Date published: 2025-9-6
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p53 Antibody (DO-1) - Western Blotting - Image 385951
用 p53 抗体 (DO-1) AF680: sc-126 AF680 检测 A-431 (A) 和 SW480 (B) 全细胞裂解液中 p53 的表达, 用 GAPDH (0411) Alexa Fluor® 790: sc-47724 AF790 检测 GAPDH 的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
用 p53 抗体 (DO-1) AF680: sc-126 AF680 检测 A-431 (A) 和 SW480 (B) 全细胞裂解液中 p53 的表达, 用 GAPDH (0411) Alexa Fluor® 790: sc-47724 AF790 检测 GAPDH 的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

p53 抗体 (DO-1): sc-126

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说明书
  • p53抗体(DO-1)是小鼠单克隆IgG2a κ p53抗体, 在6,680篇文献中引用，规格为200 µg/ml
  • N-terminal表位图谱基于human来源的p53的氨基酸残基11-25
  • p53 抗体 (DO-1) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 wild type and mutant p53 under denaturing and non-denaturing conditions
  • 抗-p53抗体（DO-1）可结合琼脂糖用于IP；结合HRP用于WB、IHC(P)和ELISA；结合phycoerythrin或FITC用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联生物素 用于WB, IHC(P) 以及 ELISA;偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-126 X, 200 µg/0.1 ml)
  • 关于如何获取p53 (DO-1): sc-126的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 p53 抗体 (DO-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p53 抗体 (DO-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p53抗体(DO-1)是一种IgG2a kappa轻链单克隆抗体,专门用于检测人类、小鼠和大鼠中的p53(也称为TP53)。p53抗体(DO-1)识别p53 N-末端氨基酸11-25内的表位,因此能够检测该蛋白质的野生型以及某些突变形式。p53单克隆抗体(DO )用途广泛,可用于多种应用,如蛋白质印迹、免疫沉淀、免疫荧光、石蜡包埋切片免疫组织化学和流式细胞术。p53 (DO-1) 小鼠单克隆抗体有非偶联形式以及各种偶联形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物,可满足不同实验需求。p53蛋白质是一种重要的肿瘤抑制蛋白,通过调节细胞周期和诱导细胞凋亡来应对DNA损伤和其他细胞压力,在维持基因组完整性方面发挥着至关重要的作用。p53蛋白质由位于人类17号染色体上的TP53基因编码。p53主要定位于细胞核,但可通过负调控因子MDM2转运至细胞质。MDM2是一种E3泛素连接酶,可靶向p53蛋白,使其发生蛋白酶体降解作用。磷酸化作用和乙酰化作用等翻译后修饰可调节p53的活性,而磷酸化特异性抗p53抗体则可区分其磷酸化状态和非磷酸化状态。p53蛋白的 DNA 结合域(氨基酸 110-286)突变与多种人类癌症有关,因此 p53 抗体(DO-1)成为癌症研究以及细胞周期控制、细胞凋亡和肿瘤发生研究的重要试剂。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p53 抗体 (DO-1) 参考文献:

  1. p53 网站和人类癌症中 p53 基因突变的分析：建立流行病学与致癌之间的联系。  |  Soussi, T., et al. 2000. Hum Mutat. 15: 105-13. PMID: 10612830
  2. 调节 p53 的稳定性。  |  Ashcroft, M. and Vousden, KH. 1999. Oncogene. 18: 7637-43. PMID: 10618703
  3. 四聚化在 p53 功能中的作用。  |  Chène, P. 2001. Oncogene. 20: 2611-7. PMID: 11420672
  4. 人类肿瘤中 p53 磷酸化的不同模式。  |  Minamoto, T., et al. 2001. Oncogene. 20: 3341-7. PMID: 11423984
  5. 调节 p53 的特定 DNA 结合功能。  |  Hupp, TR., et al. 1992. Cell. 71: 875-86. PMID: 1423635
  6. 人类 p53 特异性单克隆抗体的分离及其在人类 p53 表达研究中的应用。  |  Banks, L., et al. 1986. Eur J Biochem. 159: 529-34. PMID: 2428616
  7. 靶向突变型p53蛋白，实现有效的癌症治疗。  |  Bykov, VJN., et al. 2018. Nat Rev Cancer. 18: 89-102. PMID: 29242642
  8. 小分子调节p53蛋白质-蛋白质相互作用。  |  Kuusk, A., et al. 2020. Biol Chem. 401: 921-931. PMID: 32049643
  9. 对p53蛋白质的全面研究。  |  Patil, MR. and Bihari, A. 2022. J Cell Biochem. 123: 1891-1937. PMID: 36183376
  10. 抑制p53蛋白质聚集作为癌症治疗策略。  |  Kwan, K., et al. 2023. Curr Opin Chem Biol. 72: 102230. PMID: 36436275
  11. p53蛋白在细胞凋亡反应中的作用。  |  Lane, DP., et al. 1994. Philos Trans R Soc Lond B Biol Sci. 345: 277-80. PMID: 7846126
  12. p53，细胞生长和分裂的守门员。  |  Levine, AJ. 1997. Cell. 88: 323-31. PMID: 9039259

