Date published: 2026-10-20

1-800-457-3801

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
pan 14-3-3 Antibody (B-8) - Western Blotting - Image 352778
泛 14-3-3 抗体 (B-8): sc-133233. K-562 (A), Ramos (B), NAMALWA (C), NCI-H1299 (D), SK-N-MC (E) 和 PC-12 (F) 全细胞裂解液中 pan 14-3-3 表达的 Western 印迹分析.
泛 14-3-3 抗体 (B-8): sc-133233. K-562 (A), Ramos (B), NAMALWA (C), NCI-H1299 (D), SK-N-MC (E) 和 PC-12 (F) 全细胞裂解液中 pan 14-3-3 表达的 Western 印迹分析.

pan 14-3-3 抗体 (B-8): sc-133233

4.7(12)
寫評論提問
说明书
  • pan 14-3-3 抗体 B-8 是小鼠单克隆 IgG1 κ，pan 14-3-3抗体, 在14篇文献中引用，规格为200 µg/ml
  • 与氨基酸1-246结合，代表全长14-3-3 β，human起源
  • pan 14-3-3 抗体 (B-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 pan 14-3-3
  • 抗pan 14-3-3抗体（B-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是pan 14-3-3 Antibody (B-8) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与pan 14-3-3 Antibody (B-8) 打包提供（请参阅下面的订购信息）。
Gene Editing Promo Banner

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外14-3-3抗体，包括14-3-3 β, 14-3-3 ε, 14-3-3 η, 14-3-3 γ, 14-3-3 σ, 14-3-3 θ, 14-3-3 ζ和pan 14-3-3

pan 14-3-3抗体（B-8）是一种IgG1 κ小鼠单克隆pan 14-3-3抗体（也称为14-3-3蛋白质抗体，或酪氨酸3-单加氧酶/色氨酸5-单加氧酶激活蛋白家族抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的pan 14-3-3蛋白质。pan 14-3-3抗体（B-8）既可以是非偶联的抗pan 14-3-3抗体形式，也可以是多种偶联形式的抗pan 14-3-3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。14-3-3蛋白质调节许多与癌症生物学相关的细胞过程，特别是凋亡、有丝分裂信号和细胞周期检查点。由七个异构体组成的信号中间体家族，称为14-3-3 β、γ、ε、ζ、η、θ和Σ。14-3-3蛋白质形成二聚体，为配体蛋白质提供两个结合位点，从而将两个原本可能不会结合的蛋白质结合在一起。这些配体在很大程度上共享一个14-3-3共识结合基序，并表现出丝氨酸/苏氨酸磷酸化。14-3-3蛋白质通过细胞质隔离、相互作用域和输入/输出序列的占据、降解的预防、酶活性的激活/抑制以及蛋白质修饰的促进，对这些配体蛋白质进行广泛的调节。因此，其表达的丧失会导致大量的致病细胞活动。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

pan 14-3-3 抗体 (B-8) 参考文献:

  1. 药物诱导的棘球蚴原头节中热休克蛋白和14-3-3蛋白的分子和生化挖掘以及抗蠕虫药抗性候选基因的检测。  |  Pan, D., et al. 2011. J Helminthol. 85: 196-203. PMID: 20735897
  2. 家族性帕金森病的常见突变会削弱磷酸化依赖性14-3-3与LRRK2的结合。  |  Li, X., et al. 2011. PLoS One. 6: e17153. PMID: 21390248
  3. 14-3-3抑制可促进多巴胺能神经元的丢失，而14-3-3θ的过度表达可促进帕金森病MPTP小鼠模型的恢复。  |  Ding, H., et al. 2015. Neuroscience. 307: 73-82. PMID: 26314634
  4. 14-3-3蛋白调节突变型LRRK2激酶活性和神经突触缩短。  |  Lavalley, NJ., et al. 2016. Hum Mol Genet. 25: 109-22. PMID: 26546614
  5. 14-3-3蛋白在路易体或阿尔茨海默病神经退行性疾病中的失调。  |  McFerrin, MB., et al. 2017. Ann Clin Transl Neurol. 4: 466-477. PMID: 28695147
  6. 柑橘14-3-3基因的分子分析及其对不同压力的反应  |  Lyu, S., et al. 2021. Int J Mol Sci. 22: PMID: 33430069
  7. 依布硒酮对半胱氨酸的修饰降低了14-3-3蛋白的稳定性和细胞水平。  |  Waløen, K., et al. 2021. Mol Pharmacol. 100: 155-169. PMID: 34031189
  8. 植物14-3-3蛋白的分子进化以及Hv14-3-3A在气孔调节和耐旱性方面的功能。  |  Jiang, W., et al. 2023. Plant Cell Physiol. 63: 1857-1872. PMID: 35323970
  9. hsa_circ_0001546/14-3-3/CAMK2D/Tau复合体驱动的Tau聚集依赖性过氧化脂质积累抑制上皮性卵巢癌腹膜转移。  |  Chai, B., et al. 2024. Adv Sci (Weinh). 11: e2310134. PMID: 38634567
  10. 14-3-3蛋白的PAR化控制了Magnaporthe oryzae的毒力。  |  Gao, X., et al. 2024. Nat Commun. 15: 8047. PMID: 39277621

