Date published: 2025-9-6

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p-p53 抗体 (59.Ser 315): sc-135772

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说明书
  • p-p53抗体(59.Ser 315)是小鼠单克隆IgG2a κ, 在7篇文献中引用,规格为200 µg/ml
  • 针对human起源的p53的Ser 315磷酸化的短氨基酸序列
  • 推荐用于 human 来源的Ser 315 phosphorylated p53 WB, IP 和 ELISA检测
  • 还可偶联琼脂糖 用于IP; 偶联HRP 用于WB, IHC(P) 和ELISA
  • 可用于ChIP的TransCruz试剂(sc-135772 X, 200 µg/0.1 ml)
  • 关于如何获取p-p53 (59.Ser 315): sc-135772免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是p-p53 Antibody (59.Ser 315) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与p-p53 Antibody (59.Ser 315) 打包提供(请参阅下面的订购信息)。

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関連項目

p-p53抗体(59.Ser 315)是一种IgG2aκ小鼠单克隆p-p53抗体(也称为TP53抗体),可通过WB、IP和ELISA检测人源Ser 315磷酸化的p53。p-p53抗体(59.Ser 315)可作为偶联和非偶联抗p-p53抗体形式使用。p53是一种含有DNA结合、寡聚化域和转录激活域的肿瘤抑制因子,可根据应激信号上调生长停滞和凋亡相关基因,从而影响程序性细胞死亡、细胞分化和细胞周期控制机制。p53定位于细胞核,但可被负调控因子MDM2(一种在活性p53存在时上调的E3泛素连接酶)伴侣至细胞质,其中MDM2使p53多泛素化以靶向蛋白酶体。在没有DNA的情况下,p53可以组装成四聚体,在潜伏和活性(DNA结合)构象之间波动,并通过包括磷酸化和乙酰化在内的翻译后修饰进行差异激活。p53的DNA结合域(DBD)(氨基酸110-286)中的突变可以损害与顺式元件的能量有利关联,并与几种人类癌症有关。p53在残基Thr 155处的磷酸化由COP9信号体(CSN)介导,并将p53靶向泛素-26S蛋白酶体依赖性降解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

p-p53 抗体 (59.Ser 315)

sc-135772
200 µg/ml
$316.00

p-p53 (59.Ser 315): m-IgG Fc BP-HRP 套装

sc-528791
200 µg Ab; 10 µg BP
$354.00

p-p53 (59.Ser 315): m-IgGκ BP-HRP 套装

sc-521300
200 µg Ab, 40 µg BP
$354.00

p-p53 (59.Ser 315): m-IgG2a BP-HRP 套装

sc-547171
200 µg Ab; 10 µg BP
$354.00

p-p53 抗体 (59.Ser 315) X

sc-135772 X
200 µg/0.1 ml
$316.00

p-p53 抗体 (59.Ser 315) AC

sc-135772 AC
500 µg/ml, 25% agarose
$416.00

p-p53 抗体 (59.Ser 315) HRP

sc-135772 HRP
200 µg/ml
$316.00

When should the TransCruz reagent sc-135772 X be used instead of the standard form sc-135772?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great band with little background!We used this antibody in SW480 whole cell lysate, incubated in 5% BSA at 1:500 dilution. It worked great!
Date published: 2018-03-17
Rated 5 out of 5 by from Worked perfectlyWe used this antibody to test the stress activity in cardiomyocytes and to my surprise we got a single p53 band. Perfect antibody
Date published: 2018-02-01
Rated 5 out of 5 by from Great antibodyUsed it on mitochondrial extract at 1:250 and that was quiet strong for this antibody. The Molecular size is fine and so is activity.
Date published: 2017-08-16
Rated 4 out of 5 by from a good antibody for WBWe got a reasonably good and clean signal for WB using Odyssey buffer and TBS wash (1:500 dilution, 40 ug HeLa lysate, overnight incubation in the cold room)
Date published: 2017-07-11
Rated 4 out of 5 by from GreatThis Ab is working very fine for mouse spleen WB. used 1:500
Date published: 2017-05-26
Rated 3 out of 5 by from Band is strong in human transfected lysatesBand is strong in human transfected lysates. -SCBT QC
Date published: 2014-02-02
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p-p53 抗体 (59.Ser 315) is rated 4.3 out of 5 by 6.
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