Date published: 2025-9-6
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p-NFKBIA/IkB alpha Antibody (B-9) - Western Blotting - Image 1057
未经处理 (A, C) 和 TNF-α 诱导 (B, D) 的 HeLa 细胞中 IκB-α 磷酸化的 Western 印迹分析. 测试的抗体包括 p-IκB-α 抗体 (B-9): sc-8404 (A, B) 和 IκB-α (H-4): sc-1643 (C, D).
p-NFKBIA/IkB alpha Antibody (B-9) - Western Blotting - Image 1057
未经处理 (A, C) 和 TNF-α 诱导 (B, D) 的 HeLa 细胞中 IκB-α 磷酸化的 Western 印迹分析. 测试的抗体包括 p-IκB-α 抗体 (B-9): sc-8404 (A, B) 和 IκB-α (H-4): sc-1643 (C, D).
p-NFKBIA/IkB alpha Antibody (B-9) - Immunofluorescence - Image 160128
p-IkB-a. (B-9): sc-8404. 甲醇固定, TNFa 处理的 HeLa 细胞的免疫荧光染色, 显示活化的 IkB-a 的核定位.
p-NFKBIA/IkB alpha Antibody (B-9) - Immunohistochemistry - Image 160127
p-IkB-a 抗体 (B-9): sc-8404. 福尔马林固定, 石蜡包埋的人乳腺癌的免疫过氧化物酶染色, 显示活化 IkB-a 的核定位.
未经处理 (A, C) 和 TNF-α 诱导 (B, D) 的 HeLa 细胞中 IκB-α 磷酸化的 Western 印迹分析. 测试的抗体包括 p-IκB-α 抗体 (B-9): sc-8404 (A, B) 和 IκB-α (H-4): sc-1643 (C, D).

p-NFKBIA/IkB alpha 抗体 (B-9): sc-8404

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寫評論提問

说明书
  • p-NFKBIA/IkB alpha抗体(B-9)是小鼠单克隆IgG2b κ, 在523篇文献中引用，规格为200 µg/ml
  • 抗-p-NFKBIA/IkB alpha 抗体 (B-9) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 IκB-α phosphorylated at Ser 32
  • 抗p-NFKBIA/IkB alpha抗体（B-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-NFKBIA/IkB alpha (B-9): sc-8404的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 p-NFKBIA/IkB alpha 抗体 (B-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-NFKBIA/IkB alpha 抗体 (B-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 I κ B 抗体 包括 I kappa B-α, I kappa B-β和I κ B-ε

p-IκB-α抗体（B-9）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过应用诸如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织 化学。p-IκB-α (B-9) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。IκB-α在调节NF-κB信号通路中起着至关重要的作用，该通路控制免疫反应、炎症和细胞存活。通过将NF-κB二聚体隔离在细胞质中，IκB-α阻止其转移到细胞核，从而抑制参与这些过程的靶基因的转录。IκB-α在Ser 32位点的磷酸化标志着IκB-α的降解，从而允许NF-κB进入细胞核并激活基因表达。了解IκB-α的磷酸化动态对于研究各种疾病至关重要，包括癌症和自身免疫性疾病，在这些疾病中，NF-κB信号通常失调。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-NFKBIA/IkB alpha 抗体 (B-9) 参考文献:

  1. I kappa B gamma，一种与 p110 NF-kappa B C-terminal half 相同的 70 kd 蛋白：I kappa B 家族的新成员。  |  Inoue, J., et al. 1992. Cell. 68: 1109-20. PMID: 1339305
  2. 粘附单核细胞中诱导的编码 I kappa B 样活性的即时早期基因的特征。  |  Haskill, S., et al. 1991. Cell. 65: 1281-9. PMID: 1829648
  3. Rel-associated pp40：rel 家族转录因子的抑制剂。  |  Davis, N., et al. 1991. Science. 253: 1268-71. PMID: 1891714
  4. 与 Rel 相关的 pp40 蛋白可阻止 Rel 和 NF-kappa B 的 DNA 结合：与 I kappa B beta 的关系以及磷酸化的调节。  |  Kerr, LD., et al. 1991. Genes Dev. 5: 1464-76. PMID: 1907941
  5. NF-kappa B 抑制剂 I kappa B 的磷酸化在体外激活 NF-kappa B。  |  Ghosh, S. and Baltimore, D. 1990. Nature. 344: 678-82. PMID: 2157987
  6. 磷酸化酪氨酸与蛋白质的结合：磷酸化IκB-α的体外翻译及其与NF-κB的相互作用研究。  |  Chen, S., et al. 2017. J Am Chem Soc. 139: 14098-14108. PMID: 28898075
  7. Nur77 通过抑制帕金森病细胞模型中磷酸化的 IκB-α 减轻炎症反应和氧化应激。  |  Yan, J., et al. 2020. Aging (Albany NY). 12: 8107-8119. PMID: 32401747
  8. I kappa B-beta调节NF-kappa B双相激活中的持续反应。  |  Thompson, JE., et al. 1995. Cell. 80: 573-82. PMID: 7867065
  9. I kappa B家族的新成员I kappa B epsilon控制着RelA和cRel NF-kappa B的活性。  |  Whiteside, ST., et al. 1997. EMBO J. 16: 1413-26. PMID: 9135156
  10. 小鼠 IkappaBepsilon 的克隆和功能鉴定。  |  Simeonidis, S., et al. 1997. Proc Natl Acad Sci U S A. 94: 14372-7. PMID: 9405619

