Date published: 2025-9-6
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p53 Antibody (Pab 1801) - Immunohistochemistry - Image 160137
p53 抗体 (Pab 1801): sc-98. 福尔马林固定, 石蜡包埋的人乳腺癌组织的免疫过氧化物酶染色 (A) 和甲醇固定的 A-431 细胞的免疫荧光染色 (B), 显示核染色.
p53 抗体 (Pab 1801): sc-98. 福尔马林固定, 石蜡包埋的人乳腺癌组织的免疫过氧化物酶染色 (A) 和甲醇固定的 A-431 细胞的免疫荧光染色 (B), 显示核染色.

p53 抗体 (Pab 1801): sc-98

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寫評論提問

说明书
  • p53抗体(Pab 1801)是小鼠单克隆IgG1 κ, 在412篇文献中引用，规格为200 µg/ml
  • N-terminal表位图谱位于human起源的p53的氨基酸32-79之间
  • 抗-p53 抗体 (Pab 1801) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 p53
  • p53抗体（Pab 1801）可与琼脂糖结合用于IP，与藻红蛋白结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-98 X, 200 µg/0.1 ml)
  • 关于如何获取p53 (Pab 1801): sc-98的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 p53 抗体 (Pab 1801) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p53 抗体 (Pab 1801) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p53抗体(Pab 1801)是一种小鼠IgG1 κ轻链单克隆抗体,可通过多种应用特异性检测人、小鼠和大鼠样本中的p53肿瘤抑制蛋白,包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHC-P)和流式细胞术(FCM)。 p53小鼠单克隆抗体(Pab 1801)可识别p53蛋白质N端区域(氨基酸32-79)内的表位,可用于研究p53的表达和功能。抗p53抗体(Pab 1801)有偶联和非偶联两种形式,可满足不同的实验需求。p53是一种重要的转录因子,参与调节细胞周期停滞、细胞凋亡和DNA修复以应对细胞应激,从而发挥重要的肿瘤抑制作用 。p53通过激活调节这些过程的靶基因来发挥作用,其活性受到严格调控。p53主要定位于细胞核,但可通过负调控因子MDM2进入细胞质,MDM2是一种E3泛素连接酶,可靶向p53,使其发生蛋白酶体降解作用。p53 DNA 结合域的突变,尤其是氨基酸 110-286 内的突变,会破坏其结合 DNA 的能力,通常与人类各种癌症有关。p53 IgG1 κ单克隆抗体(Pab 1801)有助于检测野生型和特定突变型p53，使其成为多种癌症研究应用的重要试剂。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p53 抗体 (Pab 1801) 参考文献:

  1. 正常细胞和肿瘤中的P53调节和功能。  |  Liu, Y. and Kulesz-Martin, M. 2000. Medicina (B Aires). 60 Suppl 2: 9-11. PMID: 11188940
  2. p53 蛋白调节阿米福星对细胞凋亡, 细胞周期进展和细胞保护的影响。  |  Lee, EJ., et al. 2003. Br J Cancer. 88: 754-9. PMID: 12618886
  3. DNA-蛋白质交联和p53蛋白质表达与职业接触甲醛的关系。  |  Shaham, J., et al. 2003. Occup Environ Med. 60: 403-9. PMID: 12771391
  4. [p53蛋白质及其突变体在癌症治疗新策略中的相关性]。  |  Müller, P., et al. 2004. Cas Lek Cesk. 143: 313-7. PMID: 15305766
  5. 对p53的调节——对复杂过程的洞察。  |  Boehme, KA. and Blattner, C. 2009. Crit Rev Biochem Mol Biol. 44: 367-92. PMID: 19929178
  6. p53及其突变体在肿瘤细胞迁移和侵袭中的作用。  |  Muller, PA., et al. 2011. J Cell Biol. 192: 209-18. PMID: 21263025
  7. 野生型p53蛋白质作为突变型p53蛋白质的分子伴侣可导致肿瘤缺氧消退。  |  Gogna, R., et al. 2012. J Biol Chem. 287: 2907-14. PMID: 22147694
  8. p53蛋白质的同种型：病原体感染第一线的关键调节剂？  |  Terrier, O., et al. 2013. PLoS Pathog. 9: e1003246. PMID: 23592981
  9. 案例研究：MDM2-p53蛋白质-蛋白质相互作用抑制剂的发现。  |  Liu, L., et al. 2015. Methods Mol Biol. 1278: 567-85. PMID: 25859977
  10. 生物无机化学在癌症中突变p53的重新激活中的作用。  |  Miller, JJ., et al. 2022. J Biol Inorg Chem. 27: 393-403. PMID: 35488931
  11. 通过阻断野生型p53的各种抗原表位，可以调节其DNA结合活性。  |  Wolkowicz, R., et al. 1995. Oncogene. 10: 1167-74. PMID: 7535417
  12. [p53]。  |  Takahashi, R. 1997. Gan To Kagaku Ryoho. 24: 1381-5. PMID: 9309129

