Date published: 2026-10-20

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pan-Cytokeratin Antibody (AE1/AE3) - Western Blotting - Image 360889
泛细胞角蛋白抗体 (AE1/AE3): sc-81714. HeLa (A), HEK293 (B), Neuro-2A (C) 和 KNRK (D) 全细胞裂解液中 pan-Cytokeratin 表达的 Western 印迹分析.
泛细胞角蛋白抗体 (AE1/AE3): sc-81714. HeLa (A), HEK293 (B), Neuro-2A (C) 和 KNRK (D) 全细胞裂解液中 pan-Cytokeratin 表达的 Western 印迹分析.

pan-Cytokeratin 抗体 (AE1/AE3): sc-81714

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寫評論提問
说明书
  • pan-Cytokeratin抗体(AE1/AE3)是小鼠单克隆IgG1 κ, 在76篇文献中引用，规格为200 µg/ml
  • 针对表皮角蛋白的 human 起源
  • 推荐用于 mouse, rat, human, bovine 和 rabbit 来源的most acidic (type I) and basic (type II) Cytokeratins WB, IP, IF 和 IHC(P)检测
  • 另提供HRP 偶联抗体，可用于WB, IHC(P)和ELISA实验
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是pan-Cytokeratin Antibody (AE1/AE3) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与pan-Cytokeratin Antibody (AE1/AE3) 打包提供（请参阅下面的订购信息）。
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说明书与实验方案
研究信息

関連項目

附加Cytokeratin抗体，包括Basic Cytokeratin, Cytokeratin 1, Cytokeratin 10, Cytokeratin 12, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17, Cytokeratin 18, Cytokeratin 19, Cytokeratin 20, Cytokeratin 23, Cytokeratin 2E, Cytokeratin 2p和Cytokeratin 3

泛细胞角蛋白抗体（AE1/AE3）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过应用诸如蛋白质印迹（WB）、免疫 免疫沉淀（IP）、免疫荧光（IF）以及石蜡包埋切片免疫组织化学（IHCP）等方法检测。泛细胞角蛋白抗体（AE1/AE3）有结合和非结合两种形式，可满足各种实验需求。泛细胞角蛋白单克隆抗体（AE1/AE3）可识别的细胞角蛋白是一组重要的中间丝状蛋白，可维持上皮细胞的完整结构。这些蛋白质成对表达，对于细胞分化和组织分化至关重要，因此在癌症研究中具有特殊价值。特定的细胞角蛋白是区分不同类型肿瘤的可靠生物标记；细胞角蛋白10和13在某些鳞状细胞癌中经常升高，而细胞角蛋白18主要在腺癌和基底细胞癌中表达。细胞角蛋白的表达模式有助于确定恶性肿瘤的特征，并深入了解上皮细胞的功能和病理。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

pan-Cytokeratin 抗体 (AE1/AE3) 参考文献:

  1. 羊食管上皮细胞培养和体外食管组织工程。  |  Saxena, AK., et al. 2010. Tissue Eng Part C Methods. 16: 109-14. PMID: 19374530
  2. 免疫组织化学在胸膜肉瘤样间皮瘤与肺肉瘤样癌鉴别诊断中的价值。  |  Takeshima, Y., et al. 2009. Histopathology. 54: 667-76. PMID: 19438742
  3. 原发性鼻-鼻窦黑色素瘤的免疫表型异质性与神经内分泌标志物和钙粘蛋白的异常表达。  |  Lee, H., et al. 2011. Appl Immunohistochem Mol Morphol. 19: 48-53. PMID: 20881840
  4. 下颌下唾液腺小细胞癌的极罕见病例。  |  Philip, NR., et al. 2017. J Clin Diagn Res. 11: ED24-ED25. PMID: 28658784
  5. 病理切片数据整合，用于肿瘤免疫细胞浸润中多种细胞类型的定量成像。  |  Ma, Z., et al. 2017. Diagn Pathol. 12: 69. PMID: 28923066
  6. 同种异体移植炎症因子-1在原发性腹膜间皮细胞炎症和氧化应激调节中的作用。  |  Zhou, Y., et al. 2019. Cell Biol Int. 43: 495-505. PMID: 30761680
  7. 细胞系衍生异种移植小鼠模型是研究结直肠癌肿瘤分化的合适体内模型。  |  Georges, LMC., et al. 2019. Front Med (Lausanne). 6: 139. PMID: 31316988
  8. 分期支气管内超声引导下经支气管针吸术检测的非小细胞肺癌淋巴结微转移的发生率和临床意义。  |  Belanger, AR., et al. 2019. J Thorac Dis. 11: 3650-3658. PMID: 31559073
  9. 无AKT1、HRAS或PIK3CA突变的肺上皮-肌上皮癌：病例报告。  |  Yanagawa, N., et al. 2020. Diagn Pathol. 15: 105. PMID: 32859224
  10. 腹膜后肿瘤病例显示上皮分化、明显的粘液样基质和INI1/SMARCB1缺失。  |  Rekhi, B. and Thomas, A. 2021. Pathologica. 113: 456-462. PMID: 34974552