订购信息

产品名称产品编号规格价格数量收藏夹

pan 14-3-3 抗体 (B-8)

sc-133233
200 µg/ml
CNY2422.00

pan 14-3-3 (B-8): m-IgG Fc BP-HRP 套装

sc-528778
200 µg Ab; 10 µg BP
CNY2715.00

pan 14-3-3 (B-8): m-IgGκ BP-HRP 套装

sc-521285
200 µg Ab, 40 µg BP
CNY2715.00

pan 14-3-3 (B-8): m-IgG1 BP-HRP 套装

sc-543122
200 µg Ab; 20 µg BP
CNY2715.00

pan 14-3-3 抗体 (B-8) AC

sc-133233 AC
500 µg/ml, 25% agarose
CNY3189.00

pan 14-3-3 抗体 (B-8) HRP

sc-133233 HRP
200 µg/ml
CNY2422.00

pan 14-3-3 抗体 (B-8) FITC

sc-133233 FITC
200 µg/ml
CNY2527.00

pan 14-3-3 抗体 (B-8) PE

sc-133233 PE
200 µg/ml
CNY2625.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 488

sc-133233 AF488
200 µg/ml
CNY2738.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 546

sc-133233 AF546
200 µg/ml
CNY2738.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 594

sc-133233 AF594
200 µg/ml
CNY2738.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 647

sc-133233 AF647
200 µg/ml
CNY2738.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 680

sc-133233 AF680
200 µg/ml
CNY2738.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 790

sc-133233 AF790
200 µg/ml
CNY2738.00

What blocking solution should I use with pan 14-3-3 (B-8): sc-133233 monoclonal antibody for Western blot?

Asked by: jenni
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 15
Date published: 2017-02-09
  • y_2026, m_10, d_18, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_133233, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 87ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by STneuro from GoodA band of predicted size was detected in WB (HEK293 and mouse brain lysate).
Date published: 2020-06-26
Rated 5 out of 5 by woojonglee0917 from Worked great for human macorphagesWorked great for human macrophages at 1:100 dilution.
Date published: 2019-12-04
Rated 5 out of 5 by CBizzle from Great signal to noise by westernWork on human (293t) cell, and mouse/rat brain lysate. Nice bright band a bit above 25 kd (brighter ladder band-lane1). Shown, 25ug of rat brain lysate (lane2).
Date published: 2017-05-29
Rated 5 out of 5 by CBizzle from Strong specific band by western blotThis antibody works great on western blots using mouse, rat, or human protein samples. Nice, bright, specific band a little over 25 kDa.
Date published: 2017-05-10
Rated 3 out of 5 by dachiwo from worked against only betamyc-14-3-3 b, g, e, z were overexpressed. however only beta was detected. endogenous in 293 cells also detected. but I guess it is beta. 1/1000 dil in 2% BSA TBST, overnight rxn.
Date published: 2017-03-22
Rated 5 out of 5 by Just15 from Works wellWorks well for WB. Tested at 1:1000. Happy with the antibody.
Date published: 2017-03-06
Rated 5 out of 5 by Cweed from Great detection.This antibody will provide great pan 14-3-3 detect in mouse, rat, or human samples. It has great staining data and provides clear and specific WB.
Date published: 2017-01-25
Rated 5 out of 5 by abcd1234 from Excellent for WBReceived a sample for this antibody. Tried it on MEF and HEK-293. Worked very nicely at 1:1000 dilution. Single band detected and strong signal.
Date published: 2017-01-04
  • y_2026, m_10, d_19, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_133233, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
pan 14-3-3 抗体 (B-8) is rated 4.7 out of 5 by 12.
  • y_2026, m_10, d_19, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_133233, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 108ms
  • REVIEWS, PRODUCT
1-800-457-3801
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打1-800-457-3801联系我们。
版权所有© 2007-2026 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。