Date published: 2025-9-7
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pan 14-3-3 Antibody (B-8) - Western Blotting - Image 352778
泛 14-3-3 抗体 (B-8): sc-133233. K-562 (A), Ramos (B), NAMALWA (C), NCI-H1299 (D), SK-N-MC (E) 和 PC-12 (F) 全细胞裂解液中 pan 14-3-3 表达的 Western 印迹分析.
泛 14-3-3 抗体 (B-8): sc-133233. K-562 (A), Ramos (B), NAMALWA (C), NCI-H1299 (D), SK-N-MC (E) 和 PC-12 (F) 全细胞裂解液中 pan 14-3-3 表达的 Western 印迹分析.

pan 14-3-3 抗体 (B-8): sc-133233

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寫評論提問

说明书
  • pan 14-3-3 抗体 B-8 是小鼠单克隆 IgG1 κ，pan 14-3-3抗体, 在14篇文献中引用，规格为200 µg/ml
  • 与氨基酸1-246结合，代表全长14-3-3 β，human起源
  • pan 14-3-3 抗体 (B-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 pan 14-3-3
  • 抗pan 14-3-3抗体（B-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取pan 14-3-3 (B-8): sc-133233的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 pan 14-3-3 抗体 (B-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与pan 14-3-3 抗体 (B-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 14-3-3 抗体 包括 14-3-3 β, 14-3-3 ε, 14-3-3 η, 14-3-3 γ, 14-3-3 σ, 14-3-3 θ, 14-3-3 ζ和pan 14-3-3

pan 14-3-3抗体（B-8）是一种IgG1 κ小鼠单克隆pan 14-3-3抗体（也称为14-3-3蛋白质抗体，或酪氨酸3-单加氧酶/色氨酸5-单加氧酶激活蛋白家族抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的pan 14-3-3蛋白质。pan 14-3-3抗体（B-8）既可以是非偶联的抗pan 14-3-3抗体形式，也可以是多种偶联形式的抗pan 14-3-3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。14-3-3蛋白质调节许多与癌症生物学相关的细胞过程，特别是凋亡、有丝分裂信号和细胞周期检查点。由七个异构体组成的信号中间体家族，称为14-3-3 β、γ、ε、ζ、η、θ和Σ。14-3-3蛋白质形成二聚体，为配体蛋白质提供两个结合位点，从而将两个原本可能不会结合的蛋白质结合在一起。这些配体在很大程度上共享一个14-3-3共识结合基序，并表现出丝氨酸/苏氨酸磷酸化。14-3-3蛋白质通过细胞质隔离、相互作用域和输入/输出序列的占据、降解的预防、酶活性的激活/抑制以及蛋白质修饰的促进，对这些配体蛋白质进行广泛的调节。因此，其表达的丧失会导致大量的致病细胞活动。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

pan 14-3-3 抗体 (B-8)

sc-133233
200 µg/ml
RMB2377.00

pan 14-3-3 (B-8): m-IgG Fc BP-HRP 套装

sc-528778
200 µg Ab; 10 µg BP
RMB2662.00

pan 14-3-3 (B-8): m-IgGκ BP-HRP 套装

sc-521285
200 µg Ab, 40 µg BP
RMB2662.00

pan 14-3-3 (B-8): m-IgG1 BP-HRP 套装

sc-543122
200 µg Ab; 20 µg BP
RMB2662.00

pan 14-3-3 抗体 (B-8) AC

sc-133233 AC
500 µg/ml, 25% agarose
RMB3129.00

pan 14-3-3 抗体 (B-8) HRP

sc-133233 HRP
200 µg/ml
RMB2377.00

pan 14-3-3 抗体 (B-8) FITC

sc-133233 FITC
200 µg/ml
RMB2482.00

pan 14-3-3 抗体 (B-8) PE

sc-133233 PE
200 µg/ml
RMB2580.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 488

sc-133233 AF488
200 µg/ml
RMB2685.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 546

sc-133233 AF546
200 µg/ml
RMB2685.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 594

sc-133233 AF594
200 µg/ml
RMB2685.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 647

sc-133233 AF647
200 µg/ml
RMB2685.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 680

sc-133233 AF680
200 µg/ml
RMB2685.00

pan 14-3-3 抗体 (B-8) Alexa Fluor® 790

sc-133233 AF790
200 µg/ml
RMB2685.00

What blocking solution should I use with pan 14-3-3 (B-8): sc-133233 monoclonal antibody for Western blot?

Asked by: jenni
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 15
Date published: 2017-02-09
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Rated 4 out of 5 by from GoodA band of predicted size was detected in WB (HEK293 and mouse brain lysate).
Date published: 2020-06-26
Rated 5 out of 5 by from Worked great for human macorphagesWorked great for human macrophages at 1:100 dilution.
Date published: 2019-12-04
Rated 5 out of 5 by from Great signal to noise by westernWork on human (293t) cell, and mouse/rat brain lysate. Nice bright band a bit above 25 kd (brighter ladder band-lane1). Shown, 25ug of rat brain lysate (lane2).
Date published: 2017-05-29
Rated 5 out of 5 by from Strong specific band by western blotThis antibody works great on western blots using mouse, rat, or human protein samples. Nice, bright, specific band a little over 25 kDa.
Date published: 2017-05-10
Rated 3 out of 5 by from worked against only betamyc-14-3-3 b, g, e, z were overexpressed. however only beta was detected. endogenous in 293 cells also detected. but I guess it is beta. 1/1000 dil in 2% BSA TBST, overnight rxn.
Date published: 2017-03-22
Rated 5 out of 5 by from Works wellWorks well for WB. Tested at 1:1000. Happy with the antibody.
Date published: 2017-03-06
Rated 5 out of 5 by from Great detection.This antibody will provide great pan 14-3-3 detect in mouse, rat, or human samples. It has great staining data and provides clear and specific WB.
Date published: 2017-01-25
Rated 5 out of 5 by from Excellent for WBReceived a sample for this antibody. Tried it on MEF and HEK-293. Worked very nicely at 1:1000 dilution. Single band detected and strong signal.
Date published: 2017-01-04
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pan 14-3-3 抗体 (B-8) is rated 4.7 out of 5 by 12.
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