Date published: 2025-9-6

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p-Rb 抗体 (E-10): sc-271930

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说明书
  • p-Rb抗体(E-10)是小鼠单克隆IgG3 κ, 在17篇文献中引用,规格为200 µg/ml
  • 推荐用于 mouse, rat 和 human 来源的Thr 821 and Thr 826 dually phosphorylated Rb WB, IP, IF, IHC(P) 和 ELISA检测
  • 抗p-Rb抗体(E-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-Rb (E-10): sc-271930免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是p-Rb Antibody (E-10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与p-Rb Antibody (E-10) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

p-Rb抗体(E-10)是一种IgG3κ小鼠单克隆p-Rb抗体(也称为p-Rb抗体或RB1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Thr 821和Thr 826双磷酸化Rb。p-Rb抗体(E-10)可作为非偶联的抗p-Rb抗体,以及多种偶联形式的抗p-Rb抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。儿童癌症视网膜母细胞瘤和其他人类肿瘤的形成可归因于视网膜母细胞瘤肿瘤抑制基因(Rb)的突变。Rb蛋白通过协调G1-S的细胞周期与转录机制来调节分化、凋亡和细胞周期控制。在G1期,细胞周期蛋白D依赖性激酶介导的Rb在Ser 795的磷酸化标志着Rb从转录抑制性的低磷酸化状态转化为无活性的磷酸化状态,这种状态可能通过多达16个推定的丝氨酸或苏氨酸残基(包括Ser 249/Thr 252、Thr 373、Thr 356、Ser 780、Ser 807/Ser 811和Thr 821/Thr 826)的差异磷酸化维持到有丝分裂。低磷酸化的Rb通过直接的蛋白质-蛋白质相互作用和招募组蛋白脱乙酰酶来抑制控制细胞周期的基因的转录。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

p-Rb 抗体 (E-10)

sc-271930
200 µg/ml
RMB2377.00

p-Rb (E-10): m-IgGκ BP-HRP 套装

sc-522104
200 µg Ab, 40 µg BP
RMB2662.00

p-Rb 抗体 (E-10) AC

sc-271930 AC
500 µg/ml, 25% agarose
RMB3129.00

p-Rb 抗体 (E-10) HRP

sc-271930 HRP
200 µg/ml
RMB2377.00

p-Rb 抗体 (E-10) FITC

sc-271930 FITC
200 µg/ml
RMB2482.00

p-Rb 抗体 (E-10) PE

sc-271930 PE
200 µg/ml
RMB2580.00

p-Rb 抗体 (E-10) Alexa Fluor® 488

sc-271930 AF488
200 µg/ml
RMB2685.00

p-Rb 抗体 (E-10) Alexa Fluor® 546

sc-271930 AF546
200 µg/ml
RMB2685.00

p-Rb 抗体 (E-10) Alexa Fluor® 594

sc-271930 AF594
200 µg/ml
RMB2685.00

p-Rb 抗体 (E-10) Alexa Fluor® 647

sc-271930 AF647
200 µg/ml
RMB2685.00

p-Rb 抗体 (E-10) Alexa Fluor® 680

sc-271930 AF680
200 µg/ml
RMB2685.00

p-Rb 抗体 (E-10) Alexa Fluor® 790

sc-271930 AF790
200 µg/ml
RMB2685.00

p-Rb (E-10) 中和勝肽

sc-271930 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-271930 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good for IHCwe had good results using this ab in IHC, mouse pituitary slices
Date published: 2021-02-11
Rated 4 out of 5 by from works well for WBI used this antibody for WB and obtained good results.
Date published: 2020-08-21
Rated 1 out of 5 by from no bandThere was no band in western-blotting. It does not work.
Date published: 2018-04-18
Rated 4 out of 5 by from Good antibodybought the p-Rb (E-10) antibody about a week ago and it worked quite well with HEK293 cell lysate
Date published: 2018-03-12
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 4 out of 5 by from Nice antibody for western blotThe batch of antibody I have worked well on HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. Observed: a major band between 100-150kDa + one minor band around 37kDa. The antibody was diluted 500 times into 5% BSA, into TBST with an overnight incubation at 4°C.
Date published: 2017-11-16
Rated 4 out of 5 by from GoodSingle band between 100- 130 kDa in 8% PAGE We got this antibody as a sample and used at an dilution of 1:250. Left lane loaded with protein standard marker 250, 130, 100, 55, 35 kDa bands, Right lane loaded 30ug protein lysate Better than many phospho specific antibody.
Date published: 2017-10-27
Rated 5 out of 5 by from Great antibody!1:500 diluted for wb of rat heart protein ,the band is strong and special
Date published: 2017-07-29
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p-Rb 抗体 (E-10) is rated 3.8 out of 5 by 13.
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