Date published: 2025-9-6
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p53 Antibody (DO-7) - Western Blotting - Image 349922
p53 抗体 (DO-7): sc-47698. A-431 (A), HCT-116 (B), Jurkat (C), SW480 (D), BT-20 (E) 和 HUV-EC-C (F) 全细胞裂解液中 p53 表达的 Western 印迹分析.
p53 抗体 (DO-7): sc-47698. A-431 (A), HCT-116 (B), Jurkat (C), SW480 (D), BT-20 (E) 和 HUV-EC-C (F) 全细胞裂解液中 p53 表达的 Western 印迹分析.

p53 抗体 (DO-7): sc-47698

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寫評論提問

说明书
  • p53抗体(DO-7)是小鼠单克隆IgG2b κ, 在255篇文献中引用，规格为200 µg/ml
  • human p53 1-45的氨基酸之间的表位图谱
  • p53 抗体 (DO-7) 推荐用于WB, IP, IF, IHC(P) 和 FCM，检测human 来源的 both wildtype and mutant p53 under denaturing and non-denaturing conditions; 不会与 p53 of mouse or rat origin产生交联反应
  • 抗p53抗体（DO-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p53 (DO-7): sc-47698的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 p53 抗体 (DO-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p53 抗体 (DO-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p53抗体(DO-7)是一种IgG2b κ链的小鼠单克隆抗体，能特异性靶向人p53蛋白，识别位于人类p53第1-45氨基酸内的表位。p53小鼠单克隆抗体(DO-7)在变性及非变性条件下均可敏感、特异性地检测野生型和突变型p53。抗p53抗体(DO-7)广泛用于研究应用,特别是对福尔马林固定、石蜡包埋(FFPE)组织的免疫组织化学研究,可有效检测各种人类肿瘤中p53的过度表达。p53单克隆抗体(DO-7)可用于免疫印迹、免疫沉淀、免疫荧光、免疫组织化学和流式细胞术。p53蛋白质(也称为TP53)是一种重要的肿瘤抑制蛋白,参与调节细胞周期停滞、细胞凋亡、衰老、DNA修复和细胞应激的代谢反应。它作为转录因子发挥作用，激活参与这些过程的基因,从而促进上述过程。p53的活性受到MDM2的严格调控,MDM2是一种E3泛素连接酶，可将p53泛素化，导致其蛋白酶体降解。p53 DNA 结合结构域(氨基酸 110-286)的突变常见于人类癌症,会削弱其肿瘤抑制功能。p53 抗体(DO-7)有非偶联形式和多种偶联形式,包括琼脂糖、HRP、PE、FITC 和各种 Alexa Fluor® 偶联形式,可灵活应用于多种应用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p53 抗体 (DO-7) 参考文献:

  1. p53依赖性细胞周期控制：对遗传毒性应激的反应。  |  Schwartz, D. and Rotter, V. 1998. Semin Cancer Biol. 8: 325-36. PMID: 10101798
  2. 台湾乙烯基氯化物单体工人血浆p53蛋白质和抗p53抗体表达。  |  Luo, JC., et al. 1999. J Occup Environ Med. 41: 521-6. PMID: 10390705
  3. p300/CBP/p53相互作用与p53反应的调节。  |  Grossman, SR. 2001. Eur J Biochem. 268: 2773-8. PMID: 11358491
  4. p53通路：正反馈环和负反馈环。  |  Harris, SL. and Levine, AJ. 2005. Oncogene. 24: 2899-908. PMID: 15838523
  5. p53：生殖领域的新角色。  |  Hu, W., et al. 2008. Cell Cycle. 7: 848-52. PMID: 18414047
  6. 细胞对缺氧的适应和p53转录调节。  |  Zhao, Y., et al. 2009. J Zhejiang Univ Sci B. 10: 404-10. PMID: 19434769
  7. 癌症中靶向p53的当前策略。  |  Chen, F., et al. 2010. Biochem Pharmacol. 80: 724-30. PMID: 20450892
  8. 肿瘤抑制蛋白p53：从结构到药物发现。  |  Joerger, AC. and Fersht, AR. 2010. Cold Spring Harb Perspect Biol. 2: a000919. PMID: 20516128
  9. 长非编码 RNA PANDA 有助于 p53 抑癌基因的稳定  |  Kotake, Y., et al. 2016. Anticancer Res. 36: 1605-11. PMID: 27069137
  10. 野生型和癌症相关突变型p53的转录调控。  |  Pfister, NT. and Prives, C. 2017. Cold Spring Harb Perspect Med. 7: PMID: 27836911

