Date published: 2025-9-8
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CDKN2A/p19ARF Antibody (5-C3-1) - Western Blotting - Image 378741
p19 ARF 抗体 (5-C3-1): sc-32748. F9 (A), TK-1 (B) 和 BW5147 (C) 全细胞裂解液中 p19 ARF 表达的 Western 印迹分析.
p19 ARF 抗体 (5-C3-1): sc-32748. F9 (A), TK-1 (B) 和 BW5147 (C) 全细胞裂解液中 p19 ARF 表达的 Western 印迹分析.

CDKN2A/p19ARF 抗体 (5-C3-1): sc-32748

4.3(9)
寫評論提問

说明书
  • CDKN2A/p19ARF 抗体 5-C3-1 是大鼠单克隆 IgG2b，CDKN2A/p19ARF抗体, 在98篇文献中引用，规格为200 µg/ml
  • 与两种肽的混合物结合，这两种肽分别对应于和mouse来源的p19 ARF的156-169个氨基酸；表位图谱对应于62-75个氨基酸
  • CDKN2A/p19ARF 抗体 (5-C3-1) 推荐用于 WB, IP 和 IF，检测mouse 和rat 来源的 p19 ARF; 不会与 p19 ARF of human or golden hamster origin产生交联反应
  • 抗CDKN2A/p19ARF抗体（5-C3-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CDKN2A/p19ARF (5-C3-1): sc-32748的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CDKN2A/p19ARF 抗体 (5-C3-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 p16 INK4A/p19 ARF/p14 ARF (CDKN2A) 抗体 包括 p19 ARF

p19 ARF抗体（5-C3-1）是一种鼠单克隆IgG2b抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测小鼠和大鼠来源的p19 ARF蛋白。p19 ARF抗体（5-C3-1）有非结合型和多种结合型，包括 琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。p19 ARF作为肿瘤抑制因子，在调节细胞周期中发挥着至关重要的作用，尤其是通过与p53途径相互作用。p19 ARF对于维持基因组稳定性和防止细胞无序增殖至关重要，而细胞无序增殖会导致肿瘤形成。值得注意的是，p19 ARF源自p16基因的另一个读码框架，虽然与p16不同，但具有诱导细胞周期停滞的能力。研究表明，尽管小鼠的p16基因功能正常，但缺少p19 ARF的小鼠会在幼年时患上肿瘤，这凸显了p19 ARF在预防癌症方面的重要作用。p19 ARF表达的紊乱与人类T细胞急性淋巴细胞白血病有很大关系，因此p19 ARF成为癌症生物学和治疗开发研究的重要目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CDKN2A/p19ARF 抗体 (5-C3-1) 参考文献:

  1. 小鼠 p19（Arf）肿瘤抑制蛋白的单克隆抗体。  |  Bertwistle, D., et al. 2004. Hybrid Hybridomics. 23: 293-300. PMID: 15672607
  2. 细胞对慢性 DNA 损伤的反应需要 p19(Arf)。  |  Bieging-Rolett, KT., et al. 2016. Oncogene. 35: 4414-21. PMID: 26725325
  3. p19(Arf)限制PDGF-B驱动的原发性玻璃体细胞增殖。  |  Iqbal, NS., et al. 2016. Exp Eye Res. 145: 224-229. PMID: 26778750
  4. p19Arf 会加剧香烟烟雾诱发的肺功能障碍  |  Mikawa, R., et al. 2020. Biomolecules. 10: PMID: 32192082
  5. 一种新型 p16INK4A 转录本。  |  Mao, L., et al. 1995. Cancer Res. 55: 2995-7. PMID: 7541708
  6. 一种细胞周期调节剂，可能与多种肿瘤类型的发生有关。  |  Kamb, A., et al. 1994. Science. 264: 436-40. PMID: 8153634
  7. 制动循环。  |  Hunter, T. 1993. Cell. 75: 839-41. PMID: 8252620
  8. 细胞周期控制中的一种新的调节基因，可对细胞周期蛋白 D/CDK4 产生特异性抑制作用。  |  Serrano, M., et al. 1993. Nature. 366: 704-7. PMID: 8259215
  9. 哺乳动物 G1 细胞周期蛋白  |  Sherr, CJ. 1993. Cell. 73: 1059-65. PMID: 8513492
  10. INK4a 肿瘤抑制基因的替代阅读框编码两种能够诱导细胞周期停滞的不相关蛋白。  |  Quelle, DE., et al. 1995. Cell. 83: 993-1000. PMID: 8521522
  11. 由替代阅读框产品 p19ARF 介导的小鼠 INK4a 基因座的肿瘤抑制作用。  |  Kamijo, T., et al. 1997. Cell. 91: 649-59. PMID: 9393858
  12. T 细胞急性淋巴细胞白血病中 p19ARF 编码外显子的基因组改变。  |  Gardie, B., et al. 1998. Blood. 91: 1016-20. PMID: 9446664

