Date published: 2025-9-7
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p-PP2A-Cα/β Antibody (F-8) - Western Blotting - Image 373426
p-PP2A-Cα/β 抗体 (F-8): sc-271903. HeLa (A), IMR-32 (B), Jurkat (C), NIH/3T3 (D), EOC 20 (E) 和 C6 (F) 全细胞裂解液中 PP2A-Cα/β 磷酸化的 Western 印迹分析.
p-PP2A-Cα/β 抗体 (F-8): sc-271903. HeLa (A), IMR-32 (B), Jurkat (C), NIH/3T3 (D), EOC 20 (E) 和 C6 (F) 全细胞裂解液中 PP2A-Cα/β 磷酸化的 Western 印迹分析.

p-PP2A-Cα/β 抗体 (F-8): sc-271903

5.0(8)
寫評論提問

说明书
  • p-PP2A-Cα/β抗体(F-8)是小鼠单克隆IgG1 κ, 在30篇文献中引用，规格为200 µg/ml
  • 针对含磷酸化Tyr 307（PP2A-Cα of mouse起源）的短氨基酸序列
  • p-PP2A-C alpha / beta 抗体 (F-8) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测broad species 来源的 Tyr 307 phosphorylated PP2A-C α and β isoforms
  • 抗p-PP2A-C alpha / beta抗体（F-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-PP2A-Cα/β (F-8): sc-271903的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是p-PP2A-Cα/β Antibody (F-8) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与p-PP2A-Cα/β Antibody (F-8) 搭配使用（请参阅下面的订购信息）。

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说明书与实验方案
研究信息

関連項目

附加PP2抗体，包括PP2A-A α, PP2A-A alpha / β, PP2A-B55, PP2A-B55-α, PP2A-B55-γ, PP2A-B56-α, PP2A-B56-δ, PP2A-B56-γ, PP2A-C, PP2A-C alpha / β, PP2B-A α, PP2B-A β, PP2B-A γ, PP2B-B1, PP2B-B2和PP2C α

p-PP2A-Cα/β 抗体（F-8）是一种 IgG1 κ 小鼠单克隆 p-PP2A-Cα/β 抗体（也称为 p-PP2A 磷酸酶催化亚基 C alpha/beta 抗体），该抗体可通过 WB、IP、IF、IHC(P) 和 ELISA 方法检测 Tyr 307 磷酸化的来自广泛物种的 PP2A-C α 和 β 亚型。p-PP2A-Cα/β 抗体（F-8）既可以是非偶联的抗 p-PP2A-Cα/β 抗体形式，也可以是多种偶联形式的抗 p-PP2A-Cα/β 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。蛋白磷酸酶 2A（PP2A）的催化亚基通过受体和非受体蛋白酪氨酸激酶在体外对 Tyr 307 的磷酸化而失活。PP2A 的催化亚基被酪氨酸特异性蛋白激酶磷酸化，并与多种调节亚基结合。在磷酸酶抑制剂冈田酸存在的情况下，磷酸化作用增强，这与自去磷酸化反应一致。磷酸化由 p60v-src、p56lck、表皮生长因子受体和胰岛素受体催化。PP2A 的瞬时失活可能会增强细胞内激酶级联的细胞信号传导。在真核生物中，蛋白质在丝氨酸和苏氨酸残基上的磷酸化和去磷酸化是调节广泛细胞功能的重要手段，包括细胞分裂、稳态和凋亡。与这一过程密切相关的一组蛋白质是蛋白质磷酸酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-PP2A-Cα/β 抗体 (F-8) 参考文献:

  1. 酪氨酸磷酸化对蛋白丝氨酸-苏氨酸磷酸酶 2A 型的调控。  |  Chen, J., et al. 1992. Science. 257: 1261-4. PMID: 1325671
  2. 小鼠钙调蛋白依赖性蛋白磷酸酶调节亚基（钙调蛋白 B）两种异构体的结构和表达。  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
  3. 磷酸化PP2A（酪氨酸307）与阿尔茨海默氏症神经纤维病理有关。  |  Liu, R., et al. 2008. J Cell Mol Med. 12: 241-57. PMID: 18208556
  4. 在 MC3T3-E1 细胞中，NXNL1 通过 GDF15 诱导的 PP2A Cα 依赖性方式负向调节成骨细胞分化。  |  Kim, KM. and Jang, WG. 2022. Biofactors. 48: 239-248. PMID: 34932831
  5. 线粒体靶向过氧化氢酶通过USP28/miR-200b/PP2A-Cα轴下调p53蛋白稳定性诱导细胞恶性转化  |  Wen, C., et al. 2024. Arch Biochem Biophys. 758: 110047. PMID: 38844154
  6. 蛋白磷酸酶-1 的核抑制剂 NIPP-1 的分子克隆揭示了其与参与 RNA 处理的多肽的同源性。  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
  7. 蛋白磷酸酶 2A 的酪氨酸磷酸化与成纤维细胞的生长刺激和 v-src 转化有关。  |  Chen, J., et al. 1994. J Biol Chem. 269: 7957-62. PMID: 7510677
  8. 丝氨酸/苏氨酸蛋白磷酸酶。  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
  9. 新型蛋白磷酸酶对丝氨酸和苏氨酸残基去磷酸化的重要作用  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
  10. 蛋白丝氨酸/苏氨酸磷酸酶：结构, 调节和在细胞生长中的功能。  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923

