Date published: 2025-9-6
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p-Smad3 抗体 (1D9): sc-517575

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说明书
  • p-Smad3 抗体 1D9 是小鼠单克隆 IgG, 在33篇文献中引用，提供 100 µg/ml
  • 针对human起源的Ser 425磷酸化Smad3
  • 推荐用于 mouse, rat 和 human 来源的Ser 425 phosphorylated Smad3 IF 和 IHC(P)检测
  • 关于如何获取p-Smad3 (1D9): sc-517575的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是p-Smad3 Antibody (1D9) for IHC(P) applications. 的首选辅助检测试剂，这些试剂现与p-Smad3 Antibody (1D9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Smad抗体，包括Smad1, Smad2, Smad3, Smad4, Smad5, Smad6, Smad7和Smad8

p-Smad3抗体（1D9）是一种小鼠单克隆IgG抗体，可通过免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测小鼠、大鼠和人类样本中磷酸化Ser 425的Smad3。p-Smad3（1D9）抗体以非结合形式提供，可在研究中广泛使用。Smad3在转化生长因子-β（TGF-β）信号传导通路中起着至关重要的作用，该通路可调节细胞生长、分化及凋亡等多种细胞过程。Smad3在Ser 425位点的磷酸化可增强转录活性，从而影响基因表达对TGF-β信号的反应。这种修饰使Smad3能够与Smad4形成复合物并转移至细胞核，在细胞核中Smad3可调节靶基因的转录。了解Smad3的磷酸化动力学及其在细胞信号传导中的作用，有助于深入了解包括癌症和纤维化在内的各种病理状况，因此抗磷酸化Smad3抗体（1D9）成为研究人员研究这些关键生物学过程的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Smad3 参考文献:

  1. 对活体小鼠中 Smad2/3 依赖性 TGF-beta 信号传导的全面分析表明，组织对损伤有明显的特异性反应。  |  Lin, AH., et al. 2005. J Immunol. 175: 547-54. PMID: 15972691
  2. Smads 和染色质调节  |  van Grunsven, LA., et al. 2005. Cytokine Growth Factor Rev. 16: 495-512. PMID: 15979924
  3. 通过 Smad 途径传递 TGF-beta 信号。  |  Massaous, J. and Hata, A. 1997. Trends Cell Biol. 7: 187-92. PMID: 17708943
  4. 一种作为 BMP 调节的转录激活因子的人类 Mad 蛋白。  |  Liu, F., et al. 1996. Nature. 381: 620-3. PMID: 8637600
  5. MADR2 位于 18q21，编码一种受 TGFbeta 调节的 MAD 相关蛋白，在结直肠癌中发生功能突变。  |  Eppert, K., et al. 1996. Cell. 86: 543-52. PMID: 8752209
  6. 与受体相关的 Mad 同源物协同作用，成为 TGF-beta 反应的效应因子。  |  Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
  7. DPC4 和 SMAD 蛋白在 TGF-beta 信号通路中的合作关系。  |  Lagna, G., et al. 1996. Nature. 383: 832-6. PMID: 8893010
  8. Smad6 可抑制 TGF-beta 超家族的信号传导。  |  Imamura, T., et al. 1997. Nature. 389: 622-6. PMID: 9335505
  9. Smad8介导ALK-2[校正]受体丝氨酸激酶的信号传导。  |  Chen, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12938-43. PMID: 9371779
  10. TGF-beta 信号通过 SMAD 蛋白从细胞膜传递到细胞核。  |  Heldin, CH., et al. 1997. Nature. 390: 465-71. PMID: 9393997

订购信息

产品名称产品编号规格价格数量收藏夹

p-Smad3 抗体 (1D9)

sc-517575
100 µg/ml
RMB2377.00

p-Smad3 (1D9): m-IgG Fc BP-HRP 套装

sc-541114
100 µg Ab; 10 µg BP
RMB2662.00

p-Smad3 (1D9): m-IgG1 BP-HRP 套装

sc-542590
100 µg Ab; 20 µg BP
RMB2662.00

What IgG subtyp is the #sc-517575 antibody?

Asked by: JMM7
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2023-09-20

Does Santa Cruz have any anti-p-Smad antibody suitable for Western Blot Analysis? I think you used to have sc-11769, but it is no longer available. Could you confirm this? Thank you in advance.

Asked by: rrebelo
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2024-05-13

What is best IHC protocol? (with or without HIER, citrate or not, incubation time)

Asked by: QMGC
Thank you for your question. Here is a link to our recommended protocol for IHC: https://www.scbt.com/resources/protocols/immunoperoxidase-staining We do recommend antigen retrieval with sodium citrate. The recommended starting dilution for this antibody is 1:50, and incubation is 1 hour at room temperature. These are starting conditions, optimization will be necessary for each sample.
Answered by: Tech Service
Date published: 2020-02-11
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Rated 5 out of 5 by from clear staining in IFThe antibody was used for Immunofluorescent staining with following confocal microscopy. The signal was clear to identify.
Date published: 2023-08-29
Rated 5 out of 5 by from Great for IHCHi, I have got this antibody to characterise TGFbeta signaling status in liver cancer biopsies. It worked great in IHC using automated Ventana system. Very specific staining with no background.
Date published: 2019-11-13
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p-Smad3 抗体 (1D9) is rated 5.0 out of 5 by 2.
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