订购信息

产品名称产品编号规格价格数量收藏夹

CDKN2A/p19ARF 抗体 (5-C3-1)

sc-32748
200 µg/ml
RMB2377.00

CDKN2A/p19ARF 抗体 (5-C3-1) AC

sc-32748 AC
500 µg/ml, 25% agarose
RMB3129.00

CDKN2A/p19ARF 抗体 (5-C3-1) HRP

sc-32748 HRP
200 µg/ml
RMB2377.00

CDKN2A/p19ARF 抗体 (5-C3-1) FITC

sc-32748 FITC
200 µg/ml
RMB2482.00

CDKN2A/p19ARF 抗体 (5-C3-1) PE

sc-32748 PE
200 µg/ml
RMB2580.00

CDKN2A/p19ARF 抗体 (5-C3-1) Alexa Fluor® 488

sc-32748 AF488
200 µg/ml
RMB2685.00

CDKN2A/p19ARF 抗体 (5-C3-1) Alexa Fluor® 546

sc-32748 AF546
200 µg/ml
RMB2685.00

CDKN2A/p19ARF 抗体 (5-C3-1) Alexa Fluor® 594

sc-32748 AF594
200 µg/ml
RMB2685.00

CDKN2A/p19ARF 抗体 (5-C3-1) Alexa Fluor® 647

sc-32748 AF647
200 µg/ml
RMB2685.00

CDKN2A/p19ARF 抗体 (5-C3-1) Alexa Fluor® 680

sc-32748 AF680
200 µg/ml
RMB2685.00

CDKN2A/p19ARF 抗体 (5-C3-1) Alexa Fluor® 790

sc-32748 AF790
200 µg/ml
RMB2685.00

Which santa CRuz secondary antibody do you recomen for p19 ARF (5-C3-1) detection by western blot? I have not found any anti-rat IgG-HRP.

Asked by: LGonzalez
Thank you for your question. This is a rat monoclonal IgG2b, and we recommend using a secondary antibody that is anti-rat IgG. Unfortunately, we do not currently offer any secondary antibodies specific for detection of rat IgG. Please check back for updates!
Answered by: Technical Support
Date published: 2019-07-20

32748, I need 10ug sample to test on my brancio cephalic artey from mouse orgin. I can use alexa-488/546/647 or unconjugated. I also requested for p16 377412. I will order once I validate these 2 antibodies.Dr Santosh, Gary Owens lab UVA

Asked by: p16 and p19
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

What is the epitope recognized by p19 ARF (5-C3-1)?

Asked by: cjMara
p19 ARF Antibody (5-C3-1) has an epitope mapping to amino acids 62-75 of p19 ARF of mouse origin.
Answered by: Technical Support 9
Date published: 2017-01-09
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Rated 5 out of 5 by from Worked GreatTested a sample for our lung tumor cells. Used mouse testes as positive control. Worked great. Ordering now.
Date published: 2017-11-30
Rated 4 out of 5 by from great for western blotIt is stable in WB, and the result is very good.Good function.
Date published: 2017-02-11
Rated 5 out of 5 by from Strong antibodyStrong signal obtained in Western blot, and the antibody works well in mouse or rat samples for p19 ARF detection.
Date published: 2017-01-18
Rated 5 out of 5 by from Superb!!Very stong western blot data;low background; also nice FA
Date published: 2017-01-09
Rated 4 out of 5 by from Very good antibody with no backgroundI used this on lung epithelial cells and the blot is beautiful with no background. Used 1:500 and 1:20k for secondary.
Date published: 2016-10-06
Rated 5 out of 5 by from worked excellent for WB analysis on mouse lysatesworked excellent for WB analysis on mouse lysates
Date published: 2016-07-28
Rated 3 out of 5 by from Robust bands seen in WB using NIH/3T3 wholeRobust bands seen in WB using NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-03-24
Rated 5 out of 5 by from Produced superb immunofluorescence nuclearProduced superb immunofluorescence nuclear staining in wtMEFp6 cells. -SCBT QC
Date published: 2015-03-12
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CDKN2A/p19ARF 抗体 (5-C3-1) is rated 4.3 out of 5 by 9.
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