Date published: 2025-9-6
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PAC-1 Antibody (4O21) - Western Blotting - Image 10834
PAC-1 抗体 (4O21): sc-32776. 拉莫斯全细胞裂解液中 PAC1 表达的 Western 印迹分析.
PAC-1 Antibody (4O21) - Western Blotting - Image 10834
PAC-1 抗体 (4O21): sc-32776. 拉莫斯全细胞裂解液中 PAC1 表达的 Western 印迹分析.
PAC-1 Antibody (4O21) - Immunohistochemistry - Image 37673
PAC-1 抗体 (4O21): sc-32776. 福尔马林固定, 石蜡包埋的人侧脑室组织的免疫过氧化物酶染色, 低倍 (A) 和高倍 (B) 放大后显示神经元细胞的胞浆染色. 瑞典人类蛋白质图谱 (HPA) 计划友情提供.
PAC-1 Antibody (4O21) - Western Blotting - Image 14287
PAC-1 抗体 (4O21): sc-32776. 对 HeLa 全细胞裂解液中 PAC1 的表达进行 Western 印迹分析.
PAC-1 抗体 (4O21): sc-32776. 拉莫斯全细胞裂解液中 PAC1 表达的 Western 印迹分析.

PAC-1 抗体 (4O21): sc-32776

4.8(8)
寫評論提問

说明书
  • PAC-1 抗体 4O21 是小鼠单克隆 IgG2a κ，PAC-1抗体, 在14篇文献中引用，规格为200 µg/ml
  • 针对human来源的重组PAC-1
  • PAC-1 抗体 (4O21) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 PAC-1
  • 抗PAC-1抗体（4O21）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PAC-1 (4O21): sc-32776的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 PAC-1 抗体 (4O21) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与PAC-1 抗体 (4O21) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

PAC-1抗体（4O21）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测人类源性PAC-1蛋白。PAC-1 (4O21) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物，可满足不同实验需求。PAC-1蛋白在调节细胞分裂原活化蛋白（MAP）激酶中起着至关重要的作用，而MAP激酶对于调解细胞对各种刺激（包括生长因子和应激信号）的反应至关重要。通过选择性脱磷酸化MAP激酶中的关键磷硫氨酸和磷酪氨酸残基，PAC-1有助于维持细胞内环境平衡，防止可能导致病理状况的异常信号。PAC-1的表达受多种生长因子诱导，这凸显了PAC-1在细胞信号传导通路中控制细胞增殖、分化及存活的重要性。人类PAC-1位于2q11染色体上，编码一种314个氨基酸的蛋白质，这使得PAC-1在复杂的细胞信号传导网络中扮演着重要角色，也是癌症和其他MAP激酶信号传导失调疾病研究的重要靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PAC-1 抗体 (4O21) 参考文献:

  1. 双特异性磷酸酶：控制 MAP 激酶功能的基因家族。  |  Camps, M., et al. 2000. FASEB J. 14: 6-16. PMID: 10627275
  2. 大颗粒淋巴细胞白血病中 PAC-1 变异的特征。  |  Kothapalli, R., et al. 2003. Protein Expr Purif. 32: 52-60. PMID: 14680939
  3. PAC-1 双特异性磷酸酶可预测浆液性卵巢癌的不良预后。  |  Givant-Horwitz, V., et al. 2004. Gynecol Oncol. 93: 517-23. PMID: 15099972
  4. Procaspase-Activating Compound 1 (PAC-1) 的衍生物及其抗癌活性。  |  Roth, HS. and Hergenrother, PJ. 2016. Curr Med Chem. 23: 201-41. PMID: 26630918
  5. PAC-1 及其衍生物 WF-210 通过抑制血管内皮生长因子/血管内皮生长因子受体途径抑制血管生成。  |  Wang, F., et al. 2018. Eur J Pharmacol. 821: 29-38. PMID: 29269017
  6. 急性缺血性中风中 PAC-1、CD62P 和血小板-白细胞聚集的临床意义。  |  Zhang, Y., et al. 2022. Bull Exp Biol Med. 172: 543-548. PMID: 35348954
  7. 治疗晚期恶性肿瘤的促天冬酶激活化合物-1（PAC-1）I 期研究。  |  Danciu, OC., et al. 2023. Br J Cancer. 128: 783-792. PMID: 36470974
  8. 联合使用 PAC-1 和 entrectinib 治疗转移性葡萄膜黑色素瘤。  |  Boudreau, MW., et al. 2023. Melanoma Res. 33: 514-524. PMID: 37738028
  9. 新出现的双特异性 MAP 激酶磷酸化酶家族。  |  Keyse, SM. 1995. Biochim Biophys Acta. 1265: 152-60. PMID: 7696343
  10. 丝裂原活化蛋白激酶途径的激活诱导了 PAC-1 磷酸酶基因的转录。  |  Grumont, RJ., et al. 1996. Mol Cell Biol. 16: 2913-21. PMID: 8649402
  11. 新型丝裂原活化蛋白激酶磷酸酶 MKP-4 的分子克隆和功能表征。  |  Muda, M., et al. 1997. J Biol Chem. 272: 5141-51. PMID: 9030581
  12. 双特异性磷酸酶的功能研究。  |  Sun, H. 1998. Methods Mol Biol. 84: 307-18. PMID: 9666459