订购信息

产品名称产品编号规格价格数量收藏夹

p-PP2A-Cα/β 抗体 (F-8)

sc-271903
200 µg/ml
RMB2377.00

p-PP2A-Cα/β (F-8): m-IgG Fc BP-HRP 套装

sc-525835
200 µg Ab; 10 µg BP
RMB2662.00

p-PP2A-Cα/β 抗体 (F-8) AC

sc-271903 AC
500 µg/ml, 25% agarose
RMB3129.00

p-PP2A-Cα/β 抗体 (F-8) HRP

sc-271903 HRP
200 µg/ml
RMB2377.00

p-PP2A-Cα/β 抗体 (F-8) FITC

sc-271903 FITC
200 µg/ml
RMB2482.00

p-PP2A-Cα/β 抗体 (F-8) PE

sc-271903 PE
200 µg/ml
RMB2580.00

p-PP2A-Cα/β 抗体 (F-8) Alexa Fluor® 488

sc-271903 AF488
200 µg/ml
RMB2685.00

p-PP2A-Cα/β 抗体 (F-8) Alexa Fluor® 546

sc-271903 AF546
200 µg/ml
RMB2685.00

p-PP2A-Cα/β 抗体 (F-8) Alexa Fluor® 594

sc-271903 AF594
200 µg/ml
RMB2685.00

p-PP2A-Cα/β 抗体 (F-8) Alexa Fluor® 647

sc-271903 AF647
200 µg/ml
RMB2685.00

p-PP2A-Cα/β 抗体 (F-8) Alexa Fluor® 680

sc-271903 AF680
200 µg/ml
RMB2685.00

p-PP2A-Cα/β 抗体 (F-8) Alexa Fluor® 790

sc-271903 AF790
200 µg/ml
RMB2685.00

p-PP2A-Cα/β (F-8) 中和勝肽

sc-271903 P
100 µg/0.5 ml
RMB511.00

I have some arabidopsis thaliana samples need to test. Will this antibody work?

Asked by: Anonymous
Thank you for your question. p-PP2A-Cα/β Antibody (F-8) has not yet been tested in arabidopsis thaliana sample and so that is not a recommended species at this time. 感谢您的问题。p-PP2A-Cα/β 抗体 (F-8)还没有检测过拟南芥样本，不推荐检测拟南芥样本。
Answered by: Technical Support 9
Date published: 2020-06-12

Do you have specific technical details about the IHC protocol for this antibody? meaning concentration, kind of antigen retrieval, blocking, etc.

Asked by: AllyValk
Thank you for your question. Please find the link to our IHC protocol below. We recommend starting at a 1:50 primary antibody dilution, sodium citrate with heat at pH 6 for antigen retrieval, and normal serum to block non-specific binding. If you require further assistance, please contact Technical Service. Immunoperoxidase Staining Protocol: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Santa Cruz TS
Date published: 2017-05-19

I have some monkey samples I need to test. Will this antibody work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 4
Date published: 2017-01-13

I would like to use sc-271903 for a double stain. How can I do this when my other antibody is a mouse monoclonal also?

Asked by: Cweed
We recommend using directly conjugated antibodies. This product, sc-271903, is available conjugated to AF488, AF647, and FITC.
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 5 out of 5 by from Worked well!This antibody worked well with our Rat cell line, REF52.
Date published: 2018-08-14
Rated 5 out of 5 by from Clean and strong signal for p-PP2a (F-8)Received a sample of this antibody and found that it strongly detected p-PP2a in rat primary neuronal cultures at a 1:1000 dilution. I recommend this product for WB application.
Date published: 2018-04-06
Rated 5 out of 5 by from perfect antibodyperfect antibody for WB on healthy human liver sample
Date published: 2017-12-04
Rated 5 out of 5 by from pPP2AcI bought pPP2Ac antibody last month and Its work perfectly.
Date published: 2017-10-31
Rated 5 out of 5 by from Detecting P-PP2A-CWe used the antibody (sc-271903) to detect P-PP2A-C in insect samples. The result was quite good.
Date published: 2017-09-11
Rated 5 out of 5 by from Great for IHCThis p-PP2A-C alpha/beta antibody shows good results in IHC of human tissue.
Date published: 2017-01-14
Rated 5 out of 5 by from wonderful Antibody for p-pp2agot the free sample for this P-PP2A one month ago. Tested it. Worked perfect. Placed one full packge order soon.
Date published: 2016-11-11
Rated 5 out of 5 by from Signal via mmunoperoxidase staining of formalinSignal via mmunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of subset of glandular cells. -SCBT QC
Date published: 2013-07-18
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p-PP2A-Cα/β 抗体 (F-8) is rated 5.0 out of 5 by 8.
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