订购信息

产品名称产品编号规格价格数量收藏夹

p53 抗体 (Pab 1801)

sc-98
200 µg/ml
RMB2377.00

p53 (Pab 1801): m-IgG Fc BP-HRP 套装

sc-526446
200 µg Ab; 10 µg BP
RMB2662.00

p53 (Pab 1801): m-IgG1 BP-HRP 套装

sc-531819
200 µg Ab; 20 µg BP
RMB2662.00

p53 抗体 (Pab 1801) X

sc-98 X
200 µg/0.1 ml
RMB2377.00

p53 抗体 (Pab 1801) AC

sc-98 AC
500 µg/ml, 25% agarose
RMB3129.00

p53 抗体 (Pab 1801) PE

sc-98 PE
200 µg/ml
RMB2580.00

p53 抗体 (Pab 1801) Alexa Fluor® 488

sc-98 AF488
200 µg/ml
RMB2685.00

p53 抗体 (Pab 1801) Alexa Fluor® 546

sc-98 AF546
200 µg/ml
RMB2685.00

p53 抗体 (Pab 1801) Alexa Fluor® 594

sc-98 AF594
200 µg/ml
RMB2685.00

p53 抗体 (Pab 1801) Alexa Fluor® 647

sc-98 AF647
200 µg/ml
RMB2685.00

p53 抗体 (Pab 1801) Alexa Fluor® 680

sc-98 AF680
200 µg/ml
RMB2685.00

p53 抗体 (Pab 1801) Alexa Fluor® 790

sc-98 AF790
200 µg/ml
RMB2685.00

p53 抗体 (Pab 1801) Alexa Fluor® 405

sc-98 AF405
200 µg/ml
RMB2685.00

What are the differences of Anti-P53 Pab 1801,240and DO-1?If it possible to be used for mitochondria protein WB and staining?Which one is feasible?

Asked by: fwang
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-98 PE), Alexa Fluor® 488 (sc-98 AF488) or Alexa Fluor® 647 (sc-98 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2023-10-12
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Rated 4 out of 5 by from I recommend this antibodyIt was used in WB, SH-S5SY cells, and the dillution was 1/500. I totally recommend it
Date published: 2019-04-01
Rated 5 out of 5 by from Great productI used it for immunihistochemistry and working great. I will recommend this antibody to others too.
Date published: 2018-03-22
Rated 1 out of 5 by from IHC on mouse tissue not workingI tried this antibody on immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse breast carcinoma tissue at 1/50 and it did not work. No staining.
Date published: 2017-11-30
Rated 4 out of 5 by from Worked well on primary culturesI received a sample of this p53 antibody because I was having difficulty detecting p53 in my primary murine astrocyte cultures by western blot. This gave a single, clear band, although I had to use a higher titer of antibody (1:100 dilution) to detect.
Date published: 2017-10-17
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Excellent!1:250 diluted for wb of rat protein ,the band is strong with at the position of about 60KD.
Date published: 2017-08-08
Rated 5 out of 5 by from exprected resultIt works very well for IF analysis of PFA fixed cells at a dilution of 1:50,the staining is our exprected result.
Date published: 2017-07-29
Rated 5 out of 5 by from good IHC resultUses for formalin fixed tissue; IHC; routinely used over years, satisfied with results obtained.
Date published: 2017-05-27
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p53 抗体 (Pab 1801) is rated 4.6 out of 5 by 18.
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