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CDKN2B/CDKN2A/p16 Antibody (C-7) - Western Blotting - Image 361612
p15 INK4B/p16 INK4A 抗体 (C-7): sc-377412. HeLa (A) 和 Saos-2 (B) 全细胞裂解液中 p15 INK4B/p16 INK4A 表达的 Western 印迹分析.
p15 INK4B/p16 INK4A 抗体 (C-7): sc-377412. HeLa (A) 和 Saos-2 (B) 全细胞裂解液中 p15 INK4B/p16 INK4A 表达的 Western 印迹分析.

CDKN2B/CDKN2A/p16 抗体 (C-7): sc-377412

4.1(21)
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说明书
  • CDKN2B/CDKN2A/p16抗体(C-7)是小鼠单克隆IgG1 κ, 在53篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列96-138位于human物种的p15 INK4B的C-terminus
  • 抗-CDKN2B/CDKN2A/p16 抗体 (C-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 p15 INK4B and p16 INK4A
  • 抗CDKN2B/CDKN2A/p16抗体（C-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CDKN2B/CDKN2A/p16 (C-7): sc-377412的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 CDKN2B/CDKN2A/p16 抗体 (C-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与CDKN2B/CDKN2A/p16 抗体 (C-7) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 p15 INK4B 抗体包括 PCLAF

p15 INK4B/p16 抗体 (C-7) 是一种IgG1 κ小鼠单克隆p15 INK4B/p16 INK4A抗体（也称为p15 INK4B/p16 INK4A抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人的p15 INK4B和p16 INK4A。p15 INK4B/p16 抗体 (C-7)既可以是非偶联的抗p15 INK4B/p16 INK4A抗体形式，也可以是多种偶联形式的抗p15 INK4B/p16 INK4A抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞通过细胞周期的正常进程受到细胞周期蛋白依赖性蛋白激酶Cdk4和Cdk6的控制，这两种激酶受到有丝分裂抑制蛋白p16 INK4A的抑制。Cdk4与D型细胞周期蛋白形成的复合物在G1期细胞增殖的控制中起着重要作用。已表明p16 INK4A与Cdk4结合并抑制Cdk4/cyclin D复合物的催化活性。此外，在已建立的人肿瘤细胞系中，编码p16 INK4A的基因表现出高频率的纯合缺失和点突变。p15 INK4B（也称为p15、INK4B、CDK4I、TP15或MTS2）是p16 INK4A家族的成员，其在经TGFβ处理的人角质形成细胞中的表达上调约30倍，表明p15 INK4B可能作为TGFβ介导的细胞周期停滞的效应器。编码p15 INK4B的基因（CDKN2B）已被定位于染色体9p21.3上，紧邻p16 INK4A基因，这是人类肿瘤中染色体异常频繁发生的位点。有人提出，p15 INK4B可能通过抑制Cdk4和Cdk6激酶来作为TGFβ介导的细胞周期停滞的效应器。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CDKN2B/CDKN2A/p16 抗体 (C-7)

sc-377412
200 µg/ml
RMB2377.00

CDKN2B/CDKN2A/p16 (C-7): m-IgGκ BP-HRP 套装

sc-523320
200 µg Ab, 40 µg BP
RMB2662.00

CDKN2B/CDKN2A/p16 抗体 (C-7) AC

sc-377412 AC
500 µg/ml, 25% agarose
RMB3129.00

CDKN2B/CDKN2A/p16 抗体 (C-7) HRP

sc-377412 HRP
200 µg/ml
RMB2377.00

CDKN2B/CDKN2A/p16 抗体 (C-7) FITC

sc-377412 FITC
200 µg/ml
RMB2482.00

CDKN2B/CDKN2A/p16 抗体 (C-7) PE

sc-377412 PE
200 µg/ml
RMB2580.00

CDKN2B/CDKN2A/p16 抗体 (C-7) Alexa Fluor® 488

sc-377412 AF488
200 µg/ml
RMB2685.00

CDKN2B/CDKN2A/p16 抗体 (C-7) Alexa Fluor® 546

sc-377412 AF546
200 µg/ml
RMB2685.00

CDKN2B/CDKN2A/p16 抗体 (C-7) Alexa Fluor® 594

sc-377412 AF594
200 µg/ml
RMB2685.00

CDKN2B/CDKN2A/p16 抗体 (C-7) Alexa Fluor® 647

sc-377412 AF647
200 µg/ml
RMB2685.00

CDKN2B/CDKN2A/p16 抗体 (C-7) Alexa Fluor® 680

sc-377412 AF680
200 µg/ml
RMB2685.00

CDKN2B/CDKN2A/p16 抗体 (C-7) Alexa Fluor® 790

sc-377412 AF790
200 µg/ml
RMB2685.00

DOES THIS ANTIBODY DETECT P16 PROTEIN IN MOUSE OR HUMAN CELLS?

