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p-p53 Antibody (F-11) - Western Blotting - Image 296061 — 未经处理 (A, C) 和经λ蛋白磷酸酶 (sc-200312A) 处理 (B, D) 的人重组 p53 中 p53 磷酸化的 Western 印迹分析. 测试的抗体包括 p-p53 抗体 (F-11): sc-377561 (A, B) 和 p53 (Pab 240): sc-99 (C, D).

未经处理 (A, C) 和经λ蛋白磷酸酶 (sc-200312A) 处理 (B, D) 的人重组 p53 中 p53 磷酸化的 Western 印迹分析. 测试的抗体包括 p-p53 抗体 (F-11): sc-377561 (A, B) 和 p53 (Pab 240): sc-99 (C, D).

p-p53 抗体 (F-11): sc-377561

Name: p-p53抗体 (F-11)
Brand: SCBIO
SKU: sc-377561
Price: 2377.0 CNY
Availability: InStock

4.0(4)

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说明书

p-p53抗体(F-11)是小鼠单克隆IgG₁ κ, 在2篇文献中引用，规格为200 µg/ml
特定表位，氨基酸Ser 46 Ser 46之间的映射，p53的human起源
p-p53 抗体 (F-11) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Ser 46 phosporylated p53
抗p-p53抗体（F-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取p-p53 (F-11): sc-377561的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
目前，我们还没有完成p-p53 抗体 (F-11)的首选二抗检测试剂的鉴定。这项工作正在进行中。

查看产品引用文献(2)

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関連項目

p-p53抗体（F-11）是一种IgG1κ小鼠单克隆p-p53抗体（也称为TP53抗体），可通过WB、IP、IF和ELISA检测人源Ser 46磷酸化的p53。p-p53抗体（F-11）既可作为非偶联抗p-p53抗体形式，也可作为多种偶联形式的抗p-p53抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。p53是一种含有DNA结合、寡聚化域和转录激活域的肿瘤抑制因子，可上调生长停滞和凋亡相关基因以响应应激信号，从而影响程序性细胞死亡、细胞分化和细胞周期控制机制。p53定位于细胞核，但可由负调节因子MDM2陪伴至细胞质。MDM2是一种E3泛素连接酶，在活性p53存在时上调，其中它使p53多泛素化以靶向蛋白酶体。p53在潜伏和活性DNA结合构象之间波动，并通过包括磷酸化和乙酰化在内的翻译后修饰进行差异激活。p53的DNA结合域（DBD）中的突变（氨基酸110-286）会损害与顺式元件的能量有利关联，并涉及几种人类癌症。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

LI-COR^®和 Odyssey^® 是LI-COR Biosciences的注册商标

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
p-p53 抗体 (F-11)	sc-377561	200 µg/ml	RMB2377.00
p-p53 抗体 (F-11) AC	sc-377561 AC	500 µg/ml, 25% agarose	RMB3129.00
p-p53 抗体 (F-11) HRP	sc-377561 HRP	200 µg/ml	RMB2377.00
p-p53 抗体 (F-11) FITC	sc-377561 FITC	200 µg/ml	RMB2482.00
p-p53 抗体 (F-11) PE	sc-377561 PE	200 µg/ml	RMB2580.00
p-p53 抗体 (F-11) Alexa Fluor^® 488	sc-377561 AF488	200 µg/ml	RMB2685.00
p-p53 抗体 (F-11) Alexa Fluor^® 546	sc-377561 AF546	200 µg/ml	RMB2685.00
p-p53 抗体 (F-11) Alexa Fluor^® 594	sc-377561 AF594	200 µg/ml	RMB2685.00
p-p53 抗体 (F-11) Alexa Fluor^® 647	sc-377561 AF647	200 µg/ml	RMB2685.00
p-p53 抗体 (F-11) Alexa Fluor^® 680	sc-377561 AF680	200 µg/ml	RMB2685.00
p-p53 抗体 (F-11) Alexa Fluor^® 790	sc-377561 AF790	200 µg/ml	RMB2685.00
p-p53 (F-11) 中和勝肽	sc-377561 P	100 µg/0.5 ml	RMB511.00

This Ab is also available conjugated to Alexa Fluor® 488 and does it work for flow cytometry? What is the protocol recommended to stain intracellular phosphorylated p53? Thank you.

Asked by: Karine2023

Thank you for your question. AlexaFluor conjugates of this antibody can be used in flow cytometry. You can find our recommended protocol under this link: https://www.scbt.com/resources/protocols/flow-cytometry

Answered by: Tech Support Europe

Date published: 2023-02-16

I wonder why the band size of this antibody (p-p53 Antibody (F-11): sc-377561) is at 89KDa when the predicted molercular weight is 53KDa. I bought this antibody and I get a band at around 100KDa?

