Date published: 2025-9-6
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p53 Antibody (DO-2) - Western Blotting - Image 390075
p53 抗体 (DO-2) HRP: sc-53394 HRP. A-431 (A), BT-20 (B), MDA-MB-231 (C) 和 T-47D (D) 全细胞裂解液中 p53 表达的直接 Western 印迹分析.
p53 抗体 (DO-2) HRP: sc-53394 HRP. A-431 (A), BT-20 (B), MDA-MB-231 (C) 和 T-47D (D) 全细胞裂解液中 p53 表达的直接 Western 印迹分析.

p53 抗体 (DO-2): sc-53394

4.6(5)
寫評論提問

说明书
  • p53抗体(DO-2)是小鼠单克隆IgG1 κ, 在32篇文献中引用，规格为200 µg/ml
  • 免疫human物种的p53的氨基酸10-16
  • 抗-p53 抗体 (DO-2) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 p53
  • 抗p53抗体（DO-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p53 (DO-2): sc-53394的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 p53 抗体 (DO-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p53 抗体 (DO-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p53抗体(DO-2)是一种小鼠IgG1 κ轻链单克隆抗体,特异性靶向小鼠、大鼠和人源的p53蛋白质(也称为TP53)。p53单克隆抗体(DO-2)识别位于p53蛋白质第10-16个氨基酸之间的表位,广泛用于多种应用,包括蛋白质印迹、免疫沉淀、免疫荧光和石蜡包埋组织的免疫组织化学。p53单克隆抗体(DO-2)有非偶联和多种偶联形式,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 偶联物,是检测肿瘤抑制因子 p53 的理想抗体,p53 是细胞周期控制、细胞凋亡和 DNA 修复的重要调节因子。p53蛋白质通过上调与生长停滞和程序性细胞死亡相关的基因来对细胞应激信号做出反应。它主要定位于细胞核,但可通过MDM2(一种E3泛素连接酶，通过标记p53进行蛋白酶体降解来调节p53)转移到细胞质中。这种调节使p53在磷酸化和乙酰化等翻译后修饰的驱动下，在非活性和活性状态之间波动。p53 的 DNA 结合域(氨基酸 110-286)突变会削弱其DNA结合能力,从而导致各种癌症。抗 p53 抗体(DO-2)在研究 p53 在肿瘤抑制、细胞分化中的作用及其在不同生理和病理条件下的动态调节方面尤为有用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p53 抗体 (DO-2) 参考文献:

  1. p53和细胞周期控制：无处不在。  |  North, S. and Hainaut, P. 2000. Pathol Biol (Paris). 48: 255-70. PMID: 10858958
  2. P53反应基因与癌症诊断和治疗开发的潜力。  |  Xu, H. and el-Gewely, MR. 2001. Biotechnol Annu Rev. 7: 131-64. PMID: 11686042
  3. 人子宫平滑肌瘤中的p53肿瘤抑制蛋白含量及其受17β-雌二醇下调的情况。  |  Gao, Z., et al. 2002. J Clin Endocrinol Metab. 87: 3915-20. PMID: 12161533
  4. 通过染色质免疫沉淀检测p53蛋白质的转录活性。  |  Yang-Hartwich, Y., et al. 2015. Methods Mol Biol. 1219: 87-93. PMID: 25308264
  5. 环状β-发夹对MDM2/p53蛋白质相互作用具有重要的抑制作用。  |  Danelius, E., et al. 2016. Org Biomol Chem. 14: 10386-10393. PMID: 27731454
  6. p53反应元件中倒置重复序列形成的结构决定了p53蛋白质的转激活活性。  |  Brázda, V., et al. 2017. Biochem Biophys Res Commun. 483: 516-521. PMID: 28007599
  7. 评估单点突变对蛋白质热力学稳定性的影响，并分析p53蛋白质的突变图谱。  |  Banerjee, A. and Mitra, P. 2020. J Chem Inf Model. 60: 3315-3323. PMID: 32401507
  8. p53和人类TLRs相互作用的计算分子表征。  |  JahoorAlam, M., et al. 2022. Cell Mol Biol (Noisy-le-grand). 67: 1-5. PMID: 35818278
  9. 针对突变型p53-Y220C亚位点空穴的多功能金属辅助蛋白修饰。  |  Miller, JJ., et al. 2023. J Inorg Biochem. 242: 112164. PMID: 36871418
  10. 设计一种合成Mdm2结合小蛋白，在体内发挥激活p53反应的作用。  |  Böttger, A., et al. 1997. Curr Biol. 7: 860-9. PMID: 9382809

