Date published: 2025-9-6

021-6093-6350

SCBT Portrait Logo
Seach Input

p-MEK-1 抗体 (47.Ser 222): sc-136542

3.2(5)
寫評論提問

说明书
  • p-MEK-1 抗体 47.Ser 222 是小鼠单克隆 IgA, 在4篇文献中引用,提供 200 µg/ml
  • 与包含human起源的MEK-1的磷酸化丝氨酸222的短氨基酸序列
  • 推荐用于 Ser 222 phosphorylated MEK-1 and Ser 222 phosphorylated MEK-2 of mouse, rat and human origin WB, IP 和 ELISA检测
  • 关于如何获取p-MEK-1 (47.Ser 222): sc-136542免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成p-MEK-1 抗体 (47.Ser 222)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

関連項目

p-MEK-1抗体(47.Ser 222)是一种IgA小鼠单克隆p-MEK-1抗体(也称为p-MEK-1抗体或MAP2K1抗体),它通过蛋白质印迹法(WB)、免疫沉淀法(IP)和酶联免疫吸附测定法(ELISA)检测小鼠、大鼠和人类来源的Ser 222磷酸化MEK-1和Ser 222磷酸化MEK-2。p-MEK-1抗体(47.Ser 222)以非偶联形式的抗p-MEK-1抗体存在。已经鉴定出位于MAP激酶上游并负责其激活的一组蛋白激酶。该家族的原型成员被命名为MAP激酶激酶或MEK-1,它特异性地磷酸化MAP激酶调节性苏氨酸和酪氨酸残基,这些残基存在于ERK的Thr-Glu-Tyr模体中。第二个MEK家族成员MEK-2在底物特异性上与MEK-1相似。MEK-3(或MKK-3)的功能是激活p38 MAP激酶,而MEK-4(也称为SEK1或MKK-4)则激活p38和JNK MAP激酶。MEK-5似乎特异性地磷酸化ERK5,而MEK-6则磷酸化p38和p38β。MEK-7(或MKK-7)磷酸化并激活JNK信号转导途径。MAP激酶激酶激酶(RAF或MEKK1)对Ser/Thr的磷酸化正向调节激酶活性。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

p-MEK-1 抗体 (47.Ser 222)

sc-136542
200 µg/ml
RMB2377.00

What is the recommended starting dilution for Western blot for p-MEK-1 (47.Ser 222): sc-136542 monoclonal antibody?

Asked by: jerojero
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-136542.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_136542, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 95ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Not goodI tested it in our Hela cell lysate, and it did not work at all
Date published: 2018-04-03
Rated 4 out of 5 by from Working good!I got this for the free sample trial, and treated on B16 cells. It worked well.
Date published: 2018-03-07
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from Publishable Western blot results using humanPublishable Western blot results using human osteosarcoma cell lines (U2OS and HOS). -SCBT Publication Review
Date published: 2015-01-23
Rated 5 out of 5 by from Good WB publishable data gathered with humanGood WB publishable data gathered with human primary osteoblasts and Synovial fibroblasts. -SCBT Publication Review
Date published: 2014-05-24
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_zh_CN, sid_136542, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT
p-MEK-1 抗体 (47.Ser 222) is rated 3.2 out of 5 by 5.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_zh_CN, sid_136542, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 111ms
  • REVIEWS, PRODUCT