Date published: 2025-9-6

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p-p53 抗体 (FP3.2): sc-51690

4.2(14)
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说明书
  • p-p53抗体(FP3.2)是小鼠单克隆IgG1 κ, 在17篇文献中引用,规格为100 µg/ml
  • 针对与human起源的p53的氨基酸Ser 392对应的C-terminal磷酸化肽
  • 推荐用于 mouse, rat 和 human 来源的Ser 392 phosphorylated p53 WB, IP, IF, IHC(P) 和 ELISA检测; 不建议用于检测dephosphorylated p53
  • 关于如何获取p-p53 (FP3.2): sc-51690免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 p-p53 抗体 (FP3.2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-p53 抗体 (FP3.2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

p-p53抗体(FP3.2)是一种IgG1 κ小鼠单克隆p-p53抗体(也称为TP53抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的Ser 392磷酸化p53。p-p53抗体(FP3.2)可作为非偶联抗p-p53抗体形式存在。p53是一种含有DNA结合、寡聚化域和转录激活域的肿瘤抑制因子,可响应应激信号上调生长停滞和凋亡相关基因,从而影响程序性细胞死亡、细胞分化和细胞周期控制机制。p53定位于细胞核,但可由负调控因子MDM2陪伴至细胞质,MDM2是一种E3泛素连接酶,在活性p53存在的情况下上调,其中MDM2将p53多泛素化以靶向蛋白酶体。在没有DNA的情况下,p53可以组装成四聚体,在潜在的和活性的(DNA结合)构象之间波动,并通过翻译后修饰进行不同的激活,包括磷酸化和乙酰化。p53的DNA结合域(DBD)(氨基酸110-286)突变可以损害与顺式元件的能量有利关联,并与几种人类癌症有关。p53在Thr 155残基的磷酸化由COP9信号体(CSN)介导,并将p53靶向泛素-26S蛋白酶体依赖性降解。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

p-p53 抗体 (FP3.2)

sc-51690
100 µg/ml
RMB2377.00

p-p53 (FP3.2): m-IgG Fc BP-HRP 套装

sc-538943
100 µg Ab; 10 µg BP
RMB2662.00

p-p53 (FP3.2): m-IgG1 BP-HRP 套装

sc-541169
100 µg Ab; 20 µg BP
RMB2662.00

What is the recommended starting dilution for Western blot for p-p53 (FP3.2): sc-51690 monoclonal antibody?

Asked by: Randy McDonald
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-05
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Rated 4 out of 5 by from .Excellent.This antibody works great for western blotting.Excellent.
Date published: 2019-07-29
Rated 5 out of 5 by from Function good for Western Blot (human samples)I used for the detection of p53 after the induction of DNA damage in Molt4 cells (human cell line). I did not detect in the control samples (without treatment) but after the induction of DNA damage I could detect the presence of phosphorylated p53. I used for western blot (dilution 1/1000).
Date published: 2018-02-19
Rated 5 out of 5 by from p-p53 Antibody (FP3.2) very good!strong and clean p-p53 band in human cells treated with DNA damage agents by western blot using a free sample antibody
Date published: 2017-12-08
Rated 4 out of 5 by from bands at the right size on WBseems to be working for western - samples were human cells in culture
Date published: 2017-11-21
Rated 4 out of 5 by from for western blotThis antibody did not work well in mESC samples with 1: 500 dilution for western blot.
Date published: 2017-10-10
Rated 5 out of 5 by from goodI used this sample antibody a month ago and am so happy that I did western blot and got a good result.
Date published: 2017-09-15
Rated 5 out of 5 by from Good antibodyThe antibody Works very well for western blot in our rat lungs tissue
Date published: 2017-08-21
Rated 5 out of 5 by from 好用I get the sample two months ago ,it is very function at WB
Date published: 2017-08-09
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p-p53 抗体 (FP3.2) is rated 4.2 out of 5 by 14.
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