Asked by: ELIZABETHJG
Thank you for your question. This antibody, p15/p16 (C-7), sc-377412, is both human and mouse reactive, so it will detect p15/16 in both mouse and human cells.
Answered by: Tech Service
Date published: 2019-12-16

I'm getting a band at roughly 24kDa for whole mouse bone marrow lysate with this antibody. I'm not getting any bands near 15 or 16kDa. Do you have any recommendations? I've run several westerns with the same result

Asked by: yim123
Thank you for your question. We would recommend including a positive control, such as 3T3-L1 whole cell lysate (sc-2243) for comparison and to help you troubleshoot the antibody. We would recommend using the primary antibody at a starting dilution of 1:200, and loading 40-60 µg of protein. A link to our complete recommended protocol for Western blotting with this antibody is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting Please contact our Technical Service Department and we are happy to help you troubleshoot the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-11-02

p15/p16 (C-7): sc-377412의 무료10 µg 샘플을 신청합니다.

Asked by: junguee
산타크루즈바이오테크놀러지에 문의 주셔서 감사합니다.sc-377412 무료10 µg 샘플이 있습니다. 신청하려면 우리 회사 한국에 있는 distributor에게 연락해 주십시오.아래는 연락처입니다. 다른 문의사항은 언제든지 메일로 기술지원팀에게 보내주시기 바랍니다 We have sc-377412 free sample for test.You can contact your local distributor to request SCBT antibody sample.Below is the address of our distributor in Korea.Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions. Your local distributor(s): Essence Medical Inc. 417 Leaders B/D, Yatapdong 342-1 Bundanggu Seongnam Gyeonggi-do Korea Tel. 82-31-707-1544 FAX: 82-31-707-1999 Email: [email protected] Website: http://www.essencemedical.com Koram Biotech Corporation 6F, Koram Venture Building 907-1 Daechi-Dong Kang Nam-Ku Seoul 135-080 Korea Tel. 82 (2) 556-0311 FAX: 82 (2) 556-0828 Email: [email protected] Website: http://www.korambiotech.com
Answered by: Technical Service 11
Date published: 2017-08-17

Can this antibody be used to detect p16 in canine samples?

Asked by: AbPolly
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service
Date published: 2016-12-22
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Rated 4 out of 5 by from goodUsed for Immunoblotting 1:500 in tbs 0.1% tween overnight on DU145 extract, it produces a specific signal at the expected molecular weight on WB.
Date published: 2021-10-04
Rated 2 out of 5 by from Didn't workUnfortunately we couldn't make it to work on our rat cell line (western blot).
Date published: 2018-08-01
Rated 3 out of 5 by from Works well for WB but not for IFWB works at a dilution of 1:250 on neonatal mouse lung lysates. Protein loaded 30ug
Date published: 2017-12-18
Rated 1 out of 5 by from P16 Thumbs downDidn't work for me unfortunately even at 1:100 in A2780 and SKOV-3 Ovarian Cancer cell lysates, despite success using GAPDH
Date published: 2017-11-29
Rated 5 out of 5 by from Works well for WB but not for IFCan be used at a dilution of 1:200 for WB on mouse lung lysates with 30ug protein loaded
Date published: 2017-11-08
Rated 5 out of 5 by from the result satisfiedThe researchers confirmed that knowledge of the sensitivity and specificity of p15/p16 Antibody leads to their more rational selection.
Date published: 2017-10-21
Rated 4 out of 5 by from Good antibody for IBThis antibody works well in the immunoblotting assay. Meanwhile, unspecific bands could also be detected.
Date published: 2017-10-08
Rated 5 out of 5 by from does not work1:500 diluted for wb of HL-60 and MOLM13 protein,there is no band
Date published: 2017-09-19
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CDKN2B/CDKN2A/p16 抗体 (C-7) is rated 4.1 out of 5 by 21.
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