Date published: 2025-9-6
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p53 Antibody (Pab 240) - Western Blotting - Image 294675
未经处理 (A, D), CHK 1 处理 (B, E) 和 DNA-PK 处理 (C, F) 的 p53 重组蛋白中 p53 磷酸化的 Western 印迹分析. 测试的抗体包括 p-p53 (Thr 18) -R: sc-16716-R (A, B, C) 和 p53 (Pab 240): sc-99 (D, E, F).
未经处理 (A, D), CHK 1 处理 (B, E) 和 DNA-PK 处理 (C, F) 的 p53 重组蛋白中 p53 磷酸化的 Western 印迹分析. 测试的抗体包括 p-p53 (Thr 18) -R: sc-16716-R (A, B, C) 和 p53 (Pab 240): sc-99 (D, E, F).

p53 抗体 (Pab 240): sc-99

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寫評論提問

说明书
  • p53抗体(Pab 240)是小鼠单克隆IgG1 κ, 在474篇文献中引用，规格为200 µg/ml
  • 免疫human物种的p53的氨基酸213-217
  • p53 抗体 (Pab 240) 推荐用于WB, IP 和 IF ，检测 mouse, rat, human 和 来源的only mutant p53 under non-denaturing conditions but is equally reactive with mutant and wildtype p53 under denaturing conditions; 也和以下物种反应，包括： bovine and avian
  • 抗p53抗体（Pab 240）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p53 (Pab 240): sc-99的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是p53 Antibody (Pab 240) for WB applications. 的首选辅助检测试剂，这些试剂现与p53 Antibody (Pab 240) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p53抗体(Pab 240)是一种小鼠IgG1 κ轻链单克隆抗体,可识别位于人类p53蛋白质氨基酸213-217之间的表位。p53单克隆抗体(Pab 240)以能在非变性条件下检测p53突变形式的能力而闻名,在变性条件下,p53单克隆抗体(Pab 240)可与人类、小鼠和大鼠的突变型和野生型p53发生反应。抗p53抗体(Pab 240)可用于包括蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）在内的多种应用,并具有多种偶联形式,如非偶联、琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联形式。p53蛋白是一种重要的肿瘤抑制因子，作为DNA结合转录因子发挥作用，在细胞应激时影响细胞周期阻滞、凋亡和DNA修复机制。p53主要定位于细胞核，可受MDM2的调节，MDM2是E3泛素连接酶，可促进p53出核并靶向其进行蛋白酶体降解。p53的DNA结合域(氨基酸110-286)发生突变,包括影响p53 IgG1 κ单克隆抗体(Pab 240)识别的表位的突变，可损害其dna结合能力，并通常与多种人类癌症相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p53 抗体 (Pab 240) 参考文献:

  1. p53电路板。  |  Sullivan, KD., et al. 2012. Biochim Biophys Acta. 1825: 229-44. PMID: 22333261
  2. 在蛋白质水平上检测p53同工型。  |  Marcel, V., et al. 2013. Methods Mol Biol. 962: 15-29. PMID: 23150434
  3. 紫外线照射后啮齿动物角膜上皮细胞培养物中p53蛋白亚细胞定域和细胞凋亡。  |  Tendler, Y., et al. 2013. Int J Mol Med. 31: 540-6. PMID: 23338225
  4. PHD3通过羟基化作用调节p53蛋白质的稳定性。  |  Rodriguez, J., et al. 2018. Cell Rep. 24: 1316-1329. PMID: 30067985
  5. 通过工程蛋白晶体在细胞内高效传递 p53 蛋白，恢复体内抑制肿瘤的功能。  |  Yang, Z., et al. 2021. Biomaterials. 271: 120759. PMID: 33798968
  6. 以P53蛋白质为靶点进行癌症治疗：P53研究的转化影响。  |  Levine, AJ. 2022. Cancer Res. 82: 362-364. PMID: 35110395
  7. p53蛋白质表达和TP53变异状态在大肠癌中的临床意义。  |  Kim, KM., et al. 2022. BMC Cancer. 22: 940. PMID: 36045334
  8. 阐明p53蛋白质中各种动力学反应的含义。  |  Charan, K., et al. 2023. Chemphyschem. 24: e202200537. PMID: 36208026
  9. 靶向p53的小分子校正剂和稳定剂。  |  Fallatah, MMJ., et al. 2023. Trends Pharmacol Sci. 44: 274-289. PMID: 36964053
  10. 翻译后调节p53肿瘤抑制蛋白的功能。  |  Maxwell, SA. and Roth, JA. 1994. Crit Rev Oncog. 5: 23-57. PMID: 7948107
  11. p53蛋白的结构与基因进化：再探。  |  Soussi, T. and May, P. 1996. J Mol Biol. 260: 623-37. PMID: 8709143
  12. 功能丧失与p53蛋白质的稳定性。  |  Blagosklonny, MV. 1997. Oncogene. 15: 1889-93. PMID: 9365234