订购信息

产品名称产品编号规格价格数量收藏夹

pan-Cytokeratin 抗体 (AE1/AE3)

sc-81714
200 µg/ml
CNY2422.00

pan-Cytokeratin (AE1/AE3): m-IgG Fc BP-HRP 套装

sc-528712
200 µg Ab; 10 µg BP
CNY2715.00

pan-Cytokeratin (AE1/AE3): m-IgGκ BP-HRP 套装

sc-521181
200 µg Ab, 40 µg BP
CNY2715.00

pan-Cytokeratin (AE1/AE3): m-IgG1 BP-HRP 套装

sc-543082
200 µg Ab; 20 µg BP
CNY2715.00

pan-Cytokeratin 抗体 (AE1/AE3) HRP

sc-81714 HRP
200 µg/ml
CNY2422.00

Can i use this for bovine mammary tissue

Asked by: Qamar
Thank you for your question. The antibody is recommended for mouse, rat, human, bovine, and rabbit
Answered by: BlakeJ
Date published: 2026-01-05

Can I apply this product to canine urothelium

Asked by: Wan PJ
Thank you for your question. sc-81714 is recommended for the detection of pan-Cytokeratin of mouse, rat, human, bovine and rabbit origin.
Answered by: Tech Support Europe
Date published: 2022-03-17

Can I use this antibody in IF against primary cells collected from a mouse to detect pan CK?

Asked by: aroberts
Thank you for your question. Yes, this mouse monoclonal antibody, pan-Cytokeratin (AE1/AE3): sc-81714, is recommended for IF in mouse cells.
Answered by: Technical Support
Date published: 2017-11-15

I no longer have the goat anti-mouse secondary I was using. What secondary reagent do you recommend?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 5 out of 5 by Paloma from Pan-cytokeratin Ab for IFExcellent antibody dilution 1:1000 paraffin sections fixation:paraformaldehyde
Date published: 2021-03-23
Rated 5 out of 5 by Ratatouille298 from Very good antibodyGreat staining with paraffin-embedded human and rabbit skin at 1/1000 dilution
Date published: 2019-05-17
Rated 5 out of 5 by Dhiman from strong signal in western blottested this in HCC cell lines. works great by western blot
Date published: 2018-04-03
Rated 5 out of 5 by Histologistics from Works with Human skinWe got this as a sample to test with our tissues. We used human skin at a concentration of 1:250 and found it stained very well.
Date published: 2017-09-22
Rated 5 out of 5 by scriswel from Very good antibodyI used this antibody on formalin-fixed paraffin embedded human squamous carcinoma tissues at a 1:30 dilution for 15 minutes. Staining was vibrant and crisp.
Date published: 2017-08-10
Rated 5 out of 5 by SERENA from all goodThe study found that cytokeratin is a useful marker of tissue differentiation, which can be directly applied to the characterization of malignant tumors. For example, cytokeratin 10 and 13 are highly expressed in subgroups of squamous cell carcinomas, whereas cytokeratin 18 is expressed in most adenocarcinomas and basal cell carcinomas
Date published: 2017-02-08
Rated 5 out of 5 by Bill28 from Great productVery nice data in several applications including if ,ihc and wb
Date published: 2017-01-02
Rated 5 out of 5 by Cweed from Great protein recognition!SC-81714 is a great choice for cytokeratin detection. Its ability to recognize both acidic and basic subfamily members results in fantastic staining. This antibody has been featured in more than 15 publications.
Date published: 2016-12-16
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pan-Cytokeratin 抗体 (AE1/AE3) is rated 5.0 out of 5 by 11.
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