Date published: 2025-9-11
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p38 alpha MAPK14 Antibody (F-9) - Western Blotting - Image 349989
p38α MAPK14 (F-9): sc-271120. K-562 (A), MCF7 (B), KNRK (C), A-431 (D), RAW 309 Cr. 1 (E) 和 NIH/3T3 (F) 全细胞裂解液中 p38α MAPK14 表达的 Western 印迹分析.
p38α MAPK14 (F-9): sc-271120. K-562 (A), MCF7 (B), KNRK (C), A-431 (D), RAW 309 Cr. 1 (E) 和 NIH/3T3 (F) 全细胞裂解液中 p38α MAPK14 表达的 Western 印迹分析.

p38 alpha MAPK14 抗体 (F-9): sc-271120

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寫評論提問

说明书
  • p38 alpha MAPK14抗体(F-9)是小鼠单克隆IgG2b κ, 在41篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的p38α的C-terminus的氨基酸329-360之间
  • p38 alpha MAPK14 抗体 (F-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的p38 alpha MAPK14; 也和以下物种反应，包括： 和 bovine, porcine and avian
  • 抗p38 alpha MAPK14抗体（F-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p38 alpha MAPK14 (F-9): sc-271120的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是p38 alpha MAPK14 抗体 (F-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与p38 alpha MAPK14 抗体 (F-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 p38 MAPK 抗体 包括 p38 alpha MAPK14, p38 beta MAPK11和p38 gamma MAPK12

p38 alpha MAPK14抗体（F-9）是一种IgG2b κ小鼠单克隆p38 alpha MAPK14抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的p38 alpha MAPK14。p38 alpha MAPK14抗体（F-9）既可以是非偶联的抗p38 alpha MAPK14抗体形式，也可以是多种偶联形式的抗p38 alpha MAPK14抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。MAP（丝裂原活化蛋白）激酶在许多生物过程中发挥着重要作用，包括细胞粘附和伸展、细胞分化和凋亡。p38α MAPK14、p38β MAPK11和p38γ MAPK12各包含一个蛋白激酶结构域，属于MAP激酶家族。这些蛋白在体内不同部位表达，在心脏中有共同的表达模式，p38蛋白使用镁作为辅因子来催化ATP依赖性的靶蛋白磷酸化。通过其催化活性，p38α MAPK14、p38β MAPK11和p38γ MAPK12参与细胞内的多种事件，包括信号转导途径、细胞因子产生以及细胞增殖和分化。p38蛋白在Thr和Tyr残基上发生磷酸化，这一事件被认为可以激活磷酸化蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

p38 alpha MAPK14 抗体 (F-9)

sc-271120
200 µg/ml
RMB2377.00

p38 alpha MAPK14 (F-9): m-IgG2b BP-HRP 套装

sc-548694
200 µg Ab; 10 µg BP
RMB2662.00

p38 alpha MAPK14 抗体 (F-9) AC

sc-271120 AC
500 µg/ml, 25% agarose
RMB3129.00

p38 alpha MAPK14 抗体 (F-9) HRP

sc-271120 HRP
200 µg/ml
RMB2377.00

p38 alpha MAPK14 抗体 (F-9) FITC

sc-271120 FITC
200 µg/ml
RMB2482.00

p38 alpha MAPK14 抗体 (F-9) PE

sc-271120 PE
200 µg/ml
RMB2580.00

p38 alpha MAPK14 抗体 (F-9) Alexa Fluor® 488

sc-271120 AF488
200 µg/ml
RMB2685.00

p38 alpha MAPK14 抗体 (F-9) Alexa Fluor® 546

sc-271120 AF546
200 µg/ml
RMB2685.00

p38 alpha MAPK14 抗体 (F-9) Alexa Fluor® 594

sc-271120 AF594
200 µg/ml
RMB2685.00

p38 alpha MAPK14 抗体 (F-9) Alexa Fluor® 647

sc-271120 AF647
200 µg/ml
RMB2685.00

p38 alpha MAPK14 抗体 (F-9) Alexa Fluor® 680

sc-271120 AF680
200 µg/ml
RMB2685.00

p38 alpha MAPK14 抗体 (F-9) Alexa Fluor® 790

sc-271120 AF790
200 µg/ml
RMB2685.00

p38 alpha MAPK14 (F-9) 中和勝肽

sc-271120 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-271120 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Not greatThis antibody showed no detection when tested with mouse liver tissue at 1:500 dilution.
Date published: 2021-12-03
Rated 3 out of 5 by from It does not work wellI used 1:500 dilution when I compare this one with the old one, and this one did not work well
Date published: 2018-04-03
Rated 5 out of 5 by from Specific BindingExcellent antibody with having specific binding, shown exact band at 38 kDa.
Date published: 2017-09-25
Rated 5 out of 5 by from Great for IFThe p38 alpha Antibody (F-9): sc-271120 works very well for immunofluorescence applications, showing clean staining pattern localized to the nucleus. This antibody has also been cited several times for further verification.
Date published: 2017-01-25
Rated 5 out of 5 by from works wellThis antibody works well.Clean band observed in Western Blot analysis in HeLa cellprotein.
Date published: 2017-01-18
Rated 5 out of 5 by from WB: Worked well in mouse bone marrow samplesWB: Worked well in mouse bone marrow samples. -SCBT Publication Review
Date published: 2015-03-26
Rated 5 out of 5 by from Great WB antibodyGreat WB antibody! Published in human RBE and PT67 cell lines. -SCBT Publication Review
Date published: 2015-03-03
Rated 4 out of 5 by from Crisp bands observed in Jurkat and HLCrisp bands observed in Jurkat and HL-60 whole cell lysates for Western Blot. -SCBT QC
Date published: 2015-02-08
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p38 alpha MAPK14 抗体 (F-9) is rated 4.2 out of 5 by 10.
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