13
Rated 5 out of 5 by from is goodClear and strong use dilution 1:1000, very happy result
Date published: 2017-04-22
Rated 5 out of 5 by from Works well for IHCHas good signal for paraffin-embedded samples for fluorescent immunohistochemistry.
Date published: 2017-03-30
Rated 4 out of 5 by from a clean antibodyA non-specific band at 20 kDa, otherwise a clean antibody
Date published: 2017-01-25
Rated 5 out of 5 by from Great Cytokeratin antibodyAn easy choice for cytokeratin detection. It will react with multiple isoforms and several mammalian species. Provides clear and strong signals. This antibody has been published with nearly 30 times.
Date published: 2017-01-13
Rated 5 out of 5 by from Wow!! Hard to beatIHC staining as good as it gets; also very strong WB
Date published: 2017-01-06
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pan-Cytokeratin 抗体 (C11) is rated 4.9 out of 5 by 14.
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