Date published: 2025-9-8
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p16-ARC Antibody (C-3) - Western Blotting - Image 362499
p16-ARC 抗体 (C-3): sc-166760. 在 HeLa (A), Hep G2 (B), U-87 MG (C), HL-60 (D), HT-1080 (E) 和 DU 145 (F) 全细胞裂解液中 p16-ARC 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
p16-ARC 抗体 (C-3): sc-166760. 在 HeLa (A), Hep G2 (B), U-87 MG (C), HL-60 (D), HT-1080 (E) 和 DU 145 (F) 全细胞裂解液中 p16-ARC 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

p16-ARC 抗体 (C-3): sc-166760

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寫評論提問

说明书
  • p16-ARC 抗体 C-3 是小鼠单克隆 IgG2b κ，p16-ARC抗体, 在22篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长p16-ARC的氨基酸1-151
  • p16-ARC 抗体 (C-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 p16-ARC
  • 抗p16-ARC抗体（C-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p16-ARC (C-3): sc-166760的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是p16-ARC Antibody (C-3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与p16-ARC Antibody (C-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加p ARC抗体，包括p16-ARC, p21-ARC, p34-ARC和p41-ARCb

p16-ARC抗体（C-3）是一种IgG2b κ小鼠单克隆p16-ARC抗体（也称为p16-ARC抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的p16-ARC蛋白。p16-ARC抗体（C-3）既可以是非偶联的抗p16-ARC抗体形式，也可以是多种偶联形式的抗p16-ARC抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Arp2/3（肌动蛋白相关蛋白2/3）复合物由七个亚基组成，所有这些都是与肌动蛋白相关的蛋白质。该复合物参与肌动蛋白聚合的控制并介导分支肌动蛋白网络的形成。p16-ARC，也称为ARPC5（肌动蛋白相关蛋白2/3复合物亚基5）或ARC16（Arp2/3复合物16 kDa亚基），是Arp2/3复合物的151个氨基酸亚基。p16-ARC被认为在维持Arp2/3的完整性中发挥作用，它是MAPKAPK-2的底物，MAPKAPK-2通过磷酸化p16-ARC可能参与Arp2/3的调节功能和肌动蛋白细胞骨架的重塑。由于选择性剪接事件，存在两种p16-ARC异构体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p16-ARC 抗体 (C-3) 参考文献:

  1. 人类 Arp2/3 复合物亚基之间的相互作用：p20-Arc 是枢纽。  |  Zhao, X., et al. 2001. Biochem Biophys Res Commun. 280: 513-7. PMID: 11162547
  2. 人类 Arp2/3 复合物的重建揭示了单个亚基在复合物结构和活性中的关键作用。  |  Gournier, H., et al. 2001. Mol Cell. 8: 1041-52. PMID: 11741539
  3. 鸡组织中 Arp2/3 复合物的特征。  |  Terasaki, AG., et al. 2002. Cell Struct Funct. 27: 383-91. PMID: 12502893
  4. 通过蛋白质组分析确定 Arp 2/3 复合物的 p16-Arc 亚基是 MAPK 激活的蛋白激酶 2 的底物。  |  Singh, S., et al. 2003. J Biol Chem. 278: 36410-7. PMID: 12829704
  5. Arp2/3 复合物形成肌动蛋白丝分支的途径  |  Beltzner, CC. and Pollard, TD. 2008. J Biol Chem. 283: 7135-44. PMID: 18165685
  6. 人类 Arp2/3 复合物由进化保守的亚基组成，定域于动态肌动蛋白丝组装的细胞区域。  |  Welch, MD., et al. 1997. J Cell Biol. 138: 375-84. PMID: 9230079
  7. 哺乳动物肌动蛋白相关蛋白 2/3 复合物定位于薄片突起区域，由进化保守的蛋白质组成。  |  Machesky, LM., et al. 1997. Biochem J. 328 (Pt 1): 105-12. PMID: 9359840
  8. Arp2/3 复合物亚基 p16-ARC/ARPC5 的新型人类异构体的鉴定和特征描述  |  Millard, TH., et al. 2003. Cell Motil Cytoskeleton. 54: 81-90. PMID: 12451597