订购信息

产品名称产品编号规格价格数量收藏夹

p53 抗体 (DO-1)

sc-126
200 µg/ml
RMB2377.00

p53 (DO-1): m-IgG Fc BP-HRP 套装

sc-528099
200 µg Ab; 10 µg BP
RMB2662.00

p53 (DO-1): m-IgGκ BP-HRP 套装

sc-520397
200 µg Ab, 40 µg BP
RMB2662.00

p53 (DO-1): m-IgG2a BP-HRP 套装

sc-547024
200 µg Ab; 10 µg BP
RMB2662.00

p53 抗体 (DO-1) X

sc-126 X
200 µg/0.1 ml
RMB2377.00

p53 抗体 (DO-1) AC

sc-126 AC
500 µg/ml, 25% agarose
RMB3129.00

p53 抗体 (DO-1) HRP

sc-126 HRP
200 µg/ml
RMB2377.00

p53 抗体 (DO-1) FITC

sc-126 FITC
200 µg/ml
RMB2482.00

p53 抗体 (DO-1) PE

sc-126 PE
200 µg/ml
RMB2580.00

p53 抗体 (DO-1) Alexa Fluor® 488

sc-126 AF488
200 µg/ml
RMB2685.00

p53 抗体 (DO-1) Alexa Fluor® 546

sc-126 AF546
200 µg/ml
RMB2685.00

p53 抗体 (DO-1) Alexa Fluor® 594

sc-126 AF594
200 µg/ml
RMB2685.00

p53 抗体 (DO-1) Alexa Fluor® 647

sc-126 AF647
200 µg/ml
RMB2685.00

p53 抗体 (DO-1) Alexa Fluor® 680

sc-126 AF680
200 µg/ml
RMB2685.00

p53 抗体 (DO-1) Alexa Fluor® 790

sc-126 AF790
200 µg/ml
RMB2685.00

p53 抗体 (DO-1) B

sc-126 B
200 µg/ml
RMB2407.00

p53 抗体 (DO-1) TRITC

sc-126 TRITC
200 µg/ml
RMB2407.00

p53 抗体 (DO-1) Alexa Fluor® 405

sc-126 AF405
200 µg/ml
RMB2685.00

Hi I recently purchased this antibody and I am using mouse T22 cells. I don't detect p53 at all. Does it cross-react with mouse ? I tried multiple times now

Asked by: Moiz
I tested the antibody in many mouse tissues for WB with secondary antibody recognizing light chain, and I did not get any blot. Besides, it works well in human sample.
Answered by: Lantern
Date published: 2025-05-23

Has anyone used this with mouse retinal pigment epithelial cells (RPE)? Have tried a few different IF protocols using this antibody and receive no signal.

Asked by: Konrad
Thank you for your question. We do not have any data or publications for mouse retinal pigment epithelial cells. To trouble shoot the antibody please contact technical service. Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-08-02

Does this Ab (sc-126) contain any glycine, Tris and glycerol? If yes, what are the concentrations of them?

Asked by: Hong
Thank you for your question. Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: BlakeJ
Date published: 2024-06-14

Is P53 DO-1 suitable to detect phosphorylated form  of P53?

Asked by: Anonymous
Thank you for your question. Based on sequence analysis we can determine that is possible for detection of the phosphorylated form although we can not guarantee.
Answered by: BlakeJ
Date published: 2024-05-02

How to dilute the sc-126 HRP antibody for WB? And for how long should the WB be incubated?

Asked by: llllllllllllll
Thank you for your question. Here is our recommended buffer. : https://www.scbt.com/p/tbs-blotto-a?requestFrom=search . Incubate the antibody overnight at 4 degrees. Here is our protocol : https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2024-03-28

ウエスタンブロットの実験に使用するにあたり、ネガティブコントロールとなる細胞株などはありますでしょうか？

Asked by: dm21030
質問頂き、誠にありがとうございます。下のウェブサイトをご参考ください。宜しくお願い致します。 https://www.proteinatlas.org/ENSG00000141510-TP53/cell+line Thank you very much for your question. Please refer to the website below. Thank you. https://www.proteinatlas.org/ENSG00000141510-TP53/cell Line
Answered by: Jiajun Z
Date published: 2023-09-05

What's the reactivity of the antibody? It can be used in mouse cell lines by WB? 

Asked by: fn89
Thank you for your question. p53 Antibody (DO-1) is recommended for detection of wild type and mutant p53 under denaturing and non-denaturing conditions of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM and can be used in western blotting experiments using murine cell lines.
Answered by: Tech Support Europe
Date published: 2023-08-28

Do you have an antibody that only detects the wild type p53 and not the mutant p53?

Asked by: SH_mountsinai
Thank you for your question . We do not have a p53 antibody that only detects the wild type.
Answered by: BlakeJ
Date published: 2023-07-12
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Rated 1 out of 5 by from nonspecificnonspecific binding, no signal around 5. Tons around 100 and 200
Date published: 2025-07-31
Rated 4 out of 5 by from Very good!The DO-1 antibody demonstrates high specificity and sensitivity for detecting the human p53 from keratinocytes. It is particularly suitable for Western blot (WB) detect p53.
Date published: 2024-12-10
Rated 4 out of 5 by from Works for WBWorks well on neonatal mouse lungs at concentration of 30ug. Ab dilution will be 1:200 for overnight @ 4oC. It works only on a nuclear fraction
Date published: 2024-12-10
Rated 5 out of 5 by from ExcellentWorks very well. Clean blots and not much background.
Date published: 2024-09-24
Rated 5 out of 5 by from Awesome antibodyThe antibody is quite good. It is very classical and well-established antibody. The signal is strong and the specificity is very high. High recommended!
Date published: 2024-05-13
Rated 4 out of 5 by from 使いやすいサンプルを頂き、とても使いやすかったので購入を検討しています。ノンスペもなくバンドをはっきり検出できます。
Date published: 2024-04-03
Rated 5 out of 5 by from well function in WB and IFGood results when tested p53 in western blot of whole cell extracts or IFfrom rat articular chondrocytes.
Date published: 2023-12-22
Rated 5 out of 5 by from p53 antibodyThe antibody is very good. The band is clear on western blot.
Date published: 2023-08-14
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p53 抗体 (DO-1) is rated 4.6 out of 5 by 184.
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