Date published: 2026-10-24

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p-Ribosomal Protein S6 Antibody (50.Ser 235/236) - Western Blotting - Image 159480
未经处理 (A, D), 经 EGF 处理 (B, E) 和经 EGF 及 lambda 蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 HEK293 全细胞裂解液中核糖体蛋白 S6 磷酸化的 Western 印迹分析. 测试的抗体包括 p-核糖体蛋白 S6 抗体 (50. Ser 235/236): sc-293144 (A, B, C) 和核糖体蛋白 S6 (C-8): sc-74459 (D, E, F).
未经处理 (A, D), 经 EGF 处理 (B, E) 和经 EGF 及 lambda 蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 HEK293 全细胞裂解液中核糖体蛋白 S6 磷酸化的 Western 印迹分析. 测试的抗体包括 p-核糖体蛋白 S6 抗体 (50. Ser 235/236): sc-293144 (A, B, C) 和核糖体蛋白 S6 (C-8): sc-74459 (D, E, F).

p-Ribosomal Protein S6 抗体 (50.Ser 235/236): sc-293144

4.3(6)
寫評論提問
说明书
  • p-Ribosomal Protein S6抗体(50.Ser 235/236)是小鼠单克隆IgG1 κ, 在31篇文献中引用，规格为200 µg/ml
  • 推荐用于 mouse, rat 和 human 来源的Ser 235 and Ser 236 phosphorylated Ribosomal Protein S6 WB, IP 和 ELISA检测
  • 还可偶联琼脂糖 用于IP; 偶联HRP 用于WB, IHC(P) 和ELISA
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是p-Ribosomal Protein S6 Antibody (50.Ser 235/236) for WB applications. 的首选辅助检测试剂，这些试剂现与p-Ribosomal Protein S6 Antibody (50.Ser 235/236) 打包提供（请参阅下面的订购信息）。
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関連項目

附加Ribosomal Protein抗体，包括Ribosomal Protein L11, Ribosomal Protein L13, Ribosomal Protein L14, Ribosomal Protein L17, Ribosomal Protein L18, Ribosomal Protein L19, Ribosomal Protein L21, Ribosomal Protein L22, Ribosomal Protein L23a, Ribosomal Protein L30, Ribosomal Protein L36a, Ribosomal Protein L39L, Ribosomal Protein L4, Ribosomal Protein L9, Ribosomal Protein LP0和Ribosomal Protein S10

p-核糖体蛋白S6抗体（50.Ser 235/236）是一种IgG1 κ小鼠单克隆p-核糖体蛋白S6抗体，可通过WB、IP和ELISA检测小鼠、大鼠和人源的Ser 235和Ser 236磷酸化的核糖体蛋白S6。p-核糖体蛋白S6抗体（50.Ser 235/236）既可作为偶联抗p-核糖体蛋白S6抗体形式，也可作为非偶联抗p-核糖体蛋白S6抗体形式。编码哺乳动物核糖体蛋白的基因由多基因家族组成，这些基因家族主要由多个加工过的假基因和一个功能性的内含子基因组成。RPS6基因产生核糖体蛋白S6（也称为RPS6）。RPS6是真核核糖体中蛋白激酶的主要底物。序列比较已确定RPS6与酿酒酵母的核糖体蛋白S10相当。来自进化上遥远的真核生物（如酵母和人类）的核糖体蛋白的序列比较表明，RPS6的结构和可能的功能高度保守。RPS6磷酸化受生长因子、肿瘤促进剂和有丝分裂原的刺激。它在生长停滞时被去磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Ribosomal Protein S6 抗体 (50.Ser 235/236) 参考文献:

  1. 来自两种伊蚊的核糖体蛋白 S6 cDNA 编码一个类似组蛋白 H1 蛋白的羧基末端延伸部分。  |  Hernandez, VP. and Fallon, AM. 1999. Genetica. 106: 263-7. PMID: 10897799
  2. 雷帕霉素衍生物RAD001间歇性治疗方案的抗肿瘤功效与外周血单核细胞中核糖体蛋白S6激酶1的长期失活相关。  |  Boulay, A., et al. 2004. Cancer Res. 64: 252-61. PMID: 14729632
  3. 7 个含内含子的核糖体蛋白基因的图谱显示，它们在人类基因组中是不相连的。  |  Feo, S., et al. 1992. Genomics. 13: 201-7. PMID: 1577483
  4. 核糖体蛋白S6激酶与磷酸酶-1激活因子共沉淀。  |  Khatra, BS., et al. 1991. Arch Biochem Biophys. 284: 186-92. PMID: 1989496
  5. TPA诱导的细胞转化引发Pin1和90 kDa核糖体蛋白S6激酶2之间的复合物形成。  |  Cho, YS., et al. 2012. Mol Cell Biochem. 367: 85-92. PMID: 22562304
  6. 鼠李糖核糖体蛋白基因 S6 的一级结构。  |  Gross, T., et al. 1988. Curr Genet. 13: 57-63. PMID: 2834104
  7. 克隆的编码人类核糖体蛋白 S6 的 cDNA 的分离和特征。  |  Lott, JB. and Mackie, GA. 1988. Gene. 65: 31-9. PMID: 2840355
  8. 从克隆的 cDNA 中得到的人类核糖体蛋白 S6 的一级结构。  |  Heinze, H., et al. 1988. J Biol Chem. 263: 4139-44. PMID: 3279029
  9. Ca2+/磷脂依赖性蛋白激酶与其蛋白酶激活形式对核糖体蛋白S6和40-S核糖体亚基的合成肽类似物磷酸化作用的比较研究。  |  Sakanoue, Y., et al. 1987. Eur J Biochem. 168: 669-77. PMID: 3311752
  10. 鉴定瑞士小鼠 3T3 成纤维细胞在血清刺激下的 40 S 核糖体蛋白 S6 磷酸化位点。  |  Bandi, HR., et al. 1993. J Biol Chem. 268: 4530-3. PMID: 8440735

订购信息

产品名称产品编号规格价格数量收藏夹

p-Ribosomal Protein S6 抗体 (50.Ser 235/236)

sc-293144
200 µg/ml
CNY2422.00

p-Ribosomal Protein S6 (50.Ser 235/236): m-IgG Fc BP-HRP 套装

sc-527324
200 µg Ab; 10 µg BP
CNY2715.00

p-Ribosomal Protein S6 (50.Ser 235/236): m-IgG1 BP-HRP 套装

sc-532697
200 µg Ab; 20 µg BP
CNY2715.00

p-Ribosomal Protein S6 抗体 (50.Ser 235/236) AC

sc-293144 AC
500 µg/ml, 25% agarose
CNY3189.00

p-Ribosomal Protein S6 抗体 (50.Ser 235/236) HRP

sc-293144 HRP
200 µg/ml
CNY2422.00

What is the recommended starting dilution for Western blot for sc-293144: p-Ribosomal Protein S6 (50.Ser 235/236) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 2 out of 5 by gkyriakopoulos from No bandsUnfortunately i could not detect any bands in 3% milk/tbst using A549 cell lysates
Date published: 2021-03-17
Rated 5 out of 5 by AleX27 from Excellent antibodyIt worked really well. It does not give any background bands. Perfect!
Date published: 2020-11-11
Rated 5 out of 5 by mcs4 from Excelent resultThe bands were at the expected size and they were nicely visible. Worked great for human fibroblast samples when probing at 1:1000 via WB. Highly recommend!
Date published: 2018-11-08
Rated 5 out of 5 by jerojero from Excellent!This antibody is recommended for detection of Ser 235 and Ser 236 phosphorylated Ribosomal Protein S6 of mouse, rat and human origin by WB, IP and ELISA
Date published: 2017-02-02
Rated 5 out of 5 by CD from WB on Human cancer cell lysates: Very goodWB on Human cancer cell lysates: Very good antibody giving publishable data
Date published: 2016-08-29
Rated 4 out of 5 by LM from Worked well with untreatedWorked well with untreated , EGF treated and EGF and lambda protein phosphatase treated HEK293 whole cell lysates by WB with some background. -SCBT QC
Date published: 2014-01-18
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p-Ribosomal Protein S6 抗体 (50.Ser 235/236) is rated 4.3 out of 5 by 6.
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