Date published: 2025-9-6

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p-RELA/NFκB p65 抗体 (A-8): sc-166748

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说明书
  • p-RELA/NFκB p65抗体(A-8)是小鼠单克隆IgM κ, 在80篇文献中引用,规格为200 µg/ml
  • 推荐用于 mouse, rat 和 human 来源的Ser 311 phosphorylated NFκB p65 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取p-RELA/NFκB p65 (A-8): sc-166748免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成p-RELA/NFκB p65 抗体 (A-8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

p-RELA/NFκB p65 抗体 (A-8) 是一种 IgM κ 小鼠单克隆 p-NFκB p65 抗体(也称为 p-NFκB p65、RELA、RelA、p65、NFKB3 抗体),它通过蛋白质印迹法(WB)、免疫沉淀法(IP)、免疫荧光法(IF)、免疫组化(IHC-P)和酶联免疫吸附测定法(ELISA)检测小鼠、大鼠和人类来源的 Ser 311 磷酸化的 NFκB p65。p-RELA/NFκB p65 抗体 (A-8) 可作为非偶联的抗 p-NFκB p65 抗体形式提供。由 v-Rel 病毒癌基因及其细胞同源物 c-Rel 编码的蛋白质是转录因子家族的成员,该家族包括转录因子 NFκB(p50 和 p65)的两个亚基和果蝇母体形态发生因子 dorsal。这两种蛋白质都特异性地结合到免疫球蛋白 κ 轻链增强子中的 10 bp κB 序列或该序列的轻微变体的相同或相似的 DNA 序列上。该序列也存在于许多其他细胞和病毒增强子中。在暴露于有丝分裂原或生长因子的细胞中,NFκB 的 DNA 结合活性被激活,随后 NFκB 从细胞质转运到细胞核。已经描述了编码两种不同蛋白质前体的 cDNA,分别命名为 p105 和 p100。p105 前体在其 N 端包含 p50,并在 C 端区域(当作为单独分子表达时,命名为 pdI)与 p50 结合并调节其活性。NFκB 转录因子是由 DNA 结合亚基和相关蛋白组成的蛋白质复合物。DNA 结合亚基,也称为 Rel A,与 c-Rel p75 和 RelB p68 功能相关。NFκB p65 在丝氨酸-311 处作为对蛋白激酶 C ζ 的响应而被磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

p-RELA/NFκB p65 抗体 (A-8)

sc-166748
200 µg/ml
RMB2377.00

p-RELA/NFκB p65 (A-8) 中和勝肽

sc-166748 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-166748 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2020-10-07
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Rated 5 out of 5 by from western blotThe antibody can gave a nice clean band. It is a good antibody.
Date published: 2023-03-10
Rated 2 out of 5 by from not good for wbThe antibody was used in triple negative breast cancer cell lines and in colon cancer cell lines at the concentration of 1:500 diluted in TBS 0,1% Tween-20 and used overnight. The signal was low, not specific and other bands are visible
Date published: 2021-10-04
Rated 4 out of 5 by from Excellent.This antibody works great for western blotting.Excellent.
Date published: 2019-07-30
Rated 4 out of 5 by from okthe antibody works in mice liver lysates, dilution 1:500
Date published: 2018-09-05
Rated 4 out of 5 by from Works fine in WBAntibody works good in western blot and acceptable in IF
Date published: 2018-05-30
Rated 5 out of 5 by from Works well in WB.Works well with poly I:C stimulated fibroblast lysate in 1:200 dilution.
Date published: 2018-05-17
Rated 4 out of 5 by from great antibodypNFKB antibody on porcine lung samples at 1:1000, it worked well. thanks
Date published: 2018-05-11
Rated 5 out of 5 by from it works well on western blotit has a good expression on human skins tissue for western blot
Date published: 2017-10-27
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p-RELA/NFκB p65抗体 (A-8) is rated 4.1 out of 5 by 22.
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