Date published: 2025-9-6
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p-JNK Antibody (G-7) - Immunohistochemistry - Image 348288
p-JNK 抗体 (G-7): sc-6254. 福尔马林固定, 石蜡包埋的人乳腺组织的免疫过氧化物酶染色, 显示腺细胞和肌上皮细胞的胞浆和膜染色.
p-JNK 抗体 (G-7): sc-6254. 福尔马林固定, 石蜡包埋的人乳腺组织的免疫过氧化物酶染色, 显示腺细胞和肌上皮细胞的胞浆和膜染色.

p-JNK 抗体 (G-7): sc-6254

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说明书
  • p-JNK抗体(G-7)是小鼠单克隆IgG1 κ, 在1,454篇文献中引用，规格为200 µg/ml
  • 抗-p-JNK 抗体 (G-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Thr 183 and Tyr 185 phosphorylated JNK1, JNK2 and JNK3
  • 抗p-JNK抗体（G-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取p-JNK (G-7): sc-6254的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 p-JNK 抗体 (G-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-JNK 抗体 (G-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 JNK 抗体包括 JNK1, JNK2 和 JNK3

p-JNK抗体（G-7）是一种小鼠单克隆IgG1 kappa轻链抗体，针对磷酸化人c-Jun N-末端激酶（p-JNK）产生，后者是一种应激激活蛋白激酶，对细胞应激反应至关重要。p-JNK小鼠单克隆抗体（G-7）可识别 磷酸化后的JNK，并表现出广泛的物种交叉反应性，可通过Western blotting (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、免疫组织化学 (IHCP) 和酶联免疫吸附测定 (ELISA) 应用检测小鼠、大鼠和人类样本中的磷酸化JNK。抗-p-JNK单克隆抗体（G-7）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种Alexa Fluor®结合物，可满足各种实验需求。JNK家族包括三个成员--JNK1、JNK2和JNK3，它们通过Thr183和Tyr185处的复杂双磷酸化机制激活。这些特定的磷酸化反应由JNK激活激酶在细胞分裂素活化蛋白激酶（MAPK）级联中催化，使JNK能够有效应对各种细胞应激，尤其是紫外线照射。一旦激活，p-JNK就会在Ser63和Ser73处对c-Jun进行磷酸化，从而增强c-Jun调节基因表达的能力。这种磷酸化级联反应对于控制细胞过程至关重要，包括应激和炎症信号引起的细胞增殖、分化及凋亡。通过这些不同的调节作用，p-JNK信号显著影响正常细胞功能和包括癌症和神经退行性疾病在内的各种病理状况的发展。p-JNK (G-7)抗体是研究这些复杂的信号通路及其在疾病机制中的影响的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-JNK 抗体 (G-7) 参考文献:

  1. JNKK2-JNK1 融合蛋白是一种组成型活性 c-Jun 激酶，可刺激 c-Jun 的转录活性。  |  Zheng, C., et al. 1999. J Biol Chem. 274: 28966-71. PMID: 10506143
  2. 肿瘤蛋白介导的信号级联通过丝氨酸 63 和 73 的磷酸化刺激 c-Jun 的活性。  |  Smeal, T., et al. 1992. Mol Cell Biol. 12: 3507-13. PMID: 1630458
  3. Pro-Leu-Ser/Thr-Pro 是底物蛋白磷酸化的共识主序列。表皮生长因子受体苏氨酸 669 蛋白激酶对 c-myc 和 c-jun 蛋白磷酸化的表征。  |  Alvarez, E., et al. 1991. J Biol Chem. 266: 15277-85. PMID: 1651323
  4. MAP 激酶介导的 c-jun 磷酸化。  |  Pulverer, BJ., et al. 1991. Nature. 353: 670-4. PMID: 1922387
  5. ERK：丝氨酸/苏氨酸蛋白激酶家族，在胰岛素和 NGF 的作用下被激活并酪氨酸磷酸化。  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
  6. 评估口腔上皮细胞发育不良中p-AKT、p-JNK和FoxO3a的功能。  |  Chaves, FN., et al. 2017. Oral Dis. 23: 367-378. PMID: 27976455
  7. 抑制 p-mTOR 可通过降低 p-JNK 抑制 PS1 的转录  |  Das, HK. and Hontiveros, SS. 2020. Front Biosci (Landmark Ed). 25: 1297-1304. PMID: 32114433
  8. 丹酚酸 B通过下调TLR4、p-p38MAPK、p-JNK、NF-κB和IL-1β对小鼠脑缺血再灌注损伤的神经保护机制  |  Zheng, XF., et al. 2023. Immun Inflamm Dis. 11: e1030. PMID: 37904689
  9. JNK1：一种受紫外线和 Ha-Ras 刺激的蛋白激酶，能与 c-Jun 激活结构域结合并使其磷酸化。  |  Dérijard, B., et al. 1994. Cell. 76: 1025-37. PMID: 8137421
  10. c-Jun 激酶的应激活化蛋白激酶亚家族。  |  Kyriakis, JM., et al. 1994. Nature. 369: 156-60. PMID: 8177321

