Date published: 2025-9-8
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pan 14-3-3 Antibody (H-8) - Western Blotting - Image 354296
泛 14-3-3 抗体 (H-8) HRP: sc-1657 HRP. HeLa (A), 293 (B), K-562 (C) 和 Jurkat (D) 全细胞裂解液中 pan 14-3-3 表达的直接 Western 印迹分析.
泛 14-3-3 抗体 (H-8) HRP: sc-1657 HRP. HeLa (A), 293 (B), K-562 (C) 和 Jurkat (D) 全细胞裂解液中 pan 14-3-3 表达的直接 Western 印迹分析.

pan 14-3-3 抗体 (H-8): sc-1657

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说明书
  • pan 14-3-3抗体(H-8)是小鼠单克隆IgG2b κ, 在239篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的pan 14-3-3的N-terminus的氨基酸1-30之间
  • pan 14-3-3 抗体 (H-8) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA ，检测 mouse, rat, human 和 来源的pan 14-3-3; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • pan 14-3-3抗体（H-8）可与HRP结合用于WB、IHC(P)和ELISA；与phycoerythrin或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-1657 X, 200 µg/ml)
  • 关于如何获取pan 14-3-3 (H-8): sc-1657的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 pan 14-3-3 抗体 (H-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与pan 14-3-3 抗体 (H-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外14-3-3抗体，包括14-3-3 β, 14-3-3 ε, 14-3-3 η, 14-3-3 γ, 14-3-3 σ, 14-3-3 θ, 14-3-3 ζ和pan 14-3-3

pan 14-3-3抗体（H-8）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织 化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）。泛14-3-3单克隆抗体（H-8）有结合和非结合两种形式，可满足各种实验需求。14-3-3蛋白家族在调节多种细胞过程方面发挥着关键作用，尤其是与癌症生物学相关的过程，如细胞凋亡、促有丝分裂信号和细胞周期检查点。该家族由7种同源物组成，包括14-3-3β、γ、ε、ζ、η、θ和σ，它们形成二聚体，为配体蛋白创造两个结合位点，促进原本不会发生的相互作用。14-3-3蛋白通过细胞质隔离、相互作用域调节和防止降解等机制参与调节其配体蛋白。这些蛋白与各种靶蛋白相互作用，包括激酶和磷酸酶，它们对于维持细胞内稳态和信号通路至关重要。14-3-3蛋白能够与磷酸化丝氨酸/苏氨酸残基结合，这凸显了它们在细胞信号传导中的重要性，因为其表达或功能的改变会导致包括癌症在内的多种病理状况。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

pan 14-3-3 抗体 (H-8) 参考文献:

  1. DAL-1/蛋白 4.1B 肿瘤抑制因子的 4.1/ezrin/radixin/moesin 结构域与 14-3-3 蛋白相互作用。  |  Yu, T., et al. 2002. Biochem J. 365: 783-9. PMID: 11996670
  2. MADM 是一种新型适配蛋白，可介导髓系白血病因子 1 的 14-3-3 结合位点发生磷酸化。  |  Lim, R., et al. 2002. J Biol Chem. 277: 40997-1008. PMID: 12176995
  3. 14-3-3 癌症联系  |  Hermeking, H. 2003. Nat Rev Cancer. 3: 931-43. PMID: 14737123
  4. 拟南芥中 14-3-3 蛋白的同工酶特异性亚细胞定位似乎是由客户相互作用驱动的。  |  Paul, AL., et al. 2005. Mol Biol Cell. 16: 1735-43. PMID: 15659648
  5. 14-3-3抑制可促进多巴胺能神经元的丢失，而14-3-3θ的过度表达可促进帕金森病MPTP小鼠模型的恢复。  |  Ding, H., et al. 2015. Neuroscience. 307: 73-82. PMID: 26314634
  6. 14-3-3蛋白调节突变型LRRK2激酶活性和神经突触缩短。  |  Lavalley, NJ., et al. 2016. Hum Mol Genet. 25: 109-22. PMID: 26546614
  7. 14-3-3蛋白在路易体或阿尔茨海默病神经退行性疾病中的失调。  |  McFerrin, MB., et al. 2017. Ann Clin Transl Neurol. 4: 466-477. PMID: 28695147
  8. hsa_circ_0001546/14-3-3/CAMK2D/Tau复合体驱动的Tau聚集依赖性过氧化脂质积累抑制上皮性卵巢癌腹膜转移。  |  Chai, B., et al. 2024. Adv Sci (Weinh). 11: e2310134. PMID: 38634567
  9. 14-3-3：信号蛋白的调节剂？  |  Morrison, D. 1994. Science. 266: 56-7. PMID: 7939645
  10. 人类 14-3-3 eta 链基因（YWHAH）的结构组织和染色体分配。  |  Muratake, T., et al. 1996. Genomics. 36: 63-9. PMID: 8812417
  11. 14-3-3 与磷酸肽结合特异性的结构基础。  |  Yaffe, MB., et al. 1997. Cell. 91: 961-71. PMID: 9428519
  12. 一种新型鞘氨醇依赖性蛋白激酶（SDK1）能特异性地使 14-3-3 蛋白的某些同工型发生磷酸化。  |  Megidish, T., et al. 1998. J Biol Chem. 273: 21834-45. PMID: 9705322

