Date published: 2025-9-6
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p-MEK-3/6 Antibody (B-9) - Western Blotting - Image 355249
p-MEK-3/6 抗体 (B-9): sc-8407. COLO 320DM (A), K-562 (B), NIH/3T3 (C), RAW 264. 7 (D), KNRK (E) 和 L8 (F) 全细胞裂解液中 MEK-3/6 磷酸化的 Western 印迹分析.
p-MEK-3/6 抗体 (B-9): sc-8407. COLO 320DM (A), K-562 (B), NIH/3T3 (C), RAW 264. 7 (D), KNRK (E) 和 L8 (F) 全细胞裂解液中 MEK-3/6 磷酸化的 Western 印迹分析.

p-MEK-3/6 抗体 (B-9): sc-8407

4.8(13)
寫評論提問

说明书
  • p-MEK-3/6抗体(B-9)是小鼠单克隆IgG1 κ, 在22篇文献中引用，规格为200 µg/ml
  • 抗-p-MEK-3/6 抗体 (B-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MEK-3 and MEK-6 phosphorylated at Ser 189 and Ser 207
  • 抗p-MEK-3/6抗体（B-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-MEK-3/6 (B-9): sc-8407的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 p-MEK-3/6 抗体 (B-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-MEK-3/6 抗体 (B-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 MEK 抗体 包括 MEK-1, MEK-2, MEK-3, MEK-4, MEK-5, MEK-6和MEK-7

p-MEK-3/6抗体（B-9）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过应用诸如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶 酶联免疫吸附测定（ELISA）。p-MEK-3/6 (B-9) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物，可满足不同实验需求。MEK-3，也称为MKK-3，在激活p38 MAP激酶通路中起着至关重要的作用，该通路对于细胞对压力和炎症的反应至关重要。MEK-3主要位于细胞质中，在信号转导通路中充当关键介质，调节细胞生长、分化与存活。MEK-3的正常运作至关重要，因为失调会导致包括癌症和炎症在内的多种疾病。p-MEK-3/6 (B-9) 单克隆抗体使研究人员能够深入了解MEK-3的激活状态及其在这些关键细胞过程中的参与情况。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-MEK-3/6 抗体 (B-9) 参考文献:

  1. MEK 的主要结构，MEK 是一种蛋白激酶，能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  2. 慢性咀嚼肌痛中涉及 MEK3-p38 MAPK 激活的分子变化  |  Meng, H., et al. 2016. J Dent Res. 95: 1169-75. PMID: 27418173
  3. 一种新的人类蛋白激酶信号转导途径的组成部分。  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
  4. 由 MEK 和 MKK 同工酶定义的独立人类 MAP 激酶信号转导途径。  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
  5. 一种新的哺乳动物丝裂原活化蛋白激酶激酶 MKK2 的鉴定和表征。  |  Wu, J., et al. 1993. Mol Cell Biol. 13: 4539-48. PMID: 8393135
  6. 新型 MAP 激酶激酶 (MKK6) 的结构和功能特性。  |  Han, J., et al. 1996. J Biol Chem. 271: 2886-91. PMID: 8621675
  7. 一种新的丝裂原活化蛋白激酶（p38beta）的结构和功能特征。  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
  8. 丝裂原活化蛋白激酶激酶 7 是 c-Jun NH2 端激酶的激活剂。  |  Tournier, C., et al. 1997. Proc Natl Acad Sci U S A. 94: 7337-42. PMID: 9207092
  9. MKK7 是一种应激激活的丝裂原活化蛋白激酶激酶，在功能上与半蝶呤有关。  |  Holland, PM., et al. 1997. J Biol Chem. 272: 24994-8. PMID: 9312105
  10. 人 JNKK2（一种新型 Jun NH2-末端激酶特异性激酶）的分子克隆和特征描述。  |  Wu, Z., et al. 1997. Mol Cell Biol. 17: 7407-16. PMID: 9372971

订购信息

产品名称产品编号规格价格数量收藏夹

p-MEK-3/6 抗体 (B-9)

sc-8407
200 µg/ml
RMB2377.00

p-MEK-3/6 (B-9): m-IgG Fc BP-HRP 套装

sc-528224
200 µg Ab; 10 µg BP
RMB2662.00

p-MEK-3/6 (B-9): m-IgGκ BP-HRP 套装

sc-520550
200 µg Ab, 40 µg BP
RMB2662.00

p-MEK-3/6 (B-9): m-IgG1 BP-HRP 套装

sc-542825
200 µg Ab; 20 µg BP
RMB2662.00

p-MEK-3/6 抗体 (B-9) AC

sc-8407 AC
500 µg/ml, 25% agarose
RMB3129.00

p-MEK-3/6 抗体 (B-9) HRP

sc-8407 HRP
200 µg/ml
RMB2377.00

p-MEK-3/6 抗体 (B-9) FITC

sc-8407 FITC
200 µg/ml
RMB2482.00

p-MEK-3/6 抗体 (B-9) PE

sc-8407 PE
200 µg/ml
RMB2580.00

p-MEK-3/6 抗体 (B-9) Alexa Fluor® 488

sc-8407 AF488
200 µg/ml
RMB2685.00

p-MEK-3/6 抗体 (B-9) Alexa Fluor® 546

sc-8407 AF546
200 µg/ml
RMB2685.00

p-MEK-3/6 抗体 (B-9) Alexa Fluor® 594

sc-8407 AF594
200 µg/ml
RMB2685.00

p-MEK-3/6 抗体 (B-9) Alexa Fluor® 647

sc-8407 AF647
200 µg/ml
RMB2685.00

p-MEK-3/6 抗体 (B-9) Alexa Fluor® 680

sc-8407 AF680
200 µg/ml
RMB2685.00

p-MEK-3/6 抗体 (B-9) Alexa Fluor® 790

sc-8407 AF790
200 µg/ml
RMB2685.00

p-MEK-3/6 (B-9) 中和勝肽

sc-8407 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-8407 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Great for WBIt is a good antibody in WB. It works in dilution 1:1000.
Date published: 2024-03-20
Rated 5 out of 5 by from ExcellentI bought this antibody a few years ago and the result is good and clear.
Date published: 2021-11-08
Rated 5 out of 5 by from p-MEK3/6 antibodyThis antibody is good for western blotting and present data is clear
Date published: 2018-09-28
Rated 5 out of 5 by from Great antibody for WBI also test this antibody with liver cells and the results are so good
Date published: 2017-10-29
Rated 5 out of 5 by from Highly potent and specific antibodyThis monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is highly recommended for Western blotting. There was very strong detection of MKK3 in SDS-PAGE sample buffer solubilized lysates from human HeLa cells treated for 2 hours with anisomycin on Western blots, but no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2016-12-04
Rated 5 out of 5 by from Publishable Western blot results using humanPublishable Western blot results using human breast cancer cells. -SCBT Publication Review
Date published: 2015-05-03
Rated 5 out of 5 by from WB Published with this antibody using humanWB Published with this antibody using human fibroblast cell extracts for immunoblot analysis -SCBT Publication Review
Date published: 2015-03-24
Rated 5 out of 5 by from Great antibody for Western BlotGreat antibody for Western Blot, positive signal obtained in human MEK-3 transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-02-26
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p-MEK-3/6 抗体 (B-9) is rated 4.8 out of 5 by 13.
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