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p-p53 Antibody (B-3) - Western Blotting - Image 289902 — 未经处理 (A, D), ERK2 处理 (B, E) 以及 ERK2 和 lambda 蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的人重组 p53 中 p53 磷酸化的 Western 印迹分析. 测试的抗体包括 p-p53 抗体 (B-3): sc-377553. (A, B, C) 和 p53 (Pab 240): sc-99 (D, E, F).

未经处理 (A, D), ERK2 处理 (B, E) 以及 ERK2 和 lambda 蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的人重组 p53 中 p53 磷酸化的 Western 印迹分析. 测试的抗体包括 p-p53 抗体 (B-3): sc-377553. (A, B, C) 和 p53 (Pab 240): sc-99 (D, E, F).

p-p53 抗体 (B-3): sc-377553

Name: p-p53抗体 (B-3)
Brand: SCBIO
SKU: sc-377553
Price: 2377.0 CNY
Availability: InStock

3.6(5)

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说明书

p-p53抗体(B-3)是小鼠单克隆IgG₁ κ, 在2篇文献中引用，规格为200 µg/ml
与human起源的短氨基酸序列（含Thr 55、磷酸化p53）杂交
p-p53 抗体 (B-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Thr 55 phosphorylated p53
抗p-p53抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取p-p53 (B-3): sc-377553的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP 和 m-IgG₁ BP-HRP 是 p-p53 抗体 (B-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-p53 抗体 (B-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

p-p53抗体（B-3）是一种IgG1κ小鼠单克隆p-p53抗体（也称为TP53抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人源Thr 55磷酸化的p53。p-p53抗体（B-3）既可作为非偶联抗p-p53抗体形式，也可作为多种偶联形式的抗p-p53抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。p53是一种含有DNA结合、寡聚化域和转录激活域的肿瘤抑制因子，可上调生长停滞和凋亡相关基因以响应应激信号，从而影响程序性细胞死亡、细胞分化和细胞周期控制机制。p53定位于细胞核，但可由负调节因子MDM2陪伴至细胞质，MDM2是一种E3泛素连接酶，在活性p53存在时上调，其中MDM2使p53多泛素化以靶向蛋白酶体。在没有DNA的情况下，p53可以组装成四聚体，在潜伏和活性（DNA结合）构象之间波动，并通过包括磷酸化和乙酰化在内的翻译后修饰进行差异激活。p53的DNA结合域（DBD）（氨基酸110-286）中的突变会损害与顺式元件的能量有利关联，并涉及几种人类癌症。p53在残基Thr 155处的磷酸化由COP9信号小体（CSN）介导，并将p53靶向泛素-26S蛋白酶体依赖性降解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

LI-COR^®和 Odyssey^® 是LI-COR Biosciences的注册商标

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
p-p53 抗体 (B-3)	sc-377553	200 µg/ml	RMB2377.00
p-p53 (B-3): m-IgG Fc BP-HRP 套装	sc-527610	200 µg Ab; 10 µg BP	RMB2662.00
p-p53 (B-3): m-IgG₁ BP-HRP 套装	sc-532983	200 µg Ab; 20 µg BP	RMB2662.00
p-p53 抗体 (B-3) AC	sc-377553 AC	500 µg/ml, 25% agarose	RMB3129.00
p-p53 抗体 (B-3) HRP	sc-377553 HRP	200 µg/ml	RMB2377.00
p-p53 抗体 (B-3) FITC	sc-377553 FITC	200 µg/ml	RMB2482.00
p-p53 抗体 (B-3) PE	sc-377553 PE	200 µg/ml	RMB2580.00
p-p53 抗体 (B-3) Alexa Fluor^® 488	sc-377553 AF488	200 µg/ml	RMB2685.00
p-p53 抗体 (B-3) Alexa Fluor^® 546	sc-377553 AF546	200 µg/ml	RMB2685.00
p-p53 抗体 (B-3) Alexa Fluor^® 594	sc-377553 AF594	200 µg/ml	RMB2685.00
p-p53 抗体 (B-3) Alexa Fluor^® 647	sc-377553 AF647	200 µg/ml	RMB2685.00
p-p53 抗体 (B-3) Alexa Fluor^® 680	sc-377553 AF680	200 µg/ml	RMB2685.00
p-p53 抗体 (B-3) Alexa Fluor^® 790	sc-377553 AF790	200 µg/ml	RMB2685.00
p-p53 (B-3) 中和勝肽	sc-377553 P	100 µg/0.5 ml	RMB511.00

Hello, We are conducting a western blot using fibroblast cells and we are using the p-p53 antibodies (B-3): sc-377553 to detect phosphorylated p53 (Thr 55). We were wondering what size the bands show up as since we don't really detect 50 kDa bands.

Asked by: Anonymous

Thank you for your question. The expected molecular weight of p-p53 is 53 kDa. If issues in your experiment persist, please reach out to our technical service team for direct troubleshooting assistance.

Answered by: Tech Support Europe

Date published: 2023-01-16

This antibody (sc-377553) seems to recognize a higher band than predicted (around 90 kDa vs 55 kDa). I was wondering if you could comment on that. Other antibodies against P-p53 seem to detect a protein around 55 kDa.

Asked by: gmercenne

Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.

Answered by: Blake J

Date published: 2021-11-09

What application is the blocking peptide sc-377553 P appropriate for?