订购信息

产品名称产品编号规格价格数量收藏夹

p16-ARC 抗体 (C-3)

sc-166760
200 µg/ml
RMB2377.00

p16-ARC (C-3): m-IgGκ BP-HRP 套装

sc-521734
200 µg Ab, 40 µg BP
RMB2662.00

p16-ARC (C-3): m-IgG2b BP-HRP 套装

sc-548962
200 µg Ab; 10 µg BP
RMB2662.00

p16-ARC 抗体 (C-3) AC

sc-166760 AC
500 µg/ml, 25% agarose
RMB3129.00

p16-ARC 抗体 (C-3) HRP

sc-166760 HRP
200 µg/ml
RMB2377.00

p16-ARC 抗体 (C-3) FITC

sc-166760 FITC
200 µg/ml
RMB2482.00

p16-ARC 抗体 (C-3) PE

sc-166760 PE
200 µg/ml
RMB2580.00

p16-ARC 抗体 (C-3) Alexa Fluor® 488

sc-166760 AF488
200 µg/ml
RMB2685.00

p16-ARC 抗体 (C-3) Alexa Fluor® 546

sc-166760 AF546
200 µg/ml
RMB2685.00

p16-ARC 抗体 (C-3) Alexa Fluor® 594

sc-166760 AF594
200 µg/ml
RMB2685.00

p16-ARC 抗体 (C-3) Alexa Fluor® 647

sc-166760 AF647
200 µg/ml
RMB2685.00

p16-ARC 抗体 (C-3) Alexa Fluor® 680

sc-166760 AF680
200 µg/ml
RMB2685.00

p16-ARC 抗体 (C-3) Alexa Fluor® 790

sc-166760 AF790
200 µg/ml
RMB2685.00

What's the difference between p16-ARC and CDKN2A/p16INK4a? Can this antibody be used as a marker of senescence?

Asked by: Lexie
感谢提问。 这两个货号抗体是两种不同的靶标的抗体。 这两个货号推荐的实验方法以及种属反应，见官网。 https://www.scbt.com/p/p16-arc-antibody-c-3?requestFrom=search https://www.scbt.com/p/p16-antibody-f-12?requestFrom=search sc-166760，Anti-p16-ARC Antibody (C-3) is recommended for detection of p16-ARC of mouse, rat and human origin by WB, IP, IF and ELISA sc-1661，Anti-CDKN2A/p16INK4a Antibody (F-12) is recommended for detection of p16 INK4A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA Thank you for your question. They are antibodies with different protein. The applications about the two antibodies we can check on our official website. https://www.scbt.com/p/p16-arc-antibody-c-3?requestFrom=search https://www.scbt.com/p/p16-antibody-f-12?requestFrom=search sc-166760，Anti-p16-ARC Antibody (C-3) is recommended for detection of p16-ARC of mouse, rat and human origin by WB, IP, IF and ELISA sc-1661，Anti-CDKN2A/p16INK4a Antibody (F-12) is recommended for detection of p16 INK4A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Answered by: Ling Chen
Date published: 2024-04-01

Do you know if your p16 antibody 166760 has been used in flow cytometry?

Asked by: Jolly
Thank you for your question. We are aware of this antibody being published for use in flow cytometry (FCM). Please see PMID: 30082068 as a reference. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-08-17

What is the recommended fixation method for immunofluorescence staining with p16-ARC (C-3): sc-166760 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Good results in immunofluorescenceThe antibody works quite well in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA
Date published: 2020-11-10
Rated 5 out of 5 by from the antibody is wellthe antibody is well,i used it to do IHC,dilution in 1:50
Date published: 2017-12-28
Rated 5 out of 5 by from This p16 antibody works well！This p16 antibody works well without nonspecific bands.
Date published: 2017-10-27
Rated 5 out of 5 by from Highly recommendedIt has signals in the tissues of mice, and it's very clear, play an important role in cancer cell lysates, It's worth recommending and buying.
Date published: 2017-10-18
Rated 5 out of 5 by from magnificentThis antibody works well when we use 1:2000 dilution in WB, and our results shows a beautiful brand.
Date published: 2017-09-20
Rated 4 out of 5 by from sometimes workI use tissue to do Western blot. and sometimes it's work and sometimes doesn't work.
Date published: 2017-08-19
Rated 5 out of 5 by from very goodStrong band with clean background in western blot with HL-60 whole cell lysate (1/500)
Date published: 2016-12-17
Rated 5 out of 5 by from IF publishable data with rat brainIF publishable data with rat brain - SCBT Publication Review
Date published: 2015-02-03
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p16-ARC 抗体 (C-3) is rated 4.7 out of 5 by 9.
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