Asked by: HRC1

Thank you for your question. The expected molecular weight of p-p53, as detected by p-p53 Antibody (F-11): sc-377561, is 53 kDa. If you are detecting a signal at a different height, please contact our technical service for assistance.

Answered by: SCBT Heidelberg Support

Date published: 2023-05-24

What application is the blocking peptide sc-377561 P appropriate for?

Asked by: TinTin

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Support

Date published: 2017-02-28

Rated 4 out of 5 by SPJB from Worked well for WB analysisTried 1:1000 dilution in human bladder cancer cell lines - worked well for WB analysis, strong signal

Date published: 2018-04-03

Rated 4 out of 5 by Abreporter from a relatively good antibody for WBThis antibody works for WB (1:500 dilution, 40 ug HeLa Lysate).

Date published: 2017-07-05

Rated 3 out of 5 by crl1215 from Nice productIt showed slight background but I still got my results. Thanks for this sample product.

Date published: 2017-05-04

Rated 5 out of 5 by HF from Good WB dataGood WB data, phosphorylated p53 clearly detected in recombinant p53.

Date published: 2013-12-10

p-p53 抗体 (F-11) is rated 4.0 out of 5 by 4.

Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。

p-p53 抗体 (F-11): sc-377561

p-p53 抗体 (F-11) 规格

替代产品名称: TP53

产品克隆名称: F-11

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: Ser 46

抗原表位靶标: p53

抗原表位种属: human

产品反应性/检测: Ser 46 phosporylated p53

种属反应性/检测: human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 2篇各种科学出版物的引文

優選的 p53 抗體是 p53 抗體 (DO-1): sc-126.

p-p53 抗体 (F-11)

p-p53 抗体 (F-11) AC

p-p53 抗体 (F-11) HRP

p-p53 抗体 (F-11) FITC

p-p53 抗体 (F-11) PE

p-p53 抗体 (F-11) Alexa Fluor^® 488

p-p53 抗体 (F-11) Alexa Fluor^® 546

p-p53 抗体 (F-11) Alexa Fluor^® 594

p-p53 抗体 (F-11) Alexa Fluor^® 647

p-p53 抗体 (F-11) Alexa Fluor^® 680

p-p53 抗体 (F-11) Alexa Fluor^® 790

p-p53 (F-11) 中和勝肽

This Ab is also available conjugated to Alexa Fluor® 488 and does it work for flow cytometry? What is the protocol recommended to stain intracellular phosphorylated p53? Thank you.

I wonder why the band size of this antibody (p-p53 Antibody (F-11): sc-377561) is at 89KDa when the predicted molercular weight is 53KDa. I bought this antibody and I get a band at around 100KDa?

What application is the blocking peptide sc-377561 P appropriate for?

p-p53 抗体 (F-11): sc-377561

p-p53 抗体 (F-11) 规格

替代产品名称: TP53

产品克隆名称: F-11

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸: Ser 46

抗原表位靶标: p53

抗原表位种属: human

产品反应性/检测: Ser 46 phosporylated p53

种属反应性/检测: human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 2篇各种科学出版物的引文

優選的 p53 抗體是 p53 抗體 (DO-1): sc-126.

p-p53 抗体 (F-11)

p-p53 抗体 (F-11) AC

p-p53 抗体 (F-11) HRP

p-p53 抗体 (F-11) FITC

p-p53 抗体 (F-11) PE

p-p53 抗体 (F-11) Alexa Fluor® 488

p-p53 抗体 (F-11) Alexa Fluor® 546

p-p53 抗体 (F-11) Alexa Fluor® 594

p-p53 抗体 (F-11) Alexa Fluor® 647

p-p53 抗体 (F-11) Alexa Fluor® 680

p-p53 抗体 (F-11) Alexa Fluor® 790

p-p53 (F-11) 中和勝肽

This Ab is also available conjugated to Alexa Fluor&reg; 488 and does it work for flow cytometry? What is the protocol recommended to stain intracellular phosphorylated p53? Thank you.

I wonder why the band size of this antibody (p-p53 Antibody (F-11): sc-377561) is at 89KDa when the predicted molercular weight is 53KDa. I bought this antibody and I get a band at around 100KDa?

What application is the blocking peptide sc-377561 P appropriate for?

抗体同型: IgG₁ kappa light chain

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

p-p53 抗体 (F-11) Alexa Fluor^® 488

p-p53 抗体 (F-11) Alexa Fluor^® 546

p-p53 抗体 (F-11) Alexa Fluor^® 594

p-p53 抗体 (F-11) Alexa Fluor^® 647

p-p53 抗体 (F-11) Alexa Fluor^® 680

p-p53 抗体 (F-11) Alexa Fluor^® 790

This Ab is also available conjugated to Alexa Fluor® 488 and does it work for flow cytometry? What is the protocol recommended to stain intracellular phosphorylated p53? Thank you.