订购信息

产品名称产品编号规格价格数量收藏夹

p53 抗体 (DO-2)

sc-53394
200 µg/ml
$316.00

p53 (DO-2): m-IgG Fc BP-HRP 套装

sc-526806
200 µg Ab; 10 µg BP
$354.00

p53 (DO-2): m-IgG1 BP-HRP 套装

sc-532179
200 µg Ab; 20 µg BP
$354.00

p53 抗体 (DO-2) AC

sc-53394 AC
500 µg/ml, 25% agarose
$416.00

p53 抗体 (DO-2) HRP

sc-53394 HRP
200 µg/ml
$316.00

p53 抗体 (DO-2) FITC

sc-53394 FITC
200 µg/ml
$330.00

p53 抗体 (DO-2) PE

sc-53394 PE
200 µg/ml
$343.00

p53 抗体 (DO-2) Alexa Fluor® 488

sc-53394 AF488
200 µg/ml
$357.00

p53 抗体 (DO-2) Alexa Fluor® 546

sc-53394 AF546
200 µg/ml
$357.00

p53 抗体 (DO-2) Alexa Fluor® 594

sc-53394 AF594
200 µg/ml
$357.00

p53 抗体 (DO-2) Alexa Fluor® 647

sc-53394 AF647
200 µg/ml
$357.00

p53 抗体 (DO-2) Alexa Fluor® 680

sc-53394 AF680
200 µg/ml
$357.00

p53 抗体 (DO-2) Alexa Fluor® 790

sc-53394 AF790
200 µg/ml
$357.00

I want to know what is the difference of P53(DO-1) and (DO-2)? Which antibody can detect the mutant P53 in human breast cancer BT474?

Asked by: yangkk
感谢您的提问。如果您能直接拨打(+86) 021-60936350，或者发邮件到我们亚洲服务部的邮箱[email protected]我们就这个问题和其他相关问题进行更多的互动讨论，那将会很有帮助。 Thank you for your question.It would be helpful if you could call us directly at (+86) 021-60936350 or email our Asia Service department at [email protected] for more interactive discussions on this and other related issues.
Answered by: Zhanglong
Date published: 2021-08-31

Can I use p53 Antibody (DO-2): sc-53394 to perform immunohistochemistry/immunofluorescence on zefrafish brain and suggest the appropriate secondary antibody for the same.

Asked by: abhishekjain
Thank you for your question. We are aware of one product citation using this antibody in zebrafish samples. Please refer to PMID: 30073783 for the details. This is a mouse monoclonal IgG1, we would recommend using one of our fluorescently conjugated mouse IgG kappa binding proteins as a secondary antibody for detection. A complete list of avaialble conjugates is available below. https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Technical Support
Date published: 2019-07-13

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-53394: p53 (DO-2) mouse monoclonal antibody will work?

Asked by: Germaine
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Great features!Protein from HEK cells. Extracted with ripa. Loading 15ug/wells
Date published: 2017-12-12
Rated 5 out of 5 by from Great for WBThe p53 Antibody (DO-2): sc-53394 provides excellent data for Western blot, showing clear banding with minimal background. This antibody has also been cited multiple times for further verification.
Date published: 2017-01-26
Rated 5 out of 5 by from Worked well in WB with MCF7Worked well in WB with MCF7, JF305 and 973. -SCBT QC
Date published: 2015-01-15
Rated 4 out of 5 by from Antibody is reasonably specific and sensitive in AAntibody is reasonably specific and sensitive in A-431, MCF7, BT-20 and MDA-MB-231 whole cell lysates. -SCBT QC
Date published: 2015-01-07
Rated 4 out of 5 by from Antibody can detect both human and mouseAntibody can detect both human and mouse p53 transfected proteins. -SCBT QC
Date published: 2014-02-06
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p53 抗体 (DO-2) is rated 4.6 out of 5 by 5.
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