Date published: 2025-9-11
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Phosphotyrosine Antibody (PY99) - Immunohistochemistry - Image 348953
p-Tyr 抗体 (PY99): sc-7020. 福尔马林固定, 石蜡包埋的正常人皮肤组织的免疫过氧化物酶染色, 显示酪氨酸磷酸化蛋白的膜和细胞质定位.
p-Tyr 抗体 (PY99): sc-7020. 福尔马林固定, 石蜡包埋的正常人皮肤组织的免疫过氧化物酶染色, 显示酪氨酸磷酸化蛋白的膜和细胞质定位.

Phosphotyrosine 抗体 (PY99): sc-7020

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说明书
  • Phosphotyrosine抗体(PY99)是小鼠单克隆IgG2b κ, 在2,689篇文献中引用，规格为200 µg/ml
  • 针对p-Tyr
  • Phosphotyrosine 抗体 (PY99) 推荐用于 WB, IP, IF 和 IHC(P)，检测 phosphotyrosine-containing proteins ; 不会与 phosphoserine or phosphothreonine产生交联反应
  • 抗Phosphotyrosine抗体（PY99）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素可用于WB、IHC(P)和ELISA实验；以及Alexa Fluor® 405 用于IF、IHC(P)和FCM实验
  • 关于如何获取Phosphotyrosine (PY99): sc-7020的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Phosphotyrosine 抗体 (PY99)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
说明书与实验方案
研究信息

関連項目

此外 p-Tyr 抗体包括 p-Ser 和 p-Thr

p-Tyr抗体（PY99）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测多种物种的磷酸化酪氨酸蛋白。p-Tyr（PY9 9）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®选项，可满足各种实验需求。蛋白质酪氨酸磷酸化在细胞信号传导通路中起着至关重要的作用，是细胞生长、分化和新陈代谢等众多生物过程的关键调节机制。酪氨酸残基的磷酸化主要通过蛋白酪氨酸激酶发生，包括受体酪氨酸激酶和非受体酪氨酸激酶，其中Src家族是后者的典型代表。这些激酶通常定位于质膜的内表面，与表面受体相互作用，在细胞内传递信号。当受体酪氨酸激酶与配体结合时，它们会发生二聚化和自磷酸化，形成特定的磷酸化酪氨酸位点，与下游信号分子对接。这个复杂的网络凸显了研究磷酸化酪氨酸修饰的重要性，使得抗磷酸化酪氨酸抗体（PY99）对于研究并纯化含有磷酸化酪氨酸残基的蛋白质的研究人员来说至关重要，最终推动了细胞信号传导研究的发展。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Phosphotyrosine 抗体 (PY99) 参考文献:

  1. 使用抗磷酸化酪氨酸抗体纯化血小板衍生生长因子受体。  |  Daniel, TO., et al. 1985. Proc Natl Acad Sci U S A. 82: 2684-7. PMID: 2581254
  2. 艾贝尔森鼠白血病病毒编码的蛋白酪氨酸激酶的纯化和特征。  |  Foulkes, JG., et al. 1985. J Biol Chem. 260: 8070-7. PMID: 2989275
  3. 人脑脊液的磷酸酪氨酸谱分析  |  Sathe, G., et al. 2018. Clin Proteomics. 15: 29. PMID: 30220890
  4. 阻断磷酸化酪氨酸途径表明SH2超级结合剂是治疗肺纤维化的一种新疗法。  |  Wang, M., et al. 2022. Theranostics. 12: 4513-4535. PMID: 35832075
  5. 肿瘤启动子阻断了表皮生长因子受体的酪氨酸特异性磷酸化。  |  Friedman, B., et al. 1984. Proc Natl Acad Sci U S A. 81: 3034-8. PMID: 6328489
  6. 受体酪氨酸激酶的信号传递。  |  Fantl, WJ., et al. 1993. Annu Rev Biochem. 62: 453-81. PMID: 7688944
  7. 受体寡聚化对信号转导和信号多样性的调节。  |  Lemmon, MA. and Schlessinger, J. 1994. Trends Biochem Sci. 19: 459-63. PMID: 7855887
  8. 将白细胞 GPI 锚定蛋白和蛋白酪氨酸激酶纳入 COS-7 细胞的富脂膜域。  |  Cebecauer, M., et al. 1998. Biochem Biophys Res Commun. 243: 706-10. PMID: 9500981
  9. T 细胞受体信号导致抗洗涤剂膜微域中的链接蛋白 LAT 快速发生酪氨酸磷酸化。  |  Brdiĉka, T., et al. 1998. Biochem Biophys Res Commun. 248: 356-60. PMID: 9675140

