Date published: 2025-9-6

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p-Rb 抗体 (B-4): sc-514031

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说明书
  • p-Rb抗体(B-4)是小鼠单克隆IgM κ, 在1篇文献中引用,规格为200 µg/ml
  • 特异性表位,对应于含磷酸化丝氨酸795的短氨基酸序列,Rb human来源
  • 推荐用于 mouse, rat 和 human 来源的Ser 795 phosphorylated Rb WB, IP, IF 和 ELISA检测
  • 可用于ChIP的TransCruz试剂(sc-514031 X, 200 µg/0.1 ml)
  • 关于如何获取p-Rb (B-4): sc-514031免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成p-Rb 抗体 (B-4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

p-Rb抗体(B-4)是一种IgM κ小鼠单克隆p-Rb抗体(也称为p-Rb抗体或RB1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Ser 795磷酸化Rb。p-Rb抗体(B-4)可作为非偶联的抗p-Rb抗体。儿童癌症视网膜母细胞瘤和其他人类肿瘤的形成可归因于视网膜母细胞癌肿瘤抑制基因(Rb)的突变。Rb蛋白通过协调G1-S的细胞周期与转录机制来调节分化、凋亡和细胞周期控制。在G1期,细胞周期蛋白D依赖性激酶介导的Rb在Ser 795的磷酸化标志着Rb从转录抑制性的低磷酸化状态转化为无活性的磷酸化状态,这可以通过多达16个推定的丝氨酸或苏氨酸残基的差异磷酸化通过有丝分裂来维持,包括Ser 249/Thr 252、Thr 373、Thr 356、Ser 780、Ser 807/Ser 811和Thr 821/Thr 826。低磷酸化的Rb通过直接的蛋白质-蛋白质相互作用和通过招募组蛋白脱乙酰酶来抑制控制细胞周期的基因的转录。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

p-Rb 抗体 (B-4)

sc-514031
200 µg/ml
RMB2377.00

p-Rb (B-4) 中和勝肽

sc-514031 P
100 µg/0.5 ml
RMB511.00

p-Rb 抗体 (B-4) X

sc-514031 X
200 µg/0.1 ml
RMB2377.00

What application is the blocking peptide sc-514031 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 2 out of 5 by from Not workIt didn't work for my samples. Not good for MCF7 cells
Date published: 2017-07-30
Rated 5 out of 5 by from GoodThe analysis results of Western blot were very strong and clear,I recommend use it.
Date published: 2017-06-12
Rated 3 out of 5 by from not suitable for turbotthere's no band in turbot. I used 1:100 and 1:2000 for second antibody ,exposed 10mns, no band
Date published: 2017-06-06
Rated 4 out of 5 by from turbotthere are too much band in turbot cell and the signal is very low so i cannot identify it。
Date published: 2017-05-31
Rated 5 out of 5 by from 效果好I have used this product one month ago.It is very clear in western bloting,very good!
Date published: 2017-01-17
Rated 5 out of 5 by from Great Western blot data of Rb phosphorylationGreat Western blot data of Rb phosphorylation expression in MOLT-4 and forskolin treated MOLT-4 whole cell lysates. -SCBT QC
Date published: 2013-02-27
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p-Rb 抗体 (B-4) is rated 3.6 out of 5 by 7.
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