订购信息

产品名称产品编号规格价格数量收藏夹

PAC-1 抗体 (4O21)

sc-32776
200 µg/ml
RMB2377.00

PAC-1 (4O21): m-IgG Fc BP-HRP 套装

sc-525538
200 µg Ab; 10 µg BP
RMB2662.00

PAC-1 抗体 (4O21) AC

sc-32776 AC
500 µg/ml, 25% agarose
RMB3129.00

PAC-1 抗体 (4O21) HRP

sc-32776 HRP
200 µg/ml
RMB2377.00

PAC-1 抗体 (4O21) FITC

sc-32776 FITC
200 µg/ml
RMB2482.00

PAC-1 抗体 (4O21) PE

sc-32776 PE
200 µg/ml
RMB2580.00

PAC-1 抗体 (4O21) Alexa Fluor® 488

sc-32776 AF488
200 µg/ml
RMB2685.00

PAC-1 抗体 (4O21) Alexa Fluor® 546

sc-32776 AF546
200 µg/ml
RMB2685.00

PAC-1 抗体 (4O21) Alexa Fluor® 594

sc-32776 AF594
200 µg/ml
RMB2685.00

PAC-1 抗体 (4O21) Alexa Fluor® 647

sc-32776 AF647
200 µg/ml
RMB2685.00

PAC-1 抗体 (4O21) Alexa Fluor® 680

sc-32776 AF680
200 µg/ml
RMB2685.00

PAC-1 抗体 (4O21) Alexa Fluor® 790

sc-32776 AF790
200 µg/ml
RMB2685.00

Can this antibody be used against human platelets?

Asked by: Ksuke
Thank you for your question. Yes, PAC-1 Antibody (4O21) is recommended for detection of PAC-1 of human origin by WB, IP, IF and IHC(P).
Answered by: Jiajun Z
Date published: 2024-08-05

PAC-1 抗体 (4O21) PE（sc-32776 PE）はFCMにも使える蛍光標識抗体でしょうか？

Asked by: armic
質問あり、がとうございます。sc-32776 PE はFCM、IFにも使える蛍光標識抗体です。宜しくお願い致します。 Thank you for your question. sc-32776 PE is a fluorescently conjugated antibody that can be used for FCM and IF. Thank you.
Answered by: Jiajun Z
Date published: 2024-03-29

Can PAC-1 (4O21): sc-32776 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PAC-1 (4O21): sc-32776 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2018-04-04
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Rated 3 out of 5 by from No visible band in human airway smooth muscleNo band in western from 15ug total protein using human airway smooth muscle cell lysates (1:200).
Date published: 2017-07-31
Rated 5 out of 5 by from used for WBp-akt1 antibody for WB use, I suggest diluted this antibody at 1:500.
Date published: 2017-02-24
Rated 5 out of 5 by from Excellent AntibodyThe PAC-1 Antibody (4O21): sc-32776 works very well for multiple applications including WB, IP, IF and IHC(P). This product has also been cited several times for further verification.
Date published: 2017-01-20
Rated 5 out of 5 by from StrongStrong, clean band in western blot analysis of PAC-1 expression in HeLa whole cell lysate. -SCBT QC
Date published: 2015-07-06
Rated 5 out of 5 by from Used for western blotting with human ectopicUsed for western blotting with human ectopic stromal cells. -SCBT Publication Review
Date published: 2015-06-22
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human lateral ventricle tissue. -SCBT QC
Date published: 2015-05-19
Rated 5 out of 5 by from Positive experiencePositive experience
Date published: 2015-04-09
Rated 5 out of 5 by from Published western blot data using human MB435Published western blot data using human MB435 cell lysates. -SCBT Publication Review
Date published: 2015-02-06
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PAC-1 抗体 (4O21) is rated 4.8 out of 5 by 8.
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