订购信息

产品名称产品编号规格价格数量收藏夹

pan 14-3-3 抗体 (H-8)

sc-1657
200 µg/ml
RMB2377.00

pan 14-3-3 (H-8): m-IgGκ BP-HRP 套装

sc-520434
200 µg Ab, 40 µg BP
RMB2662.00

pan 14-3-3 (H-8): m-IgG2b BP-HRP 套装

sc-548828
200 µg Ab; 10 µg BP
RMB2662.00

pan 14-3-3 抗体 (H-8) X

sc-1657 X
200 µg/ml
RMB2377.00

pan 14-3-3 抗体 (H-8) HRP

sc-1657 HRP
200 µg/ml
RMB2377.00

pan 14-3-3 抗体 (H-8) FITC

sc-1657 FITC
200 µg/ml
RMB2482.00

pan 14-3-3 抗体 (H-8) PE

sc-1657 PE
200 µg/ml
RMB2580.00

pan 14-3-3 抗体 (H-8) Alexa Fluor® 488

sc-1657 AF488
200 µg/ml
RMB2685.00

pan 14-3-3 抗体 (H-8) Alexa Fluor® 546

sc-1657 AF546
200 µg/ml
RMB2685.00

pan 14-3-3 抗体 (H-8) Alexa Fluor® 594

sc-1657 AF594
200 µg/ml
RMB2685.00

pan 14-3-3 抗体 (H-8) Alexa Fluor® 647

sc-1657 AF647
200 µg/ml
RMB2685.00

pan 14-3-3 抗体 (H-8) Alexa Fluor® 680

sc-1657 AF680
200 µg/ml
RMB2685.00

pan 14-3-3 抗体 (H-8) Alexa Fluor® 790

sc-1657 AF790
200 µg/ml
RMB2685.00

pan 14-3-3 抗体 (H-8) TRITC

sc-1657 TRITC
200 µg/ml
RMB2407.00

pan 14-3-3 (H-8) 中和勝肽

sc-1657 P
100 µg/0.5 ml
RMB511.00

Hi, I would like to know if this pan 14.3.3 is available without BSA and gelatin? Thank' for replying

Asked by: PhL6900
Thank you for your question. Please contact our technical service team to inquire directly about available custom formulations.
Answered by: Tech Support Europe
Date published: 2022-03-09

what is the recommended shelf life of Ab. should I freeze it for long tern storage

Asked by: dg001
Thank you for your question. For long term storage we recommend to stock our Abs at 4°C. Please contact technical service if you need additional information.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

How should I go about IHC staining with the pan 14-3-3 Antibody (H-8) if I am working with mouse tissue samples?

Asked by: Trav11
In this case, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, the pan 14-3-3 Antibody (H-8): sc-1657 is available conjugated to HRP (sc-1657 HRP), phycoerythrin (sc-1657 PE), fluoroscein (sc-1657 FITC), Alexa Fluor® 488 (sc-1657 AF488), Alexa Fluor® 647 (sc-1657 AF647), TRITC (sc-1657 TRITC, 200 µg/ml) or Alexa Fluor® 405 (sc-1657 AF405, 100 µg/2 ml).
Answered by: Tech Service 8
Date published: 2016-12-20

What secondary reagent should I use to detect pan 14-3-3 Antibody (H-8) via Western blot in human samples?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-20
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Rated 5 out of 5 by from the best 14-3-3 antibodyWe obtained a sample vial of this antibody and it has been working well. We are now buying a full size!
Date published: 2025-04-26
Rated 5 out of 5 by from Very good in WBVery good ab with virtually no background for WB detection of the endogenous human 14-3-3 proteins.
Date published: 2022-05-20
Rated 5 out of 5 by from 14-3-3 antibody (H-8)This antibody works well in immunoblots, with strong signal across multiple subtypes and minimal background
Date published: 2021-07-30
Rated 4 out of 5 by from Ottimo funzionamentoAnticorpo utilizzato secondo indicazioni del datasheet. Perfettamente funzionante.
Date published: 2020-10-06
Rated 5 out of 5 by from GoodA band of predicted size was detected in WB (HEK293 and mouse brain lysate).
Date published: 2020-06-26
Rated 5 out of 5 by from Works for WB and IPWorks excellently for WB (1:2500) and IP. Recommended!
Date published: 2017-12-26
Rated 5 out of 5 by from excellent antibodyUsed this in 1:500 dilution in 5% BSA on an old nitrocellulose blot and still detected the 14-3-3 protein at the right band size and results were reproducable. Is one of the working pan 14-3-3 antibodies in the market I believe.
Date published: 2017-08-16
Rated 5 out of 5 by from Good antibodyAntibody gives great results by Western Blot. Clear and strong band at expected size without any additional bands. Cytoplasmic bright staining by IF was observed after methanol fixation. We tested antibody on MDCKII, mouse collecting duct and aortic cell lines.
Date published: 2017-04-19
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pan 14-3-3 抗体 (H-8) is rated 4.9 out of 5 by 16.
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