订购信息

产品名称产品编号规格价格数量收藏夹

p53 抗体 (Pab 240)

sc-99
200 µg/ml
RMB2377.00

p53 (Pab 240): m-IgG Fc BP-HRP 套装

sc-526447
200 µg Ab; 10 µg BP
RMB2662.00

p53 (Pab 240): m-IgG1 BP-HRP 套装

sc-531820
200 µg Ab; 20 µg BP
RMB2662.00

p53 抗体 (Pab 240) AC

sc-99 AC
500 µg/ml, 25% agarose
RMB3129.00

p53 抗体 (Pab 240) HRP

sc-99 HRP
200 µg/ml
RMB2377.00

p53 抗体 (Pab 240) FITC

sc-99 FITC
200 µg/ml
RMB2482.00

p53 抗体 (Pab 240) PE

sc-99 PE
200 µg/ml
RMB2580.00

p53 抗体 (Pab 240) Alexa Fluor® 488

sc-99 AF488
200 µg/ml
RMB2685.00

p53 抗体 (Pab 240) Alexa Fluor® 546

sc-99 AF546
200 µg/ml
RMB2685.00

p53 抗体 (Pab 240) Alexa Fluor® 594

sc-99 AF594
200 µg/ml
RMB2685.00

p53 抗体 (Pab 240) Alexa Fluor® 647

sc-99 AF647
200 µg/ml
RMB2685.00

p53 抗体 (Pab 240) Alexa Fluor® 680

sc-99 AF680
200 µg/ml
RMB2685.00

p53 抗体 (Pab 240) Alexa Fluor® 790

sc-99 AF790
200 µg/ml
RMB2685.00

I was wondering if I can receive the 10 µg FREE sample from p53 (Pab 240): sc-99. Thanks

Asked by: Anonymous
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-12-18

Hello, I am interested in p53 antibody (Pab 240): sc-99, but I was wondering how many ul the batch of this antibody is. Thanks, Lolita

Asked by: Anonymous
Thank you for your question. p53 antibody (Pab 240): sc-99 is provided as 200µg in 1ml (1000µl).
Answered by: Tech Service
Date published: 2019-12-18

I am trying to do immunoprecipitation using this antibody. but I am not getting any pull however the p53 Do1 antibody gives a good band. I use 1ug of antibody and I am using p53 recombinant protein 1ug. I have tried PBS and NP-40 buffer with 1% NP-40.

Asked by: Aakansha
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

I am looking to perform immunofluorescence studies in mice tissue samples. Is the p53 Antibody (Pab 240): sc-99 a viable option for this?

Asked by: Trav11
If looking to perform immunofluorescence in mice tissue samples, we recommend using our directly conjugated mouse monoclonal primary antibodies. The p53 Antibody (Pab 240): sc-99 is NOT currently available with direct conjugation, but we do offer an alternative antibody, p53 Antibody (DO-1): sc-126. This alternative (sc-126) is currently available conjugated to HRP (sc-126 HRP), phycoerythrin (sc-126 PE), fluorescein (sc-126 FITC), Alexa Fluor® 488 (sc-126 AF488), Alexa Fluor® 647 (sc-126 AF647), biotin (sc-126 B), TRITC (sc-126 TRITC, 200 µg/ml), PerCP (sc-126 PerCP), PerCP-Cy5.5 (sc-126 PCPC5), or Alexa Fluor® 405 (sc-126 AF405).
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 4 out of 5 by from A little faint, but does workI used this with human lysates for western blot. Band was a little faint, but it definitely does work. I've heard lysate prep for p53 can be tricky, so I may need to just improve my lysis buffer recipe/protocol.
Date published: 2019-01-18
Rated 1 out of 5 by from Not as expectedNot good for detection of Human p53 by WB. not tested for other applications
Date published: 2018-06-08
Rated 1 out of 5 by from no effectI received a free sample of the antibody. Unfortunately, I did not obtain any bands in both nuclear and whole cell lysates. I tried to detect human and mouse p-p53.
Date published: 2017-12-15
Rated 5 out of 5 by from Great for Western blot!We used this antibody at a 1:1000 dilution to detect the DNA-binding domain of recombinant p53 from E.coli expression. The antibody works great and there are clear, distinct bands on the gel. Would definitely recommend!
Date published: 2017-10-31
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Good for WB1:500 diluted for wb of rat protein ,the band is strong ,but the position is about 45KD
Date published: 2017-08-28
Rated 5 out of 5 by from strong band at 53kdaThis antibody been used to detect the p53 protein in mouse lung tissue.I have optimizing antibody concentrations from 1:300 to 1:1000,I prefer to use 1:500 ,this dilution got clean band at 53kda and no background.If the background is too high, I recommend use 5% BSA for blocking to reducing background.
Date published: 2017-07-01
Rated 5 out of 5 by from powerfulI tested this antibody with the lysates of WR19L cells, It is good for western blot
Date published: 2017-06-01
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p53 抗体 (Pab 240) is rated 4.3 out of 5 by 21.
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