Date published: 2025-9-8
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p-Histone H3 Antibody (HTA28) - Western Blotting - Image 349171
组蛋白 H3 抗体 (HTA28): sc-56745. 对 Jurkat (A) 和 Calyculin A 处理过的 Jurkat (B) 全细胞裂解液中组蛋白 H3 磷酸化的 Western 印迹分析.
组蛋白 H3 抗体 (HTA28): sc-56745. 对 Jurkat (A) 和 Calyculin A 处理过的 Jurkat (B) 全细胞裂解液中组蛋白 H3 磷酸化的 Western 印迹分析.

p-Histone H3 抗体 (HTA28): sc-56745

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说明书
  • p-Histone H3 抗体 HTA28 是大鼠单克隆 IgG2a, 在2篇文献中引用，提供 200 µg/ml
  • 免疫human物种的p-Histone H3的氨基酸Ser 28
  • 推荐用于 mouse, rat, human 和 bovine 来源的Ser 28 phosphorylated Histone H3 WB, IP, IF 和 FCM检测; 不建议用于检测the unphosphorylated epitope
  • 看 p-Histone H3 (C-2): sc-374669，可查看有标记的 p-Histone H3 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成p-Histone H3 抗体 (HTA28)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

附加Histone抗体，包括Histone cluster 1 H1, Histone H2A, Histone H2B, Histone H3, Histone H4和Histone cluster 1 H3A

在真核生物中，DNA缠绕在组蛋白八聚体上，形成染色质结构的基本单位。八聚体由组蛋白H2A、H2B、H3和H4组成，它与大约200个碱基对的DNA结合形成核小体。DNA与组蛋白的结合导致染色质密集包装，这限制了参与基因转录的蛋白质与DNA的结合。核小体的核心蛋白组蛋白H3在前期结束时被磷酸化。磷酸化的两个主要位点是丝裂原特异性位点Ser 10和Ser 28，两者在DNA结合形式的组蛋白H3和核小体组蛋白H3中都广泛磷酸化。组蛋白H3的核小体结构促进了体外N末端磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

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p-Histone H3 抗体 (HTA28)

sc-56745
200 µg/ml
RMB2377.00

Are there any issues with using sc-56745: p-Histone H3 (HTA28) mouse monoclonal primary antibody on mouse samples?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-56745 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Expected results by Western blot analysisExpected results by Western blot analysis of Histone H3 phosphorylation in HeLa and Calyculin A-treated HeLa whole cell lysates. - SCBT QC
Date published: 2015-04-06
Rated 3 out of 5 by from Satisfactory Western blot analysis of HistoneSatisfactory Western blot analysis of Histone H3 phosphorylation in untreated, calyculin A treated and calyculin A and lambda protein phosphatase treated HeLa whole cell lystates. Some background - SCBT QC
Date published: 2015-01-24
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p-Histone H3 抗体 (HTA28) is rated 4.0 out of 5 by 2.
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