订购信息

产品名称产品编号规格价格数量收藏夹

p53 抗体 (DO-7)

sc-47698
200 µg/ml
RMB2377.00

p53 (DO-7): m-IgG Fc BP-HRP 套装

sc-525553
200 µg Ab; 10 µg BP
RMB2662.00

p53 (DO-7): m-IgG2b BP-HRP 套装

sc-549580
200 µg Ab; 10 µg BP
RMB2662.00

p53 抗体 (DO-7) AC

sc-47698 AC
500 µg/ml, 25% agarose
RMB3129.00

p53 抗体 (DO-7) HRP

sc-47698 HRP
200 µg/ml
RMB2377.00

p53 抗体 (DO-7) FITC

sc-47698 FITC
200 µg/ml
RMB2482.00

p53 抗体 (DO-7) PE

sc-47698 PE
200 µg/ml
RMB2580.00

p53 抗体 (DO-7) Alexa Fluor® 488

sc-47698 AF488
200 µg/ml
RMB2685.00

p53 抗体 (DO-7) Alexa Fluor® 546

sc-47698 AF546
200 µg/ml
RMB2685.00

p53 抗体 (DO-7) Alexa Fluor® 594

sc-47698 AF594
200 µg/ml
RMB2685.00

p53 抗体 (DO-7) Alexa Fluor® 647

sc-47698 AF647
200 µg/ml
RMB2685.00

p53 抗体 (DO-7) Alexa Fluor® 680

sc-47698 AF680
200 µg/ml
RMB2685.00

p53 抗体 (DO-7) Alexa Fluor® 790

sc-47698 AF790
200 µg/ml
RMB2685.00

What is the best secondary to use with this mouse antibody?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 1
Date published: 2017-01-13

Is it possible to use this antibody for IF or IHC on mouse tissue samples?

Asked by: Anna99
Yes, this antibody is recommended for detection in mouse. It is available conjugated to either phycoerythrin (sc-47698 PE) or fluoroscein (sc-47698 FITC), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Service 11
Date published: 2021-12-13
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Rated 4 out of 5 by from Good antibody!SC-47698 suitable for WB from total extract of keratinocytes cell line, very good results.
Date published: 2024-12-10
Rated 5 out of 5 by from Great staining on equine sarcoidsWe tested this on equine sarcoid and staining was good using HIER with s EDTA pH9 buffer and a antibody dilution of 1:50.
Date published: 2023-04-21
Rated 5 out of 5 by from Worked perfectThis antibody worked great, I incubated overnight and the signal was strong.
Date published: 2019-07-14
Rated 5 out of 5 by from Excellent antibody for WB detection of p53Excellent antibody for WB detection of p53 in human cell extracts. Clean band!
Date published: 2019-02-21
Rated 5 out of 5 by from P53条带特别好做出来这是最好做的抗体，无论多低的浓度都可以做出来，特别实用！感觉做实验要是所有的抗体都是这么好出条带就好了，特别好做，点赞！
Date published: 2017-12-07
Rated 5 out of 5 by from Good AntibodyWe see clear contrast between cisplatin-treated and non-treated.
Date published: 2017-11-20
Rated 5 out of 5 by from Antibody for WBP53 antibody was used in HCC cell lines, providing clear and specific results through WB.
Date published: 2017-10-31
Rated 3 out of 5 by from Not very goodWe changed the experiment conditions about the dilution and incubated time, however, always have someweak non-specific bands.I don't know what's wrong? However, it has strong target band.But the membrance have non-specific bands not suitable for paper publishing, I am still improving the conditions.
Date published: 2017-09-10
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p53 抗体 (DO-7) is rated 4.7 out of 5 by 30.
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