Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
P2X7 Antibody (D-1) - Western Blotting - Image 333641
P2X7 抗体 (D-1): sc-514962. Jurkat (A), A-431 (B) 和 SK-N-MC (C) 全细胞裂解液中 P2X7 表达的 Western 印迹分析.
P2X7 抗体 (D-1): sc-514962. Jurkat (A), A-431 (B) 和 SK-N-MC (C) 全细胞裂解液中 P2X7 表达的 Western 印迹分析.

P2X7 抗体 (D-1): sc-514962

4.9(12)
寫評論提問

说明书
  • P2X7 抗体 D-1 是小鼠单克隆 IgG2a κ，P2X7抗体, 在27篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的P2X7的内部区域内的氨基酸81-106之间
  • P2X7 抗体 (D-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 P2X7
  • 抗P2X7抗体（D-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取P2X7 (D-1): sc-514962的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是P2X7 Antibody (D-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与P2X7 Antibody (D-1) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外P2X抗体，包括P2X2, P2X3, P2X5, P2X6和P2X7

P2X7抗体（D-1）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的P2X7。P2X7 (D-1) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。P2X7是P2X受体家族的一员，该家族由配体门控离子通道组成，可促进钙离子在细胞外ATP的作用下流入细胞。P2X7主要位于各种细胞（包括神经元和免疫细胞）的质膜上，在调节突触传递和炎症反应方面发挥着关键作用。P2X7可触发促炎性细胞因子释放，并在ATP浓度过高时参与坏死和凋亡等过程。了解P2X7的功能和位置对于阐明其在各种生理和病理条件（包括神经退行性疾病和慢性炎症）中的作用至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

P2X7 抗体 (D-1) 参考文献:

  1. 细胞溶解 P2X 嘌呤受体。  |  Di Virgilio, F., et al. 1998. Cell Death Differ. 5: 191-9. PMID: 10200464
  2. 西巴克隆蓝对人类 P2X(3)受体的异构调节和加速再敏化。  |  Alexander, K., et al. 1999. J Pharmacol Exp Ther. 291: 1135-42. PMID: 10565834
  3. 二价阳离子对 P2X2 嘌呤受体的失活作用。  |  Ding, S. and Sachs, F. 2000. J Physiol. 522 Pt 2: 199-214. PMID: 10639098
  4. 大鼠视网膜 Müller 细胞中含有 P2X(3), P2X(4), P2X(5) 而非 P2X(7) 嘌呤能受体的证据  |  Jabs, R., et al. 2000. Brain Res Mol Brain Res. 76: 205-10. PMID: 10762695
  5. 因缺失一个氨基酸残基而形成的天然显性阴性 P2X1 受体。  |  Oury, C., et al. 2000. J Biol Chem. 275: 22611-4. PMID: 10816552
  6. 感觉神经元中的 P2X 受体  |  Burnstock, G. 2000. Br J Anaesth. 84: 476-88. PMID: 10823099
  7. 克隆 P2X 受体的药理学。  |  North, RA. and Surprenant, A. 2000. Annu Rev Pharmacol Toxicol. 40: 563-80. PMID: 10836147
  8. P2X7受体在造血和白血病发生中的作用。  |  He, X., et al. 2021. Exp Hematol. 104: 40-47. PMID: 34687808
  9. P2X7受体与慢性疼痛障碍的关系。  |  Ren, WJ. and Illes, P. 2022. Purinergic Signal. 18: 83-92. PMID: 34799827
  10. P2X7受体在粘膜适应性免疫中的作用。  |  Grassi, F. and Marino, R. 2024. Purinergic Signal. 20: 9-19. PMID: 37067746
  11. 解开P2X7中巨孔形成和电流促进的奥秘。  |  Cevoli, F., et al. 2023. Int J Mol Sci. 24: PMID: 37446075
  12. 人类 P2x1 受体：分子克隆, 组织分布和 17 号染色体定位。  |  Longhurst, PA., et al. 1996. Biochim Biophys Acta. 1308: 185-8. PMID: 8809107

订购信息

产品名称产品编号规格价格数量收藏夹

P2X7 抗体 (D-1)