Asked by: TinTin

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Support

Date published: 2017-03-01

Rated 3 out of 5 by jylang from 抗体的非特异信号多我们使用这个抗体做western 实验，实验结果显示背景特别高，主带信号弱，杂带很多。尽管我们使用了BSA 作为封闭剂，且封闭2个小时，但是杂带还是很多。

Date published: 2017-09-21

Rated 3 out of 5 by Abreporter from an OK antibody for WBThis antibody works for WB (1:500 dilution, 40 ug HeLa Lysate), although the signal intensity is low (and also with a higher background). However, we used frozen cell lysates and have not tested fresh samples.

Date published: 2017-07-05

Rated 3 out of 5 by crl1215 from The performance is okThe antibody can be better. I got a few non-specific bands and slight background but still got my results.

Date published: 2017-05-04

Rated 4 out of 5 by dolphin11065 from goodwork well in WB at 1:250, Gastric cancer cell lines

Date published: 2017-01-10

Rated 5 out of 5 by JM from Works great for Western blot in recombinant p53Works great for Western blot in recombinant p53. -SCBT QC

Date published: 2014-08-10

p-p53 抗体 (B-3) is rated 3.6 out of 5 by 5.

Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。

p-p53 抗体 (B-3): sc-377553

p-p53 抗体 (B-3) 规格

替代产品名称: TP53

产品克隆名称: B-3

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: Thr 55

抗原表位靶标: p53

抗原表位种属: human

产品反应性/检测: Thr 55 phosphorylated p53

种属反应性/检测: human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 2篇各种科学出版物的引文

優選的 p53 抗體是 p53 抗體 (DO-1): sc-126.

p-p53 抗体 (B-3)

p-p53 (B-3): m-IgG Fc BP-HRP 套装

p-p53 (B-3): m-IgG₁ BP-HRP 套装

p-p53 抗体 (B-3) AC

p-p53 抗体 (B-3) HRP

p-p53 抗体 (B-3) FITC

p-p53 抗体 (B-3) PE

p-p53 抗体 (B-3) Alexa Fluor^® 488

p-p53 抗体 (B-3) Alexa Fluor^® 546

p-p53 抗体 (B-3) Alexa Fluor^® 594

p-p53 抗体 (B-3) Alexa Fluor^® 647

p-p53 抗体 (B-3) Alexa Fluor^® 680

p-p53 抗体 (B-3) Alexa Fluor^® 790

p-p53 (B-3) 中和勝肽

Hello, We are conducting a western blot using fibroblast cells and we are using the p-p53 antibodies (B-3): sc-377553 to detect phosphorylated p53 (Thr 55). We were wondering what size the bands show up as since we don't really detect 50 kDa bands.

This antibody (sc-377553) seems to recognize a higher band than predicted (around 90 kDa vs 55 kDa). I was wondering if you could comment on that. Other antibodies against P-p53 seem to detect a protein around 55 kDa.

What application is the blocking peptide sc-377553 P appropriate for?

p-p53 抗体 (B-3): sc-377553

p-p53 抗体 (B-3) 规格

替代产品名称: TP53

产品克隆名称: B-3

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸: Thr 55

抗原表位靶标: p53

抗原表位种属: human

产品反应性/检测: Thr 55 phosphorylated p53

种属反应性/检测: human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 2篇各种科学出版物的引文

優選的 p53 抗體是 p53 抗體 (DO-1): sc-126.

p-p53 抗体 (B-3)

p-p53 (B-3): m-IgG Fc BP-HRP 套装

p-p53 (B-3): m-IgG1 BP-HRP 套装

p-p53 抗体 (B-3) AC

p-p53 抗体 (B-3) HRP

p-p53 抗体 (B-3) FITC

p-p53 抗体 (B-3) PE

p-p53 抗体 (B-3) Alexa Fluor® 488

p-p53 抗体 (B-3) Alexa Fluor® 546

p-p53 抗体 (B-3) Alexa Fluor® 594

p-p53 抗体 (B-3) Alexa Fluor® 647

p-p53 抗体 (B-3) Alexa Fluor® 680

p-p53 抗体 (B-3) Alexa Fluor® 790

p-p53 (B-3) 中和勝肽

Hello, We are conducting a western blot using fibroblast cells and we are using the&nbsp;p-p53 antibodies (B-3): sc-377553 to detect phosphorylated p53 (Thr 55). We were wondering what size the bands show up as since we don't really detect 50 kDa bands.

This antibody (sc-377553) seems to recognize a higher band than predicted (around 90 kDa vs 55 kDa). I was wondering if you could comment on that. Other antibodies against P-p53 seem to detect a protein around 55 kDa.

What application is the blocking peptide sc-377553 P appropriate for?

抗体同型: IgG₁ kappa light chain

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

p-p53 (B-3): m-IgG₁ BP-HRP 套装

p-p53 抗体 (B-3) Alexa Fluor^® 488

p-p53 抗体 (B-3) Alexa Fluor^® 546

p-p53 抗体 (B-3) Alexa Fluor^® 594

p-p53 抗体 (B-3) Alexa Fluor^® 647

p-p53 抗体 (B-3) Alexa Fluor^® 680

p-p53 抗体 (B-3) Alexa Fluor^® 790

Hello, We are conducting a western blot using fibroblast cells and we are using the p-p53 antibodies (B-3): sc-377553 to detect phosphorylated p53 (Thr 55). We were wondering what size the bands show up as since we don't really detect 50 kDa bands.