订购信息

产品名称产品编号规格价格数量收藏夹

Phosphotyrosine 抗体 (PY99)

sc-7020
200 µg/ml
RMB2377.00

Phosphotyrosine 抗体 (PY99) AC

sc-7020 AC
500 µg/ml, 25% agarose
RMB3129.00

Phosphotyrosine 抗体 (PY99) HRP

sc-7020 HRP
200 µg/ml
RMB2377.00

Phosphotyrosine 抗体 (PY99) FITC

sc-7020 FITC
200 µg/ml
RMB2482.00

Phosphotyrosine 抗体 (PY99) PE

sc-7020 PE
200 µg/ml
RMB2580.00

Phosphotyrosine 抗体 (PY99) Alexa Fluor® 488

sc-7020 AF488
200 µg/ml
RMB2685.00

Phosphotyrosine 抗体 (PY99) Alexa Fluor® 546

sc-7020 AF546
200 µg/ml
RMB2685.00

Phosphotyrosine 抗体 (PY99) Alexa Fluor® 594

sc-7020 AF594
200 µg/ml
RMB2685.00

Phosphotyrosine 抗体 (PY99) Alexa Fluor® 647

sc-7020 AF647
200 µg/ml
RMB2685.00

Phosphotyrosine 抗体 (PY99) Alexa Fluor® 680

sc-7020 AF680
200 µg/ml
RMB2685.00

Phosphotyrosine 抗体 (PY99) Alexa Fluor® 790

sc-7020 AF790
200 µg/ml
RMB2685.00

Phosphotyrosine 抗体 (PY99) B

sc-7020 B
200 µg/ml
RMB2407.00

Phosphotyrosine 抗体 (PY99) AP

sc-7020 AP
200 µg/ml

What's the specific phosphorylation site of TEK does this p-Tyr (PY99) combine?

Asked by: xxxxxi
Thank you for your question. Phosphotyrosine Antibody (PY99): sc-7020 is raised against p-Tyr, the specific binding site of this antibody was not characterized.
Answered by: Technical Service SH
Date published: 2023-07-24

What dilutions do you recommend for both the primary and secondary antibodies in .5% BSA for Western Blotting.

Asked by: carolineh325
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-12-19

Is there anybody know the difference between PY20 and PY99?

Asked by: shaw
Thank you for your question. p-Tyr Antibody (PY99) is recommended for detection of phosphotyrosine-containing proteins by WB, IP, IF and IHC(P); non cross-reactive with phosphoserine or phosphothreonine. p-Tyr Antibody (PY20) is recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM.
Answered by: Technical Support 12
Date published: 2022-06-17

Dear Santa Cruze Do i have to boil/unboil my cell lysate before i use this p-Tyr(PY99) for western blot application?

Asked by: Kenny C
Thank you for your question. Yes, you should boil your lysate before loading it onto your gel.
Answered by: Tech Service
Date published: 2019-11-25

When I use this antibody for immunohistochemistry. Is antigen retrieval necessary?

Asked by: AH2019
Thank you for your question. Yes, we recommend performing an antigen retrieval step if you are doing IHC on formalin fixed paraffin embedded tissue sections.
Answered by: Tech Service
Date published: 2019-10-15

Can I use 10% milk to block when using this antibody? I am interested in getting it conjugated with the alexa 790 to be used with LI-COR®Odyssey®.

Asked by: nsal
Thank you for your question. We recommend blocking in 5% milk with TBS-T, and it should be fine with the LiCOR Odyssey.
Answered by: Tech Service
Date published: 2019-09-24

Is there any species specificity for this antibody? Or is there any cross-reactivity?

Asked by: beni
Thank you for your question. This antibody has broad species reactivity and is specific for phosphotyrosine-containing proteins; non cross-reactive with phosphoserine or phosphothreonine.
Answered by: Technical Support
Date published: 2019-09-11

I want to measure insulin sensitivity and several tyrosine domains are phosphorylated in response to insulin. For example in both insulin receptors and in IRS. Does the antibody detetct all p-tyr, and I differentiate between them depending on weight?

Asked by: Ahmed_hassan
Thank you for your question. Yes, you are correct, this mouse monoclonal antibody, p-Tyr (PY99): sc-7020, specifically detects phosphotyrosine-containing proteins (and does not cross-reactive with phosphoserine or phosphothreonine-containing proteins). If you are using the antibody for Western blotting, then the molecular weight you detect in your samples is representative of different phosphorylated proteins. You would need to do further testing to identify which specific proteins are phosphorylated in your samples.
Answered by: Technical Support
Date published: 2021-04-06
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Rated 3 out of 5 by from Pretty good antibodyThe antibody works pretty well upon IP whereas on total cellular extract is too weak.
Date published: 2020-10-07
Rated 5 out of 5 by from detection of phosphotyrosine-containing proteinsI recommend for detection of phosphotyrosine-containing proteins!
Date published: 2019-12-04
Rated 4 out of 5 by from good pTr antibodyWorks well to detect tyrosine phosphorylation in whole cell or tissue lysates.
Date published: 2018-05-17
Rated 5 out of 5 by from good for WBI use 1:1000 in 5%BSA, it is less specific for mouse lung tissue, I haven't try primary cell but I hope it works.
Date published: 2018-03-12
Rated 4 out of 5 by from Good for WBI tried a 1/500 dilution in 3% BSA and it worked well. Good signal.
Date published: 2017-10-24
Rated 5 out of 5 by from IHC detectionIHC detected the expression of P-Tyr in gastric carcinoma tissues ,it gave cytoplasmic staining ,good signal for us.
Date published: 2017-09-16
Rated 5 out of 5 by from stainingIHC(1/100) staining in human skin tissu, very sensitive
Date published: 2017-09-08
Rated 5 out of 5 by from very goodGives clear for nuclear extracts in western blot; had a strong signal in the western blot.
Date published: 2017-08-17
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Phosphotyrosine 抗体 (PY99) is rated 4.7 out of 5 by 31.
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