sc-514962
200 µg/ml
RMB2377.00

P2X7 (D-1): m-IgGκ BP-HRP 套装

sc-524886
200 µg Ab, 40 µg BP
RMB2662.00

P2X7 (D-1): m-IgG2a BP-HRP 套装

sc-546176
200 µg Ab; 10 µg BP
RMB2662.00

P2X7 抗体 (D-1) AC

sc-514962 AC
500 µg/ml, 25% agarose
RMB3129.00

P2X7 抗体 (D-1) HRP

sc-514962 HRP
200 µg/ml
RMB2377.00

P2X7 抗体 (D-1) FITC

sc-514962 FITC
200 µg/ml
RMB2482.00

P2X7 抗体 (D-1) PE

sc-514962 PE
200 µg/ml
RMB2580.00

P2X7 抗体 (D-1) Alexa Fluor® 488

sc-514962 AF488
200 µg/ml
RMB2685.00

P2X7 抗体 (D-1) Alexa Fluor® 546

sc-514962 AF546
200 µg/ml
RMB2685.00

P2X7 抗体 (D-1) Alexa Fluor® 594

sc-514962 AF594
200 µg/ml
RMB2685.00

P2X7 抗体 (D-1) Alexa Fluor® 647

sc-514962 AF647
200 µg/ml
RMB2685.00

P2X7 抗体 (D-1) Alexa Fluor® 680

sc-514962 AF680
200 µg/ml
RMB2685.00

P2X7 抗体 (D-1) Alexa Fluor® 790

sc-514962 AF790
200 µg/ml
RMB2685.00

P2X7 (D-1) 中和勝肽

sc-514962 P
100 µg/0.5 ml
RMB511.00

使用这个抗体可以做大鼠脑片石蜡切片的免疫荧光吗？可以提供实验条件吗

Asked by: running
感谢您的提问。非常抱歉，P2X7 抗体 (D-1): sc-514962不推荐用于石蜡切片的免疫荧光实验，冰冻切片是推荐的。谢谢。 Thank you for your question. We are sorry to say that P2X7 Antibody (D-1): sc-514962 is not recommended for immunofluorescence experiments on paraffin sections, frozen sections are recommended . Thank you.
Answered by: Jiajun Z
Date published: 2024-06-19

为什们WB条带主要出现在55KDa

Asked by: lelay
感谢您的提问。您可以拨打电话：021-60936350，发送邮件到[email protected]，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2023-03-08

小鼠海马wb条带出现在55KDa 是否正常？

Asked by: lelay
感谢您的提问。您可以拨打电话：021-60936350，发送邮件到[email protected]，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2023-03-08

Buongiorno, è possibile avere un campione da provare? grazie

Asked by: elena91
Grazie per la tua domanda. Si prega di contattare il nostro reparto di assistenza tecnica (o il distributore locale) per ulteriori informazioni su come ricevere un FREE 10 µg sample.
Answered by: Tech Support Europe
Date published: 2021-10-14

Are you sure it recognizes an intracellular epitope as the description indicates? It appears to me that residues 81-106 of P2X7 are extracellular.

Asked by: Magdiel
Thank you for your inquiry. Anti-P2X7 Antibody (D-1): sc-514962 is raised against an epitope mapping between amino acids 81-106 of human origin. Amino acid 47-334 is located extracellular (https://www.uniprot.org/uniprot/Q99572#family_and_domains).sc-514962 is reocnize the extracellular rigon.
Answered by: Technical Support 9
Date published: 2021-07-14

I would like to know if the specificity of the antibody has been tested e.g. in a KO model.

Asked by: Nayana
Thank you for your question. The specificity of P2X7 Antibody (D-1): sc-514962 has been tested before the release of the antibody. If you are experiencing issues with this antibody, please contact our technical service for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

我想用下你们的小样看看效果，货号sc-514962

Asked by: 张勇zhangyong
感谢您的提问。关于试用装的申请，欢迎通过：电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. For sample request, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-05-09

What application is the blocking peptide sc-514962 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_6, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_8
  • loc_zh_CN, sid_514962, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 107ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from great figureThis product work well in WB at 1:800, liver cell，finally, I obtained clean and beautiful band !
Date published: 2017-11-27
Rated 5 out of 5 by from Results were great.85 kDa specific bands appear when they are diluted with WB 1/500 in HeLa cells. Many papers reported this antibody, and the quality is really good.
Date published: 2017-11-02
Rated 5 out of 5 by from The effect is very goodThis antibody is good for WB, I have a strong band and clean background. I think it's very useful.
Date published: 2017-10-27
Rated 5 out of 5 by from High sensitivityWe used P2X7 antibody got specific staining by cell lysates of human. Not only the stains very brightly but also the signal is powerful.
Date published: 2017-10-16
Rated 5 out of 5 by from It works great!I got a free sample and it worked great for western blot using rat brain proteins.
Date published: 2017-08-07
Rated 5 out of 5 by from effect is goodP2X7 antibody effect is good, immunohistochemical staining uniform, positive coloring significantly.
Date published: 2017-07-21
Rated 5 out of 5 by from Specific resultsWe have used it for WB analysis in spinal cord of rats: this antibody test sample was perfect, strong and very specific results!
Date published: 2017-06-29
Rated 5 out of 5 by from Popular antibodyVery good antibody WB detection P2X7 receptor analysis, welcomed by the laboratory people.
Date published: 2017-06-01
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_514962, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
P2X7 抗体 (D-1) is rated 4.9 out of 5 by 12.
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_514962, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 107ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。