订购信息

产品名称产品编号规格价格数量收藏夹

p-NFKBIA/IkB alpha 抗体 (B-9)

sc-8404
200 µg/ml
RMB2377.00

p-NFKBIA/IkB alpha (B-9): m-IgG Fc BP-HRP 套装

sc-528222
200 µg Ab; 10 µg BP
RMB2662.00

p-NFKBIA/IkB alpha (B-9): m-IgGκ BP-HRP 套装

sc-520548
200 µg Ab, 40 µg BP
RMB2662.00

p-NFKBIA/IkB alpha 抗体 (B-9) AC

sc-8404 AC
500 µg/ml, 25% agarose
RMB3129.00

p-NFKBIA/IkB alpha 抗体 (B-9) HRP

sc-8404 HRP
200 µg/ml
RMB2377.00

p-NFKBIA/IkB alpha 抗体 (B-9) FITC

sc-8404 FITC
200 µg/ml
RMB2482.00

p-NFKBIA/IkB alpha 抗体 (B-9) PE

sc-8404 PE
200 µg/ml
RMB2580.00

p-NFKBIA/IkB alpha 抗体 (B-9) Alexa Fluor® 488

sc-8404 AF488
200 µg/ml
RMB2685.00

p-NFKBIA/IkB alpha 抗体 (B-9) Alexa Fluor® 546

sc-8404 AF546
200 µg/ml
RMB2685.00

p-NFKBIA/IkB alpha 抗体 (B-9) Alexa Fluor® 594

sc-8404 AF594
200 µg/ml
RMB2685.00

p-NFKBIA/IkB alpha 抗体 (B-9) Alexa Fluor® 647

sc-8404 AF647
200 µg/ml
RMB2685.00

p-NFKBIA/IkB alpha 抗体 (B-9) Alexa Fluor® 680

sc-8404 AF680
200 µg/ml
RMB2685.00

p-NFKBIA/IkB alpha 抗体 (B-9) Alexa Fluor® 790

sc-8404 AF790
200 µg/ml
RMB2685.00

p-NFKBIA/IkB alpha (B-9) 中和勝肽

sc-8404 P
100 µg/0.5 ml
RMB511.00

How does addition of HRP to primary Ab affect the WB protocol? Other words what is difference between SC-8404 and SC-8404 HRP? Thank you!

Asked by: Sasha K
Thank you for your question. The difference between sc-8404 and sc-8404 HRP is that the latter is the primary antibody directly conjugated to horseradish peroxidase (HRP). If using a primary antibody conjugated to HRP, then it is not necessary to use an HRP conjugated secondary antibody for detection in Western blot. Instead, you can incubate the membrane with HRP conjugated primary antibody, and then proceed directly to incubating the membrane in Chemiluminescence Luminol Reagent (sc-2048) and visualization using your standard protocols. Please contact our Technical Service Department if you would like to further discuss the advantages and disadvantages of using either the conjugated, or unconjugated primary antibody for your application. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected], or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-05-05

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anna99
We recommend trying one of our new Mouse IgG Binding Proteins. This is a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 5
Date published: 2016-12-16

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anna99
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody for p-IκB-α is currently available conjugated to agarose (sc-8404 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-8404 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-8404 PE), fluorescein (sc-8404 FITC), Alexa Fluor® 488 (sc-8404 AF488) or Alexa Fluor® 647 (sc-8404 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 5
Date published: 2016-12-16
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Rated 5 out of 5 by from GoodGreat antibody for WB, works in mouse RAW 264.7 cells
Date published: 2025-03-18
Rated 4 out of 5 by from Works fineDetects a stimulus-specific band at a MW slightly higher than the expected. The cell line of choice may not be the most sensitive, however.
Date published: 2023-05-16
Rated 1 out of 5 by from Showing much larger band size than predictedI bought this antibody 3 weeks ago, I cannot detect the western blot band at the predicted size (40-45 kDa) even when I used positive control, but I got the much larger protein band around 80-90 kDa instead. I used 3% BSA in TBST for blocking and washed for 10 minutes 3 times. It seems like some people from the old comments also have this problem. Can anybody help?
Date published: 2022-07-02
Rated 5 out of 5 by from Very good Ab!The sc-8404 antibody was tested (1: 200, ON 4°) on samples extracted from rat hippocampus using a 12% acrylamide gel. The antibody worked very well. In figure (from left to right): 30ug, 60ug, 80ug and 100ug [marker (from the bottom): 46,57 kDa]
Date published: 2022-06-23
Rated 2 out of 5 by from No signalNo signal was detected but no antibody was left to try other conditions. The conditions used were 1:100 3% v/w milk/TBST and overnight incubation
Date published: 2021-03-17
Rated 4 out of 5 by from Buena señal.Bandas detectadas en peso molecular. Muestras de células estrelladas.
Date published: 2020-05-09
Rated 2 out of 5 by from week signal and additional bandI have used this antibody on mouse brain lysate and I get a very week although detectable signal. However I am struggeling with an additional band around 65-70 kDa. Infact this higher band shows greater response to ischemia than the specific band at the right size, which is quite surprising.
Date published: 2019-09-20
Rated 5 out of 5 by from WB result is goodI bought the product one month ago, and the WB result is clear and repeatable.
Date published: 2019-04-23
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p-NFKBIA/IkB alpha 抗体 (B-9) is rated 4.3 out of 5 by 50.
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