订购信息

产品名称产品编号规格价格数量收藏夹

p-JNK 抗体 (G-7)

sc-6254
200 µg/ml
RMB2377.00

p-JNK (G-7): m-IgG Fc BP-HRP 套装

sc-528163
200 µg Ab; 10 µg BP
RMB2662.00

p-JNK (G-7): m-IgGκ BP-HRP 套装

sc-520471
200 µg Ab, 40 µg BP
RMB2662.00

p-JNK (G-7): m-IgG1 BP-HRP 套装

sc-542793
200 µg Ab; 20 µg BP
RMB2662.00

p-JNK 抗体 (G-7) AC

sc-6254 AC
500 µg/ml, 25% agarose
RMB3129.00

p-JNK 抗体 (G-7) HRP

sc-6254 HRP
200 µg/ml
RMB2377.00

p-JNK 抗体 (G-7) FITC

sc-6254 FITC
200 µg/ml
RMB2482.00

p-JNK 抗体 (G-7) PE

sc-6254 PE
200 µg/ml
RMB2580.00

p-JNK 抗体 (G-7) Alexa Fluor® 488

sc-6254 AF488
200 µg/ml
RMB2685.00

p-JNK 抗体 (G-7) Alexa Fluor® 546

sc-6254 AF546
200 µg/ml
RMB2685.00

p-JNK 抗体 (G-7) Alexa Fluor® 594

sc-6254 AF594
200 µg/ml
RMB2685.00

p-JNK 抗体 (G-7) Alexa Fluor® 647

sc-6254 AF647
200 µg/ml
RMB2685.00

p-JNK 抗体 (G-7) Alexa Fluor® 680

sc-6254 AF680
200 µg/ml
RMB2685.00

p-JNK 抗体 (G-7) Alexa Fluor® 790

sc-6254 AF790
200 µg/ml
RMB2685.00

p-JNK 抗体 (G-7) B

sc-6254 B
200 µg/ml
RMB2407.00

p-JNK 抗体 (G-7) TRITC

sc-6254 TRITC
200 µg/ml
RMB2407.00

p-JNK (G-7) 中和勝肽

sc-6254 P
100 µg/0.5 ml
RMB511.00

Can this product detect phosphorylated JNK species in rat brain via histology? Could you also provide a couple of citations where it has been used for this purpose

Asked by: Fred27
Thank you for your question. p-JNK (G-7) reacts with Tyr 185 phosphorylated JNK1, JNK2 and JNK3. PMID : 34206129 PMID : 3174849 PMID : 30469316
Answered by: BlakeJ
Date published: 2023-06-14

Hello, can i use this Antibody to detect phosphorylation in primary human fibroblasts?

Asked by: Alexander M
Thank you for your question. p-JNK Antibody (G-7): sc-6254 detects Thr 183 and Tyr 185 phosphorylated JNK1, JNK2 and JNK3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. It is only specific against these phosphorylated residues and does not detect JNK that does not possess these phosphorylations.
Answered by: Tech Support Europe
Date published: 2019-08-29

Hi. Hope you are well. I am planning to use p-JNK antibody in western blotting in mice brain areas of hippocampus, cortex and nucleus accumbens. Is this product suitable for it as I cannot tell from the references. Thanks

Asked by: Lalji
Thank you for your question. Yes, this antibody is recommended for Western blotting in samples of mouse origin and is specific for phosphorylated JNK. There are product citations specifically in mouse brain. Please contact our Technical Service Department if you would like us to provide specific product citations as a reference.
Answered by: Technical Support
Date published: 2019-05-07

can I use sc-6254 PE in mouse cells for flow cytometry. ....any publication data?

Asked by: Thom
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-01-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-14

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Cweed
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. SC-6254 is currently available conjugated to AF488, AF647, and FITC.
Answered by: TechService7
Date published: 2016-12-14
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Rated 5 out of 5 by from Western blotThe antibody can give a nice clean band. It is a good antibody.
Date published: 2024-07-01
Rated 5 out of 5 by from Great for WBIt is a good antibody for western blot. I'd recommend it.
Date published: 2024-03-19
Rated 5 out of 5 by from Anti-JNK antibody works well for WBAnti-JNK antibody works well for WB in MCF10A cells.
Date published: 2024-01-11
Rated 4 out of 5 by from Good antibody-p-JNKRelatively good antibody-p-JNK for me on western blot.
Date published: 2021-12-03
Rated 5 out of 5 by from ExcellentI bought this antibody a few years ago and the result is good and clear.
Date published: 2021-11-08
Rated 5 out of 5 by from great antibody!This primary antibody is useful in western blotting
Date published: 2021-02-08
Rated 5 out of 5 by from Sc-6254 - p-JNK Antibody ReviewThe p-JNK Antibody (Sc-6254) was used for Western Blot analysis and Immunohistochemistry analysis and gives excellent data in the same.
Date published: 2020-10-20
Rated 4 out of 5 by from Good for WBThis antibody is high specific and high sensitivity in human liver cell lines. Highly recommend antibody
Date published: 2019-11-02
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p-JNK抗体 (G-7) is rated 4.7 out of 5 by 73.
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